혈액 지문 증강을 위한 Natural Yellow 3의 Acid Yellow 7에 대한 대체가능성에 관한 연구
A Preliminary Study on a New Natural Yellow 3 Reagent for Enhancing Faint Bloody Fingermarks
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초록
범죄현장에서 발견되는 혈액지문은 범죄수사에 있어 강력한 의미를 지닌다. 하지만 혈액지문이 어둡거나 복잡한 배경을 지닌 표면 등에 희미하게 남아있다면 증강을 통해 식별 가능한 상태로 만들어야 수사에 활용할 수 있다. 이 경우, acid yellow 7(AY7)을 사용하여 혈액을 형광으로 증강하면 식별이 가능해진다. 그러나 AY7은 혈액의 양이 많을수록 형광의 세기는 어두워지며 적용시간이 타 기법에 비해 길다는 단점이 있다. 본 연구에서는 비다공성 표면에 남겨진 혈액지문을 형광으로 증강하기 위해 natural yellow 3(NY3)가 AY7을 대체할 수 있는지 알아보았다. NY3의 최적의 조성을 탐색하기 위해 증류수와 에탄올의 비율, 산의 종류 및 적정 pH를 실험하였으며, 그 결과물을 AY7과 비교하였다. 그 결과 에탄올과 증류수의 비율이 5:5이며 acetic acid로 pH를 2로 맞춘 NY3 조성이 혈액지문을 효과적으로 현출해냈으며, AY7보다 형광이 밝고 적용시간도 10초 내외로 더 짧았다.
Abstract
Fingermark in blood found at crime scenes have strong implications for criminal investigations. However, if the bloody fingermark remain faintly on surfaces with dark or multi-colored backgrounds, they must be enhanced to be used for investigation. In this case, the blood may be enhanced to fluorescence using acid yellow 7 (AY7). However, AY7 has the disadvantage that the higher the blood volume, the lower the fluorescence intensity, and the longer the application time compared to other techniques. In this study, we investigated whether natural yellow 3 (NY3) can replace AY7 as a method for fluorescence enhancement of faint bloody fingermark left on non-porous surfaces. For the optimal composition of NY3, the ratio of distilled water to ethanol, the type of acid, and the appropriate pH were searched, and the results were compared with AY7. As a result, the ratio of ethanol to distilled water was 5:5, and the NY3 composition with a pH of 2 with acetic acid effectively enhanced bloody fingermarks, and the fluorescence was brighter than AY7 and the application time was very short, about 10 seconds.
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