Effects of different levels of crude protein and protease on nitrogen utilization, nutrient digestibility, and growth performance in growing pigs
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This study was conducted to evaluate the effects of different levels of crude protein (CP) and protease on nitrogen (N) utilization, nutrient digestibility, and growth performance in growing pigs. A total of six crossbred ([Landrace × Yorkshire] × Duroc) barrows were individually accepted in 1.2 m × 0.7 m × 0.96 m stainless steel metabolism cages. The pigs (average initial body weight of 27.91 ± 1.84 kg) randomly assigned to six diets with six weeks (6 × 6 Latin square design). The experiment was carried out in an environment with a temperature of 23 ± 1.5℃, a relative humidity of 83 ± 2.3% and a wind speed of 0.25 ± 0.03 m/s. The dietary treatments were arranged in a 2 × 3 factorial design with two levels of CP (15.3% or 17.1%) and three levels of protease (0 ppm, 150 ppm, or 300 ppm). The average daily gain and gain to feed ratio (G:F) tended to increase (p = 0.074) with increasing amounts of protease. The low CP level diet reduced (p < 0.050) urinary and fecal N concentrations, the total N excretion in feces, and increased (p < 0.050) N retention. Different protease levels in the diet did not affect (p > 0.05) at N intake, but supplementation of the diets with 300 ppm protease decreased (p < 0.050) the N concentration in urine and feces and tended to increase (p = 0.061) the percentage of N retention retained of the total N intake. The dietary CP level did not affect (p > 0.050) the apparent total tract digestibility (ATTD) of dry matter, digestible energy (DE), and metabolic energy (ME), but diet supplementation with 300 ppm protease showed higher (p < 0.050) ATTD of DE and ME than in the protease-free diet. Therefore, a low protein diet with protease could improve the utilization of nitrogen, thereby reducing the negative effect of N excretion into the environment while maintaining or increasing growth performance compared to a high protein diet.
Abstract
This study was conducted to evaluate the effects of different levels of crude protein (CP) and protease on nitrogen (N) utilization, nutrient digestibility, and growth performance in growing pigs. A total of six crossbred ([Landrace × Yorkshire] × Duroc) barrows were individually accepted in 1.2 m × 0.7 m × 0.96 m stainless steel metabolism cages. The pigs (average initial body weight of 27.91 ± 1.84 kg) randomly assigned to six diets with six weeks (6 × 6 Latin square design). The experiment was carried out in an environment with a temperature of 23 ± 1.5℃, a relative humidity of 83 ± 2.3% and a wind speed of 0.25 ± 0.03 m/s. The dietary treatments were arranged in a 2 × 3 factorial design with two levels of CP (15.3% or 17.1%) and three levels of protease (0 ppm, 150 ppm, or 300 ppm). The average daily gain and gain to feed ratio (G:F) tended to increase (p = 0.074) with increasing amounts of protease. The low CP level diet reduced (p < 0.050) urinary and fecal N concentrations, the total N excretion in feces, and increased (p < 0.050) N retention. Different protease levels in the diet did not affect (p > 0.05) at N intake, but supplementation of the diets with 300 ppm protease decreased (p < 0.050) the N concentration in urine and feces and tended to increase (p = 0.061) the percentage of N retention retained of the total N intake. The dietary CP level did not affect (p > 0.050) the apparent total tract digestibility (ATTD) of dry matter, digestible energy (DE), and metabolic energy (ME), but diet supplementation with 300 ppm protease showed higher (p < 0.050) ATTD of DE and ME than in the protease-free diet. Therefore, a low protein diet with protease could improve the utilization of nitrogen, thereby reducing the negative effect of N excretion into the environment while maintaining or increasing growth performance compared to a high protein diet.
참고문헌 (35)
- Vanessa T.S. Rist, "Effect of dietary protein supply originating from soybean meal or casein on the intestinal microbiota of piglets", Anaerobe, 25, pp.72~79 (2014), Elsevier BV[DOI]
- C. Lee, "Effects of metabolizable protein supply and amino acid supplementation on nitrogen utilization, milk production, and ammonia emissions from manure in dairy cows", Journal of Dairy Science, 95(9), pp.5253~5268 (2012), American Dairy Science Association[DOI]
- C. W. Holmes, "Some aspects of the energy and nitrogen metabolism of boars, gilts and barrows given diets containing different concentrations of protein", Animal Science, 31(3), pp.279~289 (1980), Cambridge University Press (CUP)[DOI]
- B J Kerr, "Effect of feeding reduced protein, amino acid-supplemented diets on nitrogen and energy balance in grower pigs.", Journal of Animal Science, 73(10), pp.3000~ (1995), Oxford University Press (OUP)[DOI]
- B. J. Kerr, "Effect on performance and carcass characteristics of nursery to finisher pigs fed reduced crude protein, amino acid-supplemented diets", Journal of Animal Science, 73(2), pp.433~440 (1995), Oxford University Press (OUP)[DOI]
- D. Deng, "Nitrogen balance in barrows fed low-protein diets supplemented with essential amino acids", Livestock Science, 109(1-3), pp.220~223 (2007), Elsevier BV[DOI]
- J. A. Hansen, "Amino acid supplementation of low-protein sorghum-soybean meal diets for 20- to 50-kilogram swine", Journal of Animal Science, 71(2), pp.442~451 (1993), Oxford University Press (OUP)[DOI]
- G. Galassi, "Effects of high fibre and low protein diets on performance, digestibility, nitrogen excretion and ammonia emission in the heavy pig", Animal Feed Science and Technology, 161(3-4), pp.140~148 (2010), Elsevier BV[DOI]
- Jianjun Zuo, "Effect of dietary supplementation with protease on growth performance, nutrient digestibility, intestinal morphology, digestive enzymes and gene expression of weaned piglets", Animal Nutrition, 1(4), pp.276~282 (2015), Elsevier BV[DOI]
- Y.-L Yin, "The effect of different carbohydrase and protease supplementation on apparent (ileal and overall) digestibility of nutrients of five hulless barley varieties in young pigs", Livestock Production Science, 71(2-3), pp.109~120 (2001), Elsevier BV[DOI]
- Yin YL, "The effect of arabinoxylanase and protease supplementation on nutritional value of diets containing wheat bran or rice bran in growing pig", J Anim Feed Sci, 13, pp.445~461 (2004)[DOI]
- F. O. Omogbenigun, "Dietary supplementation with multienzyme preparations improves nutrient utilization and growth performance in weaned pigs12", Journal of Animal Science, 82(4), pp.1053~1061 (2004), Oxford University Press (OUP)[DOI]
- F. Ji, "Effects of dietary supplementation of an enzyme blend on the ileal and fecal digestibility of nutrients in growing pigs1", Journal of Animal Science, 86(7), pp.1533~1543 (2008), Oxford University Press (OUP)[DOI]
- J. K. Jo, "Effects of exogenous enzyme supplementation to corn- and soybean meal-based or complex diets on growth performance, nutrient digestibility, and blood metabolites in growing pigs1", Journal of Animal Science, 90(9), pp.3041~3048 (2012), Oxford University Press (OUP)[DOI]
- O’Doherty JV, "The effect of protease and α-galactosidase supplementation on the nutritive value of peas for growing and finishing pigs", Ir J Agric Food Res, 38, pp.217~226 (1999)
- O. Adeola, "BOARD-INVITED REVIEW: Opportunities and challenges in using exogenous enzymes to improve nonruminant animal production", Journal of Animal Science, 89(10), pp.3189~3218 (2011), Oxford University Press (OUP)[DOI]
- P. Guggenbuhl, "Effects of dietary supplementation with a protease on the apparent ileal digestibility of the weaned piglet", Journal of Animal Science, 90(suppl_4), pp.152~154 (2012), Oxford University Press (OUP)[DOI]
- P. O. Mc Alpine, "The effect of protease and xylanase enzymes on growth performance and nutrient digestibility in finisher pigs", Journal of Animal Science, 90(suppl_4), pp.375~377 (2012), Oxford University Press (OUP)[DOI]
- National Research Council, "Nutrient requirements of swine" (1998), The National Academies Press
- G. L. Song, "Apparent ileal digestibility of amino acids and the digestible and metabolizable energy content of high-oil corn varieties and its effects on growth performance of pigs", Archives of Animal Nutrition, 57(4), pp.297~306 (2003), Informa UK Limited[DOI]
- Association of Official Analytical Chemists, "Official methods analysis" (1990), Association of Official Analytical Chemists
- P. J. Lammers, "Digestible and metabolizable energy of crude glycerol for growing pigs1,2", Journal of Animal Science, 86(3), pp.602~608 (2008), Oxford University Press (OUP)[DOI]
- J. Noblet, "Prediction of digestibility of nutrients and energy values of pig diets from chemical analysis1", Journal of Animal Science, 71(12), pp.3389~3398 (1993), Oxford University Press (OUP)[DOI]
- A. Pfeiffer, "The influence of protein intake on water balance, flow rate and apparent digestibilty of nutrients at the distal ileum in growing pigs", Livestock Production Science, 44(2), pp.179~187 (1995), Elsevier BV[DOI]
- J.M. O’Connell, "The effect of dietary crude protein level, cereal type and exogenous enzyme supplementation on nutrient digestibility, nitrogen excretion, faecal volatile fatty acid concentration and ammonia emissions from pigs", Animal Feed Science and Technology, 127(1-2), pp.73~88 (2006), Elsevier BV[DOI]
- Changsu Kong, "Effects of an enzyme complex on in vitro dry matter digestibility of feed ingredients for pigs", SpringerPlus, 4(1) (2015), Springer Science and Business Media LLC[DOI]
- D. Wang, "Effects of Keratinase on Performance, Nutrient Utilization, Intestinal Morphology, Intestinal Ecology and Inflammatory Response of Weaned Piglets Fed Diets with Different Levels of Crude Protein", Animal Bioscience, 24(12), pp.1718~1728 (2011), 아세아·태평양축산학회
- C.J. O'Shea, "The effect of protease and xylanase enzymes on growth performance, nutrient digestibility, and manure odour in grower–finisher pigs", Animal Feed Science and Technology, 189, pp.88~97 (2014), Elsevier BV[DOI]
- J.Y. DOURMAD, "Influence de l’alimentation et des performances sur les rejets azotés des porcs", INRAE Productions Animales, 7(4), pp.263~274 (1994), Universite de Bordeaux[DOI]
- T.T Canh, "Dietary protein affects nitrogen excretion and ammonia emission from slurry of growing–finishing pigs", Livestock Production Science, 56(3), pp.181~191 (1998), Elsevier BV[DOI]
- Lee P, "Dietary manipulation to reduce nitrogen excretion by pigs and its effect on performance", EAAP Publ, 69, pp.163~168 (1993)
- Sonia Y. Liu, "Strategies to enhance the performance of pigs and poultry on sorghum-based diets", Animal Feed Science and Technology, 181(1-4), pp.1~14 (2013), Elsevier BV[DOI]
- Hongyu Chen, "Impacts of energy feeds and supplemental protease on growth performance, nutrient digestibility, and gut health of pigs from 18 to 45 kg body weight", Animal Nutrition, 3(4), pp.359~365 (2017), Elsevier BV[DOI]
- Ding Wang, "Effects of keratinase supplementation of corn-soybean meal based diets on apparent ileal amino acid digestibility in growing pigs and serum amino acids, cytokines, immunoglobulin levels and loin muscle area in nursery pigs", Archives of Animal Nutrition, 65(4), pp.290~302 (2011), Informa UK Limited[DOI]
- Dick Home-jer Hou, "Structural Characteristics of Purified Glycinin from Soybeans Stored under Various Conditions", Journal of Agricultural and Food Chemistry, 52(12), pp.3792~3800 (2004), American Chemical Society (ACS)[DOI]