Effects of 4-Nonlyphenol Exposure on P. olivaceus and S. schlegeli Vitellogenesis
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The effects of the estrogenic compound 4-nonylphenol (4-NP) on vitellogenesis in juvenile olive flounder (Paralichthys olivaceus) and rockfish (Sebastes schlegeli) exposed continuously at 10, 50 and 100 ㎍ ℓ^(－1) levels for 7 days were compared. The expression of VTG mRNA level and protein using specific probes were examined. The levels of plasma estradiol-17β (E_2) and testosterone (T) were assessed by radioimmunoassay (RIA). Plasma E2 concentrations increased significantly in two female fish species exposed to 100 ㎍ ℓ^(－1) of 4-NP over concentrations in control fish. Plasma T concentrations increased in P. olivaceus. Four days after exposure, the level of VTG mRNA expression increased in P. olivaceus and S. schlegeli exposed to 20 ㎍ ℓ^(－1) of 4-NP. In addition, plasma VTG protein expression was seen in P. olivaceus and S. schlegeli. In S. schlegeli and P. olivaceus exposed to 4-NP, the changes were noticed mainly in hepatocytic vaculation after 7 days of exposure. Thus, 4-NP may disrupt vitellogenesis in immature fish both directly and indirectly via disrupted steroidogenesis and liver pathology. Immature S. schlegeli were the most sensitive to 4-NP exposure in vitellogenesis.
Abstract
The effects of the estrogenic compound 4-nonylphenol (4-NP) on vitellogenesis in juvenile olive flounder (Paralichthys olivaceus) and rockfish (Sebastes schlegeli) exposed continuously at 10, 50 and 100 ㎍ ℓ^(－1) levels for 7 days were compared. The expression of VTG mRNA level and protein using specific probes were examined. The levels of plasma estradiol-17β (E_2) and testosterone (T) were assessed by radioimmunoassay (RIA). Plasma E2 concentrations increased significantly in two female fish species exposed to 100 ㎍ ℓ^(－1) of 4-NP over concentrations in control fish. Plasma T concentrations increased in P. olivaceus. Four days after exposure, the level of VTG mRNA expression increased in P. olivaceus and S. schlegeli exposed to 20 ㎍ ℓ^(－1) of 4-NP. In addition, plasma VTG protein expression was seen in P. olivaceus and S. schlegeli. In S. schlegeli and P. olivaceus exposed to 4-NP, the changes were noticed mainly in hepatocytic vaculation after 7 days of exposure. Thus, 4-NP may disrupt vitellogenesis in immature fish both directly and indirectly via disrupted steroidogenesis and liver pathology. Immature S. schlegeli were the most sensitive to 4-NP exposure in vitellogenesis.
참고문헌 (46)
- Aida K, "Effects of castration on the smoltification of precocious male masu salmon Oncorhynchus masou", Bull Jap Soc Sci Fish, 50, pp.565~571 (1984)
- Arukwe A, "Xenobiotic and steroid biotransformation enzymes in Atlantic salmon liver treated with the estrogenic compound, 4-nonylphenol", Environ Toxicol Chem, 16, pp.2576~2583 (1997)
- Beresford N, "Issues arising when interpreting results from an in vitro assay for estrogenic activity", Toxicol Appl Pharmacol, 162, pp.22~33 (2000)
- Brion F, "Impacts of 17β-estradiol, including environmentally relevant concentrations, on reproduction after exposure during embryo-larval-, juvenile- and adult-life in zebrafish", Aquat Toxicol, 68, pp.193~217 (2004)
- Cardinali M, "Temporary impairment of reproduction in freshwater teleost exposed to nonylphenol", Rep Toxicol, 18, pp.597~604 (2004)
- Cavaco JEB, "Quantification of prolactin and PRL receptor messenger RNA in gilthead seabream after treatment with estradiol-17β", Biol Reprod, 68, pp.588~594 (2003)
- Christiansen T, "Effects of nonylphenol and 17β-oestradiol on vitellogenin synthesis, testicular structure and cytology in male eelpout Zoarcesi iparus", J Exp Biol, 201, pp.179~192 (1998)
- Denny JS, "Comparison of relative binding affinities of endocrine activity compounds to fathead minnow and rainbow trout estrogen receptors", Environ Toxicol Chem, 24, pp.2948~2953 (2005)
- Folmar LC, "Vitellogenin- induced pathology in male summer flounder", Aquat Toxicol, 51, pp.431~441 (2001)
- Gimeno S, "Feminization of young males of the common carp, Cyprinus carpio, exposed to 4-tert-pentylphenol during sexual differentiation", Aquat Toxicol, 43, pp.77~92 (1998)
- Giesy JP, "Effects of 4-nonylphenol on fecundity and biomarkers of estrogenicity in fathead minnows", Environ Toxicol Chem, 19, pp.1368~1377 (2000)
- Gray MA, "Induction of testis-ova in Japanese medaka exposed to p-nonylphenol", Environ Toxicol Chem, 16, pp.1082~1086 (1997)
- Hwang IJ, "Effects of nonylphenol and 2,2",4,6,6"-pentachlorobiphenyl on in vitro steroidogenesis in maturing oocytes of ribbed gunnel, Dictyosoma burger", Dev Rprod, 14(2), pp.143~154 (2010)
- Hern´andez-Rodr´ıguez G, "Multigenerational study of the hepatic effects exerted by the consumption of nonylphenol- and 4- octylphenol-contaminated drinking water in Sprague- Dawley rats", Environ Toxicol Pharmacol, 23, pp.73~81 (2007)
- Inui M, "Effect of UV screens and preservatives on vitellogenin and choriogenin production in male medaka", Toxicol, 194, pp.43~50 (2003)
- Jobling S, "Inhibition of testicular growth in rainbow trout exposed to alkylphenol chemicals", Environ Toxicol Chem, 15, pp.194~202 (1996)
- Jung JH, "Protein and gene expression of VTG in response to 4-nonylphenol in rockfish", Comp Biochem Physiol C, 143, pp.162~170 (2006)
- Kang IJ, "Effects of 4-nonylphenol on reproduction of Japanese medaka", Environ Toxicol Chem, 22, pp.2438~2445 (2003)
- Kannan N, "Polychlorinated biphenyls and nonylphenols in the Sea of Japan", Environ Sci Technol, 32, pp.1747~1753 (1998)
- Kim DJ, "Purification, partial characterization and immunoassay of vitellogenin from marbled sole", J Fish Sci Tech, 8(4), pp.213~219 (2006)
- Kime DE, "Endocrine Disruption in Fish", University of Sheffield Kluwer
- Korsgaard B, "Vitellogenin in Zoarces viviparus: Purification, quantification by ELISA and induction by estradiol-17 beta and 4-nonylphenol", Comp Biochemist Physiol, 120(1), pp.159~166 (1998)
- Lech JJ, "In vivo estrogenic activity of nonylphenol in rainbow trout", Fund Appl Toxicol, 30, pp.229~232 (1996)
- 김민선, "Distribution characteristics of nonylphenols in the artificial Lake Shihwa, and surrounding creeks in Korea", CHEMOSPHERE, 56, pp.783~790, PERGAMON-ELSEVIER SCIENCE LTD
- Li D, "Seasonal and spatial distribution of nonylphenol and IBP in Saemangeum Bay, Korea", Mar Poll Bull, 51, pp.966~974 (2004)
- Li Z, "Seasonal and spatial distribution of nonylphenol in Shihwa lake, Korea", Chemosphere, 56, pp.611~618 (2004)
- Lomax DP, "An enzyme linked immunosorbent assay for measuring vitellogenin in English sole: development, validation and cross-reactivity with other fleuronectids", Comp Biochem Physiol B, 121, pp.425~436 (1998)
- Luo Q, "Distinct effects of 4-nonylphenol and estrogen-17 beta on expression of estrogen receptor alpha gene in smolting sockeye salmon", Comp Biochem Physiol C, 140, pp.123~130 (2005)
- Matthews J, "Differential estrogen receptor binding of estrogenic substances: A species comparison", J Steroid Biochem Mol Biol, 74, pp.223~234 (2000)
- Mosconi G, "Gilthead seabream vitellogenin: Purification, partial characterization, and validation of an enzyme-linked immunosorbent assay", Gen Comp Endocrinol, 110, pp.252~261 (1998)
- Mollendorf A, "Cytology Cell Physiology, 3rd ed" (1973), Academic Press
- Nimrod AC, "Xenobiotic interaction with and alteration of channel catfish estrogen receptor", Toxicol Appl Pharmacol, 147, pp.381~390 (1997)
- Olsen CM, "Estrogenic effects of environmental chemicals: An interspecies comparison", Comp Biochem Physiol C, 141, pp.267~274 (2005)
- Parks LG, "Fathead minnow VTG: purification, characterization and quantitative immunoassay for the detection of estrogenic compounds", Comp Biochem Physiol C, 123, pp.113~125 (1999)
- Scholz S, "17β-ethylestradiol affects reproduction, sexual differentiation and aromatase gene expression of the medaka", Aquat Toxicol, 50, pp.363~373 (2000)
- Schwaiger J, "How estrogenic is nonylphenol? A transgenerational study using rainbow trout as a test organism", Toxicol, 59, pp.177~189 (2002)
- Soverchia L, "Modulation of vitellogenin synthesis through estrogen receptor beta-1 in goldfish juveniles exposed to 17β-estradiol and nonylphenol", Toxicol Appl Pharmacol, 209, pp.236~243 (2005)
- Sumpter JP, "Vitellogenesis as a biomarker for estrogenic contamination of the aquatic environment", Environ Health Perspect, 103(7), pp.173~178 (1995)
- Tollefsen KE, "Environmental estrogens interact with and modulate the properties of plasma sex steroid-binding proteins in juvenile Atlantic salmon", Mar Environ Res, 54, pp.697~701 (2002)
- Tollefsen, KE, "Binding of alkylphenols and alkylated non-phenolics to rainbow trout hepatic estrogen receptors", Ecotoxicol Environ Saf, 69(2), pp.163~172 (2007)
- Van den Belt K, "Comparison of vitellogenin responses in zebrafish and rainbow trout following exposure to environmental estrogens", Ecotoxicol Environ Saf, 56, pp.271~281 (2003)
- Villeneuve DL, "Effects of waterborne exposure to 4-nonylphenol on plasma sex steroid and vitellogenin concentrations in sexually mature male carp", Chemosphere, 47(1), pp.15~28 (2002)
- White R, "Environmentally persistent alkylphenolic compounds are estrogenic", Endocrinol, 135, pp.175~182 (1994)
- Yang FX, "Reproductive effects of prenatal exposure to nonylpenol on zebrafish", Comp Biochem Physiol, 142, pp.77~84 (2006)
- Yamaguchi A, "Short-term effects of endocrine-disrupting chemicals on the expression of estrogen-responsive genes in male medaka", Aquat Toxicol, 72, pp.239~249 (2005)
- Zhang LY, "Histopathological and histochemical studies on toxic effect of brodifacoum in mouse liver", Acta Acad Med Sci, 6, pp.386~388 (1984)