본문 바로가기
KERD
논문KCI 등재

사료 내 단백질 수준 및 Protease 첨가가 이유자돈의 사양성적, 영양소 소화율, 장 내 미생물, 소장 형태학적 변화 및 분 중 유해가스 발생에 미치는 영향

Effects of Dietary Protein Level and Supplementation of Protease on Growth Performance, Nutrient Digestibility, Gut Microflora, Intestinal Morphology, and Fecal Noxious Gas Emission in Weanling Pigs

김진수|최요한|문준영|박종완|사수진|하상훈|Abdolreza Hossseindoust|하동진|Joseph Moturi|Tajudeen Habeeb|이창번
동물생명과학연구소 (Institute of Animal Life Science)|2021.09|동물자원연구 (ANNALS OF ANIMAL RESOURCE SCIENCES)|제32권 제3호|pp. 48-56|DOI 10.12718/AARS.2021.32.3.48

2

조회

0

다운로드

1

인용

0

참고문헌

키워드

ProteinProteaseNoxious gasNutrient digestibilityweanling pigs
주제분류축산학

초록

본 연구는 사료 내 단백질 수준 및 protease 첨가가 이유자돈의 사양성적, 영양소 소화율, 장 내 미생물, 소장 형태학적 변화 및 분 중 유해가스 발생에 미치는 영향을 알아보기 위해 실시되었다. 총 240두의 이유자돈(Landrace×Yorkshire×Duroc, 5.82±0.3kg)을 공시였으며, 4주 간 2단계(days 0-14, phase 1; and days 15-28, phase 2)로 나누어 사양실험을 진행하였다. 처리구는 사료 내 단백질 두 수준과(phase 1, HP: CP 21% / LP: CP 19%; phase 2, HP: CP 19% / LP: CP 17%) protease 첨가 유무에 따른 2×2 요인설계법에 준하였다. 연구 결과, 일당증체량은 LP 처리구(357g/d)가 HP처리구(339g/d)에 비하여 증가하였고(p<0.05), 사료 내 protease 첨가한 처리구(358g/d)가 비첨가구(339g/d)에 비하여 증가하였다(p<0.05). 일당사료섭취량은 LP 처리구(554g/d)가 HP(530g/d) 처리구에 비하여 증가하였고(p<0.05), 사료 내 protease 첨가한 처리구(552g/d)가 비첨가구(523g/d)에 비하여 증가하였다(p<0.05). 사료 내 protease 첨가구의 건물소화율과 조단백질 소화율(각각 82.62%, 76.08%)이 비첨가구(각각 81.74%, 75.13%)에 비해 증가되었고(p<0.05), 사료 내 protease 첨가구의 Ileum Lactobacillus spp. 수치(7.42log10CFU/g)가 비첨가구(7.32log10CFU/g)에 비하여 증가되었다(p<0.05). 또한 LP 처리구의 암모니아 발생량(4.41ppm)이 HP처리구(4.78ppm)에 비하여 감소하였고(p<0.05), 사료 내 protease 첨가한 처리구의 황화수소 발생량(10.43ppm)이 비첨가구(11.76ppm)에 비하여 감소하였다(p<0.05). 본 연구결과로 미루어 볼 때, 이유자돈 사료 내 단백질 함량 감소 및 protease 첨가 시 자돈의 성장, 영양소 소화율 및 분 중 유해가스 발생량 저감에 도움이 되는 것으로 판단되며 이로 인해 사료비 절감 및 환경 개선의 효과를 얻을 수 있을 것으로 사료된다.

Abstract

This study was conducted to determine the effects of dietary protein level and supplementation of protease on growth performance, nutrient digestibility, gut microflora, intestinal morphology and fecal noxious gas emission in weanling pigs. A total of 240 weaned pigs (Landrace×Yorkshire×Duroc, 5.82±0.3 kg) were used during 4 weeks in 2 phases (days 0-14, phase 1; and days 15-28, phase 2) feeding program based on age and initial body weight. Pigs were allocated to 2×2 factorial arrangement, including 2 protein levels (HP, high protein; LP, low protein) and 2 protease levels (with or without protease). The average daily gain in the LP treatment (357g/d) was increased rather than the HP treatment (339g/d). A greater avarage daily gain was observed in dietary suppiemented protease treatment (358 vs 339g/d). Average feed intake was greater in the LP treatment (544g/d) rather than the HP treatment (530g/d). A greater average daily feed intake was observed in dietary supplemented protease treatment (552 vs 523g/d). Dry matter and crude protein digestibility were increased in dietary supplemented protease treatment (82.62% and 76.08%, respectively) rather than non-supplemented treatment (81.74% and 75.13%, respectively). Ileal Lactobacillus spp. count increased in dietary supplemented protease treatment (7.42 vs 7.32log10CFU/g). Emission of H2S was decreased in the LP treatment (4.41ppm) rather than HP treatment (4.78ppm). Emission of NH3 was decreased in dietary supplemented protease treatment (10.43ppm vs 11.76ppm). In conclusion, the decrease of dietary protein level and supplementation of protease had beneficial effects on growth performance, nutrient digestibility, gut microflora, and noxious gas emission in weanling pigs.

참고문헌 (27)

  1. AOAC, "Official Methods of Analysis" (2007), Association of Official Analytical Chemists International
  2. Bhandari, S. K., "Dietary protein level and probiotic supplementation effects on piglet response to Escherichia coli K88 challenge: performance and gut microbial population", Livest. Sci, 133(1-3), pp.185~188 (2010)
  3. Chen, F., "Soybean-derived β-conglycinin affects proteome expression in pig intestinal cells in vivo and in vitro", J. Anim. Sci, 89(3), pp.743~753 (2011)
  4. Duarte, M. E., "Dietary supplementation of xylanase and protease on growth performance, digesta viscosity, nutrient digestibility, immune and oxidative stress status, and gut health of newly weaned pigs", Anim. Nutr, 5(4), pp.351~358 (2019)
  5. 홍성민, "복합효소제의 첨가가 육성돈의 생산성, 영양소 소화율, 혈액성상 및 사료비용에 미치는 영향", 한국축산학회지, 53(2), pp.113~118 (2011), 한국축산학회
  6. 장영달, "항생제 대체제로서 생균제가 이유자돈의 성장능력 및 영양소 이용률, 설사 빈도, 면역 반응에 미치는 영향", 한국축산학회지, 51(1), pp.25~32 (2009), 한국축산학회
  7. Jo, J. K., "Effects of exogenous enzyme supplementation to corn-and soybean meal-based or complex diets on growth performance, nutrient digestibility, and blood metabolites in growing pigs", J. Anim. Sci, 90(9), pp.3041~3048 (2012)
  8. im, B., "Effects of dietary protease on gut microbiota of weaned pigs", J. Anim. Sci, 95, pp.38~ (2017)
  9. Kim, J. S., "Bacteriophage cocktail and multi-strain probiotics in the feed for weanling pigs : effects on intestine morphology and targeted intestinal coliforms and Clostridium", Anim, 11(1), pp.45~53 (2017)
  10. Kim, D. W., "Effects of complex probiotic supplementation on growth performance, nutrient digestibility, blood metabolites, noxious gas and fecal microflora in weanling pigs", J. K. Soc. Acad-Ind. coop. Society, 21(5), pp.266~273 (2020)
  11. Lan, R. X., "Effects of Lactobacillus acidophilus supplementation in different energy and nutrient density diets on growth performance, nutrient digestibility, blood characteristics, fecal microbiota shedding, and fecal noxious gas emission in weanling pigs", Anim. Feed Sci. Technol, 219, pp.181~188 (2016)
  12. Lee, S. W., "Effect of dietary crude protein reduction in weanling pigs", Doctoral, pp.2014
  13. Lee, H. J., "Impact of supplementary microbial additives producing antimicrobial substances and digestive enzymes on growth performance, Blood metabolites and fecal microflora of weanling pigs", Anim, 11(5), pp.1217~ (2021)
  14. Neis, E. P., "The role of microbial amino acid metabolism in host metabolism", Nutr, 7(4), pp.2930~2946 (2015)
  15. NRC, "Nutrient Requirements of Swine" (2012), National. Academic. Press
  16. Olfati, Z., "Effects of gelatin as an alternative protein source and mono-component protease supplementation on growth performance, viscosity, digestibility and microbial population of ileal digesta, digestive tract traits and gut morphology of broiler chickens", Livest. Sci, 244, pp.104326~ (2021)
  17. 박상우, "Dietary protease improves growth performance, nutrient digestibility, and intestinal morphology of weaned pigs", 한국축산학회지, 62(1), pp.21~30 (2020), 한국축산학회[DOI]
  18. Portune, K. J., "Gut microbiota role in dietary protein metabolism and health-related outcomes : the two sides of the coin", Trends Food Sci. Technol, 57, pp.213~232 (2016)
  19. Rattigan, R., "Effects of reducing dietary crude protein concentration and supplementation with either laminarin or zinc oxide on the growth performance and intestinal health of newly weaned pigs", Anim. Feed Sci. Technol, 270, pp.114693~ (2020)
  20. Glenmer B. Tactacan, "Performance Responses, Nutrient Digestibility, Blood Characteristics, and Measures of Gastrointestinal Health in Weanling Pigs Fed Protease Enzyme", Animal Bioscience, 29(7), pp.998~1003 (2016), 아세아·태평양축산학회[DOI]
  21. Vondruskova, H., "Alternatives to antibiotic growth promoters in prevention of diarrhoea in weaned piglets : a review", Vet. Med, 55(5), pp.199~ (2010)
  22. Wang, Y., "Advances in low-protein diets for swine", J. Anim. Sci Biotechnol, 9(1), pp.1~14 (2018)
  23. Xiong, X., "Dietary lysozyme supplementation contributes to enhanced intestinal functions and gut microflora of piglets", Food Funct, 10(3), pp.1696~1706 (2019)
  24. Yu, J., "Dietary protease improves growth performance and nutrient digestibility in weaned piglets fed diets with different levels of soybean meal", Livest, Sci, 241, pp.104179~ (2020)
  25. Yuan, D., "Eucommia ulmoides Flavones as potential alternatives to antibiotic growth promoters in a low-protein diet improve growth performance and intestinal health in weanling piglets", Anim, 10(11), pp.1998~ (2020)
  26. Zuo, J., "Effect of dietary supplementation with protease on growth performance, nutrient digestibility, intestinal morphology, digestive enzymes and gene expression of weaned piglets", Anim. Nutr, 1(4), pp.276~282 (2015)
  27. Zhou, J., "Effect of antibiotic-free, low-protein diets with specific amino acid compositions on growth and intestinal flora in weaned pigs", Food Funct, 11(1), pp.493~507 (2020)

관련 논문AI추천

Effects of different levels of crude protein and protease on nitrogen utilization, nutrient digestibility, and growth performance in growing pigs김곡미, 김현범, 안지선, 오한진, 이지환, Jin Ho Cho, Jun Soeng Lee, Min Ho Song, Tae Heon Kim, Won Yun, Yong Ju Kim한국축산학회 · 한국축산학회지2020 · 제62권 제5호Effects of protease enzyme supplementation in weanling pigs’ diet with different crude protein levels on growth performance and nutrient digestibilityIn Ho Kim, Olivier Munezero한국축산학회 · 한국축산학회지2022 · 제64권 제5호Performance Responses, Nutrient Digestibility, Blood Characteristics, and Measures of Gastrointestinal Health in Weanling Pigs Fed Protease Enzyme김인호, Glenmer B. Tactacan, Jin H. Cho, Seung-Yeol Cho아세아·태평양축산학회 · Animal Bioscience2016 · 제29권 제7호Effect of low protein diets added with protease on growth performance, nutrient digestibility of weaned piglets and growing-finishing pigs김현범, 오한진, 이지환, Jin Ho Cho, Jun Soeng Lee, Min Ho Song, Tae Heon Kim, Won Yun, Yong Ju Kim한국축산학회 · 한국축산학회지2021 · 제63권 제3호갯벌 미생물 유래 단백질 분해 효소제의 급여가 비육돈의 생산성, 아미노산 소화율, 혈액성상, 육질특성 및 분내 휘발성 지방산과 NH3-N 함량에 미치는 영향김인호, 김해진, 민병준, 유종상, 이운교, 장정순, 조진호, 진영걸한국축산학회 · 한국축산학회지2006 · 제48권 제1호Dietary protease improves growth performance, nutrient digestibility, and intestinal morphology of weaned pigs김현범, 박상우, 송민호, Boung Mo Yang, Dong-Jun Park, Il-Hun Park, Jee-Yeon Cho, Jeong Jae Lee, Jin Ho Cho, Joowon Kang, Rider Perez-Maldonado, Sejong Oh, Soyun Kim한국축산학회 · 한국축산학회지2020 · 제62권 제1호

이 간행물의 다른 논문 (3건)

이유자돈 사료 내 Sodium Nitrite and Probiotics 첨가 수준에 따른 사양성적, 면역 반응, 장 내 미생물 및 융모 성상에 미치는 영향최요한, 김진수, 김조은, 하상훈, Joseph Moturi, Tajudeen Habeeb, 문준영pp. 57-65토마토 파우더에 대한 고지방 식이로 유도된 마우스의 비만 억제 효과장애라, 백민우, 김혜진, 정천순, 이희철pp. 66-79속보 운동시간이 제주교잡마의 혈액학 및 혈액화학치에 미치는 효과양재혁, 박용수, 정기화, 이수협pp. 80-88
원문 보기