그물베도라치, Dictyosoma burgeri의 성숙기 난모세포스테로이드 대사에 미치는 Nonylphenol과 2,2',4,6,6'-Pentachlorobiphenyl의 효과
Effects of Nonylphenol and 2,2',4,6,6'-Pentachlorobiphenyl on In Vitro Steroidogenesis in Maturing Oocytes of Ribbed Gunnel, Dictyosoma burgeri
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초록
The in vitro effects of nonylphenol (NP) and 2,2',4,6,6'-pentachlorobiphenyl (PCB104) on ovarian steroidogenesis of the ribbed gunnel, Dictyosoma burgeri were investigated. Oocytes taken during maturation stage were incubated with 100 ng/㎖ of NP and PCB104 in the presence of exogenous precursor, [3H]-17α-hydroxyprogesterone ([3H]-17αOHP). Steroids were extracted from the media and the isolated oocytes, and the extracts were separated and identified by thin layer chromatography. The identities of the major metabolites were testosterone (T) and estradiol-17β (E2). NP treatment inhibited production of E2 metabolite in the oocytes of 1.2, 1.3 and 1.4 ㎜ although NP inhibited production of T metabolite at the oocytes of 1.1, 1.3 and 1.4 ㎜. PCB104 treatment inhibited production of T metabolite in the oocytes of all groups and E2 metabolite in the oocytes of 1.2, 1.3 and 1.4 ㎜. In conclusion, these results suggested that NP and PCB104 had an inhibitory effects on conversion of [3H]-17αOHP to T and E2 during the oocyte maturation process of ribbed gunnel.
Abstract
The in vitro effects of nonylphenol (NP) and 2,2',4,6,6'-pentachlorobiphenyl (PCB104) on ovarian steroidogenesis of the ribbed gunnel, Dictyosoma burgeri were investigated. Oocytes taken during maturation stage were incubated with 100 ng/㎖ of NP and PCB104 in the presence of exogenous precursor, [3H]-17α-hydroxyprogesterone ([3H]-17αOHP). Steroids were extracted from the media and the isolated oocytes, and the extracts were separated and identified by thin layer chromatography. The identities of the major metabolites were testosterone (T) and estradiol-17β (E2). NP treatment inhibited production of E2 metabolite in the oocytes of 1.2, 1.3 and 1.4 ㎜ although NP inhibited production of T metabolite at the oocytes of 1.1, 1.3 and 1.4 ㎜. PCB104 treatment inhibited production of T metabolite in the oocytes of all groups and E2 metabolite in the oocytes of 1.2, 1.3 and 1.4 ㎜. In conclusion, these results suggested that NP and PCB104 had an inhibitory effects on conversion of [3H]-17αOHP to T and E2 during the oocyte maturation process of ribbed gunnel.
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