Dietary protease improves growth performance, nutrient digestibility, and intestinal morphology of weaned pigs
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This study was conducted to evaluate effects of dietary protease (PR) on growth performance, nutrient digestibility, and intestinal morphology of weaned pigs. A total of 75 weaned pigs [7.06 ± 0.18 kg of average body weight (BW); 28 day old] were randomly allotted to 3 dietary treatments in a randomized complete block design (blocks = BW and sex): a diet based on corn and soybean meal to meet the requirement of crude protein (CP) as a positive control (PC; CP = 24.49%), a low protein diet as a negative control (NC; CP = 22.51%), and NC + 0.02% PR. The PR used in this study was a commercial product containing 75,000 protease units/g derived from Nocardiopsis prasina produced in Bacillus licheniformis. Pigs were fed the dietary treatments for 6 weeks and the diets containing 0.2% chromic oxide for the last week of this study. Blood, feces, ileal digesta, and ileum samples were collected from randomly selected two pigs in each pen on respective time points. Measurements were growth performance, apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of dry matter (DM), CP, and energy, frequency of diarrhea, packed cell volume (PCV), and ileal morphology of weaned pigs. Pigs fed PC and PR had higher (p < 0.05) final BW, average daily gain (ADG), and gain to feed ratio (G:F) during overall experimental period than those fed NC. Pigs fed PC and PR had higher (p < 0.05) AID or ATTD of DM, CP, or energy than those fed NC. Moreover, pigs fed PR had higher ratio between villus height and crypt depth (p < 0.05) and number of goblet cells (p < 0.05) than those fed NC. Addition of PR decreased (p < 0.05) frequency of diarrhea for the first two weeks after weaning compared with PC and NC. In addition, pigs fed PR had lower (p < 0.05) PCV on d 14 after weaning than those fed PC and NC. In conclusion, addition of PR in nursery diets with a low protein level significantly improved growth performance, nutrient digestibility, and intestinal morphology of weaned pigs.
Abstract
This study was conducted to evaluate effects of dietary protease (PR) on growth performance, nutrient digestibility, and intestinal morphology of weaned pigs. A total of 75 weaned pigs [7.06 ± 0.18 kg of average body weight (BW); 28 day old] were randomly allotted to 3 dietary treatments in a randomized complete block design (blocks = BW and sex): a diet based on corn and soybean meal to meet the requirement of crude protein (CP) as a positive control (PC; CP = 24.49%), a low protein diet as a negative control (NC; CP = 22.51%), and NC + 0.02% PR. The PR used in this study was a commercial product containing 75,000 protease units/g derived from Nocardiopsis prasina produced in Bacillus licheniformis. Pigs were fed the dietary treatments for 6 weeks and the diets containing 0.2% chromic oxide for the last week of this study. Blood, feces, ileal digesta, and ileum samples were collected from randomly selected two pigs in each pen on respective time points. Measurements were growth performance, apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of dry matter (DM), CP, and energy, frequency of diarrhea, packed cell volume (PCV), and ileal morphology of weaned pigs. Pigs fed PC and PR had higher (p < 0.05) final BW, average daily gain (ADG), and gain to feed ratio (G:F) during overall experimental period than those fed NC. Pigs fed PC and PR had higher (p < 0.05) AID or ATTD of DM, CP, or energy than those fed NC. Moreover, pigs fed PR had higher ratio between villus height and crypt depth (p < 0.05) and number of goblet cells (p < 0.05) than those fed NC. Addition of PR decreased (p < 0.05) frequency of diarrhea for the first two weeks after weaning compared with PC and NC. In addition, pigs fed PR had lower (p < 0.05) PCV on d 14 after weaning than those fed PC and NC. In conclusion, addition of PR in nursery diets with a low protein level significantly improved growth performance, nutrient digestibility, and intestinal morphology of weaned pigs.
참고문헌 (34)
- 김신아, "Value of palm kernel co-products in swine diets", Korean Journal of Agricultural Science, 43(5), pp.761~768 (2016), 농업과학연구소[DOI]
- National Research Council, "Nutrient requirements of swine" (2012), The National Academies Press
- Pettersson D, "Soybean: bio-active compounds", pp.288~307 (2013), IntechOpen
- DiPietro CM, "Heat inactivation of the kunitz and bowman-birk soybean protease inhibitors", J Agric Food Chem, 37, pp.39~44 (1989)
- Liener IE, "Implications of antinutritional components in soybean foods", Crit Rev Food Sci Nutr, 34, pp.31~67 (1994)
- Sklan D, "Fat digestion and absorption in chicks fed raw or heated soybean meal", J Nutr, 105, pp.57~63 (1975)
- Friedman M, "Nutritional and health benefits of soy proteins", J Agric Food Chem, 49, pp.1069~1086 (2001)
- Jo JK, "Effects of exogenous enzyme supplementation to corn- and soybean meal-based or complex diets on growth performance, nutrient digestibility, and blood metabolites in growing pigs", J Anim Sci, 90, pp.3041~3048 (2012)
- Adeola O, "Opportunities and challenges in using exogenous enzymes to improve nonruminant animal production", J Anim Sci, 89, pp.3189~3218 (2011)
- Zuo J, "Effect of dietary supplementation with protease on growth performance, nutrient digestibility, intestinal morphology, digestive enzymes and gene expression of weaned piglets", Anim Nutr, 1, pp.276~282 (2015)
- 김윤강, "Effects of dietary enzyme cocktail on growth performance, intestinal morphology, and nutrient digestibility of weaned pigs", Korean Journal of Agricultural Science, 44(4), pp.513~518 (2017), 농업과학연구소[DOI]
- Wang D, "Effects of keratinase supplementation of corn-soybean meal based diets on apparent ileal amino acid digestibility in growing pigs and serum amino acids, cytokines, immunoglobulin levels and loin muscle area in nursery pigs", Arch Anim Nutr, 65, pp.290~302 (2011)
- 민예진, "Effects of protease supplementation on growth performance, blood constituents, and carcass characteristics of growing-finishing pigs", 한국축산학회지, 61(4), pp.234~238 (2019), 한국축산학회[DOI]
- 민예진, "Effects of dietary mixture of protease and probiotics on growth performance, blood constituents, and carcass characteristics of growing-finishing pigs", 한국축산학회지, 61(5), pp.272~277 (2019), 한국축산학회[DOI]
- Prykhodko O, "Pancreatic and pancreatic-like microbial proteases accelerate gut maturation in neonatal rats", PloS ONE, 10, pp.e0116947~ (2015)
- 민예진, "Effects of Eco-friendly Multi-enzyme on Diarrhea and Immune Response of weaned Pigs", 한국유기농업학회지, 26(1), pp.151~161 (2018), 한국유기농업학회
- J. Kim, "Effects of Palm Kernel Expellers on Productive Performance, Nutrient Digestibility, and White Blood Cells of Lactating Sows", Animal Bioscience, 28(8), pp.1150~1154 (2015), 아세아·태평양축산학회[DOI]
- Rault JL, "Gas alternatives to carbon dioxide for euthanasia : a piglet perspective", J Anim Sci, 91, pp.1874~1883 (2013)
- Liu Y, "Dietary plant extracts alleviate diarrhea and alter immune responses of weaned pigs experimentally infected with a pathogenic Escherichia coli", J Anim Sci, 91, pp.5294~5306 (2013)
- 김성기, "Effects of Eco-friendly Multi-enzyme on Growth Performance, Intestinal Morphology, and Nutrient Digestibility of weaned Pigs", 한국유기농업학회지, 26(1), pp.141~149 (2018), 한국유기농업학회
- Association of Official Analytical Chemists International, "Official methods of analysis of AOAC International", AOAC International, ~ (2005)
- Williams CH, "The determination of chromic oxide in faeces samples by atomic absorption spectrophotometry", J Agric Sci, 59, pp.381~385 (1962)
- Stein HH, "Invited review : amino acid bioavailability and digestibility in pig feed ingredients : terminology and application", J Anim Sci, 85, pp.172~180 (2007)
- Choe J, "Effects of protease on growth performance and carcass characteristics of growing-finishing pigs", S Afr J Anim Sci, 47, pp.697~703 (2017)
- O’Shea CJ, "The effect of protease and xylanase enzymes on growth performance, nutrient digestibility, and manure odour in grower-finisher pigs", Anim Feed Sci Technol, 189, pp.88~97 (2014)
- Yu B, "Effects of enzyme inclusion in a maize-soybean diet on broiler performance", Anim Feed Sci Technol, 134, pp.283~294 (2007)
- L. Pan, "Effects of Coated Compound Proteases on Apparent Total Tract Digestibility of Nutrients and Apparent Ileal Digestibility of Amino Acids for Pigs", Animal Bioscience, 29(12), pp.1761~1767 (2016), 아세아·태평양축산학회[DOI]
- Hedemann MS, "Variations in enzyme activity in stomach and pancreatic tissue and digesta in piglets around weaning", Arch Anim Nutr, 58, pp.47~59 (2004)
- Lalles JP, "Gut function and dysfunction in young pigs : physiology", Anim Res, 53, pp.301~316 (2004)
- Montagne L, "A review of interactions between dietary fibre and the intestinal mucosa, and their consequences on digestive health in young non-ruminant animals", Anim Feed Sci Technol, 108, pp.95~117 (2003)
- Rooke JA, "Growth performance and gut function of piglets weaned at four weeks of age and fed protease-treated soya-bean meal", Anim Feed Sci Technol, 70, pp.175~190 (1998)
- Chen F, "Soybean-derived β-conglycinin affects proteome expression in pig intestinal cells in vivo and in vitro", J Anim Sci, 89, pp.743~753 (2011)
- Fairbrother JM, "Escherichia coli in postweaning diarrhea in pigs : an update on bacterial types, pathogenesis, and prevention strategies", Anim Health Res Rev, 6, pp.17~39 (2005)
- Heo JM, "Gastrointestinal health and function in weaned pigs : a review of feeding strategies to control post-weaning diarrhoea without using in-feed antimicrobial compounds", J Anim Physiol Anim Nutr, 97, pp.207~237 (2013)
