Bisphenol A와 nonylphenol이 노래미, Hexagrammos agrammus 성숙기 난모세포의 스테로이드 생성과정에 미치는 영향
Effects of bisphenol A and nonylphenol on in vitro steroid production in matured oocyte of greenlings, Hexagrammos agrammus
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Endocrine disrupting chemiclas(EDCs) such as bisphenol A(BPA) and nonylphenol(NP) have estrogenic activity and can alter reproduction in fish. In the present study, the effects of BPA and NP on in vitro steroid production from oocytes of maturation stage(oocyte diameter≒1.88 mm) from the greenling(Hexagrammos agrammus) were evaluated. Oocytes were incubated with different concentrations of BPA and NP(0.1, 1, 10, 100 and 1000 ng/mL) in the presence or absence of 50 IU human chorionic gonadotropin(HCG) for 48 hours. After incubation, levels of 17α, 20β-dihydroxy- 4-pregnen-3-one(17α20βOHP), estradiol-17β(E2) and testosterone(T) from incubated media were quantified by radioimmunoassay(RIA). In BPA treatment, 100 ng/mL of BPA stimulated E2 production regardless HCG supplement. Every concentration of BPA inhibited T production without HCG although 0.1 ng/mL of BPA stimulated T production with HCG. In NP treatment, 10 ng/mL of NP stimulated 17α20βOHP and T production without HCG. 1 ng/mL of NP inhibited E2 production. Taken toghther, these results suggest that BPA might have weak estrogen-agonistic effect and NP has estrogen- antagonistic effect at final oocyte maturation stage of H. agrammus
Abstract
Endocrine disrupting chemiclas(EDCs) such as bisphenol A(BPA) and nonylphenol(NP) have estrogenic activity and can alter reproduction in fish. In the present study, the effects of BPA and NP on in vitro steroid production from oocytes of maturation stage(oocyte diameter≒1.88 mm) from the greenling(Hexagrammos agrammus) were evaluated. Oocytes were incubated with different concentrations of BPA and NP(0.1, 1, 10, 100 and 1000 ng/mL) in the presence or absence of 50 IU human chorionic gonadotropin(HCG) for 48 hours. After incubation, levels of 17α, 20β-dihydroxy- 4-pregnen-3-one(17α20βOHP), estradiol-17β(E2) and testosterone(T) from incubated media were quantified by radioimmunoassay(RIA). In BPA treatment, 100 ng/mL of BPA stimulated E2 production regardless HCG supplement. Every concentration of BPA inhibited T production without HCG although 0.1 ng/mL of BPA stimulated T production with HCG. In NP treatment, 10 ng/mL of NP stimulated 17α20βOHP and T production without HCG. 1 ng/mL of NP inhibited E2 production. Taken toghther, these results suggest that BPA might have weak estrogen-agonistic effect and NP has estrogen- antagonistic effect at final oocyte maturation stage of H. agrammus
참고문헌 (32)
- Aida K, "A review of plasma hormone changes during ovulation in cyprinid fishes", Aquaculture, 74, pp.11~21 (1988)
- Arukwe A, "Plasma levels of vitellogenin and eggshell zona radiata proteins in 4-nonylphenol and o,p'-DDT treated juvenile Atlantic salmon", Mar Environ Res, 46, pp.133~136 (1998)
- Baek HJ, "Effect of in vitro xenoestrogens on steroidogenesis in mature female fish, Chasmichthys dolichognathus", Fish Physiol Biochem, 28, pp.413~414 (2003)
- 백혜자, "Bisphenol A가 점망둑 (Chasmichthys dolichognathus)의 난소 스테로이드 호르몬 대사에 미치는 영향", 한국수산과학회지, 37(3), pp.192~196 (2004), 한국수산과학회
- Baek HJ, "Effects of BPA and DES on longchin goby (Chasmichthys dolichognathus) in vitro during the oocyte maturation", Mar Environ Res, 64, pp.79~86 (2007)
- Brotons JA, "Xenoestrogens released from lacquer coatings in food can", Environ Health Perspect, 103, pp.608~612 (1995)
- Christiansen LB, "Estrogenecity of xenobiotics in rainbow trout (Oncorhynchus mykiss) using in vivo synthesis of vitellogenin as a biomarker", Mar Environ Res, 46, pp.137~140 (1998)
- Chung EY, "On the maturity and spawning of the greenling, Hexagrammos agrammus (Temminck et Schlegel)", Korean J Ichthyol, 6(2), pp.222~236 (1994)
- Goetz FW, "Hormonal changes during meiotic maturation and ovulation in the brook trout (Salvelinus fontinalis)", Fish Physiol Biochem, 3(4), pp.203~211 (1987)
- Hwang IJ, "Estrogenic activity of nonylphenol in marine fish, Hexagrammos otakii, during oocyte development by evaluating sex steroid levels", Cybium, 32, pp.251~252 (2008)
- Jobling S, "Comparative responses of mollusks and fish to environmental estrogens and an estrogenic effluent", Aquat Toxicol, 65, pp.205~220 (2003)
- Kinnberg K, "Effects of nonylphenol and 17β-estradiol on vitellogenin synthesis and testis morphology in male platyfish, Xiphophorus maculatus", J Exp Biol, 203, pp.171~181 (2000)
- Kobayashi M, "Hormone changes during the ovulatory cycle in goldfish", Gen Comp Endocrinol, 69, pp.301~307 (1988)
- Lin YW, "In vitro estrogen modulation of pituitary and progesterone-induced oocyte maturation in Rana pipiens", J Exp Zool, 226, pp.281~291 (1983)
- Mazzoldi C, "Extended breeding season in the marbeled goby, Pomatoschistus marmoratus (Teleosti: Gobidae), in the Venctian Lagoon", Environ Biol Fish, 61, pp.175~183 (2001)
- Metcalfe CD, "Estrogenic potency of chemicals detected in sewage treatment plant effluents as determined by in vivo assays with Japanese medaka (Oryzias latipes)", Environ Toxicol Chem, 20, pp.297~308 (2001)
- Mochida K, "Effects of endocrine-disrupting chemicals on expression of ubiquitin C-terminal hydrolase mRNA in testis and brain of the Japanese common goby", Aqua Toxicol, 70, pp.123~136 (2004)
- Na OS, "Effects of bisphenol A on the hatching of fertilized eggs and spawning of adult fish in Songsari, Oryzias latipes", J Kor Fish Soc, 33, pp.378~382 (2000)
- Nagahama Y, "Regulation of oocyte maturation in fish. In: Sherwood NM and CL Hew. (ed.), Fish physiology. V13. Molecular endocrinology of fish", pp.393~439 (1994), Academic Press, London
- Nichols KM, "Effects of nonylphenol ethoxylate exposure on reproductive output and bioindicators of environmental estrogen exposure in fathead minnows Pimephales promelas", Environ Toxicol Chem, 20, pp.510~522 (2001)
- Olea N, "Estrogenecity of resin-based composites and sealants used in dentistry", Environ Health Perspect, 104, pp.298~305 (1996)
- Patiño R, "Ovarian follicle maturation and ovulation: an integrated perspective", Fish Physiol Biochem, 28, pp.305~308 (2003)
- Park MH, "In vitro effect of endocrine disrupting chemicals on oocytes maturation of yelloefin goby, Acanthogobius flavimanus" (2004), MS Thesis, Pukyong National University, Busan, Korea
- Pickford DM, "Inhibition of gonadotropin-induced oviposition and ovarian steroidogenesis in the African clawed frog (Xenopus laevis) by the pesticide methoxychlor", Aquat Toxicol, 62, pp.179~194 (2003)
- Sato N, "Release of salmon gonadotropin and 17a-hydroxyprogesterone from lipophilized gelatin emulsion at 10, 20 and 30℃ in the Japanese eel", Biotech Tech, 12, pp.797~800 (1998)
- Scott AP, "Measurement of 17α,20β-dihydroxy-4-pregnen-3-one in plasma of trout (Salmo gairdneri Richardson): Seasonal changes and response to salmon pituitary extract", Gen Comp Endocrin, 49, pp.128~134 (1983)
- Smeets JS, "Estrogenic potencies of several environmental pollutants, as determined by vitellogenin induction in a carp hepatocyte assay", Toxicol Sci, 50, pp.206~213 (1999)
- Sohoni P, "Reproductive effects of long-term exposure to bisphenol A in the fathead minnow (Pimephales promelas)", Environ Sci Tech, 35, pp.2917~2925 (2001)
- Tokumoto T, "Diethylstilbestrol induces fish oocyte maturation", Proc Natl Acad Sci, 101, pp.3686~3690 (2004)
- Van Den Belt K, "Effects of ethynylestradiol on the reproductive physiology in zebrafish (Danio rerio): time dependency and reversibility", Environ Toxicol Chem, 21, pp.767~775 (2002)
- Weber LP, "Developmental estrogenic exposure in zebrafish (Banjo rerio): II. Histological evaluation of gametogenesis and organ toxicity", Aquat Toxicol, 63, pp.431~446 (2003)
- Yoshizaki G, "The induction of oocyte maturational competence by gonadotropin in Nibe, Nibea mitsukurii (Teleostei, Sciaenidae) requires activation of the protein kinase A pathway, and RNA and protein synthesis", Fish Physiol Biochem, 25, pp.201~208 (2001)