갯벌 미생물 유래 단백질 분해 효소제의 급여가 비육돈의 생산성, 아미노산 소화율, 혈액성상, 육질특성 및 분내 휘발성 지방산과 NH3-N 함량에 미치는 영향
Effects of Mud Flat Bacteria Origin Protease Supplementation on Growth Performance, Amino Acid Digestibility, Blood Characteristics, Meat Quality, Fecal VFA and NH3-N Concentration in Finishing Pigs
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본 연구는 비육돈 사료내 갯벌 미생물 유래 단백질 분해 효소제를 첨가하였을 때 성장률, 아미노산 소화율, 혈액성상, 육질 특성 및 분내 휘발성 지방산과 NH3-N 함량을 알아보기 위해 실시하였다. 3원 교잡종(Landrace×Yorkshire×Duroc) 비육돈 80두를 공시하였으며, 시험개시시의 체중은 60.08±2.69kg이었다. 시험설계는 기초사료내 영양소 함량과 효소제의 첨가 유뮤에 따라 high nutrient density diet 처리구, high nutrient density diet에 단백질 분해효소제를 첨가한 처리구(high nutrient density diet+0.1% protease), low nutrient density diet 처리구, low nutrient density diet 처리구에 단백질 분해효소제를 첨가한 처리구(low nutrient density diet+0.1% protease)로 4처리를 하여 처리당 5반복, 반복당 4마리씩 완전임의 배치하였다. 전체 사양시험 기간동안 일당증체량은 고 영양소 사료에 단백질 분해효소제를 첨가한 처리구가 저 영양소 사료를 급여한 처리구와 비교하여 유의적으로 증가하였다(P<0.05). DM과 N의 소화율은 고 영양소 사료를 급여한 처리구들 및 저 영양소 사료에 단백질 분해효소제를 첨가한 처리구가 저 영양소 사료를 급여한 처리구와 비교하여 유의적으로 증가하였다(P<0.05). 필수 아미노산 소화율은 저 영양소 사료에 단백질 분해효소제를 첨가한 처리구가 다른 처리구들에 비해 유의적으로 증가하였다(P<0.05). 비필수 아미노산 소화율에서는 고 영양소 사료에 단백질 분해효소제를 첨가한 처리구가 고 영양소 사료와 저 영양소 사료를 급여한 처리구들에 비해 유의적으로 증가하였다(P<0.05). BUN 함량은 고 영양소 사료에 단백질 분해효소제를 첨가한 처리구가 저 영양소 사료를 급여한 처리구와 비교하여 유의적으로 증가하였다(P<0.05). 육색에서는 명도를 나타내는 L*값은 저 영양소 사료를 급여한 처리구들이 고 영양소 사료를 급여한 처리구들과 비교하여 유의적으로 증가하였다(P<0.05). 결론적으로 비육돈 사료내 갯벌 미생물 유래 단백질 분해 효소제의 첨가는 성장률을 향상시켰고 아미노산 소화율 개선하였으며, BUN 함량과 육색에 영향을 미친 것으로 사료된다.(색인어:성장률, 아미노산 소화율, 혈액성상, 육질 특성, 휘발성 지방산, NH3-N)
Abstract
This study was conducted to evaluate effects of mud flat bacteria origin protease supplementation on growth performance, amino acid digestibility, blood characteristics, meat quality, fecal VFA (volatile fatty acids) and NH3-N (ammonia nitrogen) concentration in finishing pigs. Eighty pigs (Landrace×Yorkshire×Duroc, 60.08±2.69 kg average initial body weight) were used during experimental period. Dietary treatments included 1) high nutrient density diet, 2) high nutrient density diet+0.1% protease, 3) low nutrient density diet and 4) low nutrient density diet+0.1% protease. For overall period, ADG was improved in treatment of high nutrient density diet added protease compared with treatment of low nutrient density diet without protease (P<0.05). DM and N digestibilities were improved in treatments of high nutrient density diet and low nutrient density diet added protease compared with treatment of low nutrient density diet without protease (P<0.05). Essential amino acid digestibility was improved in treatment of low nutrient density diet added protease compared with other treatments (P<0.05). Nonessential amino acid digestibility was improved in treatment of high nutrient density diet added protease compared with treatments of high and low nutrient density diet (P<0.05). BUN (blood urea nitrogen) concentration in blood was increased in treatment of high nutrient density diet added protease compared with treatment of low nutrient density diet without protease (P<0.05). L*value of M. longissimus dorsi muscle was increased in treatments of low nutrient density diet compared with treatments of high nutrient density diet (P<0.05). In conclusion, mud flat bacteria origin protease was effective for improving growth performance, amino acid digestibility and influencing BUN concentration and meat color in finishing pigs.
참고문헌 (35)
- "Official Method of Analysis. 16th ed" (1995), Association of Afficial Analytical Chemists
- "The effect of dietary enzyme supplementation of rye- and barley-based diet on digestion and subsequent performance in weanling pigs" (1992)
- "Functional and electrophoretic characteristics of succinylated peanut flour proteins", pp.71~75 (1981)
- "Modified regents for determination of urea and ammonia" (1962)
- "The effects of dietary protein concentration on performance and visceral organ mass in finishing arrow and gilts", pp.25~27 (1996), Univ. of Nebraska. Swine Rep
- "Changes in plasma urea concentration can be used to determine protein requirement of two populations of pigs with different protein accretion rates", 73, pp.2631~ (1995)
- "Extraction of protein from defatted sesame meal using the enzyme from Bacillus sp", pp.121~1121 126 (1993)
- "Change of functional properties and extraction of sesame meal protein with phytase and protease", 30, pp.895~901 (1995)
- "Change of functional properties and extraction of protein from abolished protein resource by phytase(in Korean).", 27, pp.46~50 (1998)
- "The effect of proteolytic enzyme on the utilization of animal and plant proteins by newborn pigs and the response to predigested protein", 16, pp.568~ (1957)
- "The effects feeding conjugated linoleic acid on subsequent pork quality", 79, pp.45~ (1999)
- "Blood urea measurement as a technique for assessing protein quality" (1971)
- "Effects of long term administration of pituitary-derived bovine growth hormone and estradiol on growth in steers" (1990)
- "Determination of apparent ileal amino acid digestibility in pigs Effect of dietary amino acid level", pp.2851~2859 (1994)
- "Responses of barrows consuming a diet for- mulated on an ideal protein basis at different feeding levels", pp.30~33 (1998), Univ. of Nebraska. Swine Rep
- "The effectiveness of examining early postmortem musculature to predict ultimate pork quality" (1993)
- "Improved soy food products through food science and nutrition application(in Korean).", 29, pp.37~43 (1996)
- "Functional properties of lupin protein isolates", pp.82~86 (1985)
- "Functional properties of proteolytic enzyme-modified isolated sesame meal protein", 27, pp.708~715 (1995)
- "Purification and some characteriz- ation of the proteolytic enzyme in fruit body of Neungee", pp.276~285 (1986)
- "Enzyme supplementation of baby pig diets" (1955)
- "Characteristics of the appearance of meat Studies on the colour of meat from young bulls Journal of the Science of Food and Agriculture" (1972)
- "The diversity of proteolytic enzyme", pp.1~4 (1989), IRL Press
- "Nitrogen extractability of sesame seed and the preparation of two protein isolates", pp.565~570 (1981)
- "Procedures to Evaluate Market Hog" (1994)
- "Nutrient requirements of swine" (1998), National Academy Press
- "Studies on the properties of enzymatic hydrolysates from file fish", 28, pp.678~683 (1996)
- "Studies on the nutritional quality of rapeseed protein isolates", 12, pp.109~115 (1980)
- "CIE L* 기준에 의한 PSE육과 정상육의 육질 및 도체특성에 관한 연구", 40(6), pp.643~ (1998)
- "육성비육돈에 있어서 고-저 영양소 수준의 사료급여가 생산성에 미치는 영향", 42(5), pp.571~578 (2000)
- "생균제의 첨가가 돼지의 성장능력 및 도체특성에 미치는 효과" (1992)
- "효소제 및 생균제의 첨가가 이유자돈의 생산성과 암모니아 가스 발생에 미치는 영향", 43(4), pp.485~496 (2001)
- "비육돈 사료의 영양소 수준이 돈육 품질에 미치는 영향", 45(6), pp.1039~1046 (2003)
- "갯지렁이로부터 분리한 신규한 프로테아제", (9788) (2001)
- "고활성의 알칼리성 단백질 분해효소를 대량 생산하는 바실러스 속 I-52", (046582) (2004)
