Aluminum Inhibits Vitellogenin Production via Toxic Effects on Hepatocytes in the Rockfish Sebastes schlegelii
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Effects of aluminum (Al) on plasma vitellogenin (VTG), alkaline-labile phosphorus (ALPP), calcium (Ca), glutamate pyruvate transaminase (GPT), the hepatosomatic index (HSI), and hepatic Al concentration were examined in estradiol-17β (E_2)-administered immature rockfish Sebastes schlegeli. Fish were injected intraperitoneally with E_2 (5 mg/kg body weight [BW]) and/or Al (0, 0.1, 1, 5, and 10 mg/kg BW) and plasma and liver samples were extracted 7 days later. After sodium dodecyl sulfate polyacrylamide gel electrophoresis, the relative amount of VTG was determined by integrated optical density. VTG accounted for 23.6% of the total proteins in the control group, but this value decreased with increasing Al administration. Al reduced the concentrations of ALPP and Ca in a concentration-dependent manner and significant reduction occurred at Al concentrations greater than 5 mg/kg. The concentration of GPT increased in a concentration-dependent manner in all Al-administered rockfish. The concentrations of Al in the liver also increased, and HSI was decreased, in a concentration-dependent manner. These results suggest that Al inhibits E_2-induced VTG production by being toxic to hepatocytes in marine fish.
Abstract
Effects of aluminum (Al) on plasma vitellogenin (VTG), alkaline-labile phosphorus (ALPP), calcium (Ca), glutamate pyruvate transaminase (GPT), the hepatosomatic index (HSI), and hepatic Al concentration were examined in estradiol-17β (E_2)-administered immature rockfish Sebastes schlegeli. Fish were injected intraperitoneally with E_2 (5 mg/kg body weight [BW]) and/or Al (0, 0.1, 1, 5, and 10 mg/kg BW) and plasma and liver samples were extracted 7 days later. After sodium dodecyl sulfate polyacrylamide gel electrophoresis, the relative amount of VTG was determined by integrated optical density. VTG accounted for 23.6% of the total proteins in the control group, but this value decreased with increasing Al administration. Al reduced the concentrations of ALPP and Ca in a concentration-dependent manner and significant reduction occurred at Al concentrations greater than 5 mg/kg. The concentration of GPT increased in a concentration-dependent manner in all Al-administered rockfish. The concentrations of Al in the liver also increased, and HSI was decreased, in a concentration-dependent manner. These results suggest that Al inhibits E_2-induced VTG production by being toxic to hepatocytes in marine fish.
참고문헌 (38)
- Anan Y, "Treace element accumulation in hawksbill turtle(Eretmochelys imbricata)and green turtles(Chelonia mydas)from Yaeyama Islands,Japan", Environ Toxicol Chem, 20, pp.2802~2814 (2001)
- Björsson BT, "Distribution of calcium,magnesium and inorganic phosphate in plasma of estradiol-17β treated rainbow trout", J Comp Physiol B, 155, pp.347~352 (1985)
- Booth CE, "Effects of aluminium and low pH on net ion fluxes and ion balance in the brook trout,Salvelinus fontinalis", Can J Fish Aquat Sci, 45, pp.1563~1574 (1988)
- Bradford MM, "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteins-dye binding", Anal. Biochem, 72, pp.248~254 (1976)
- Broeg K, "The use of fish metabolic,pathological and parasitological indices in polltion monitoring.I North Sea", Helgol Mar Res, 53, pp.171~194 (1999)
- Brown DA, "Detoxification", toxification of cadmium in scorpionfish(Scorpaena guttata):acute exposure, 1984, pp.5, 93~107
- Buergel PM, "The distribution and accumulation of aluminium in rainbow trout following a wholelake alum treatment", J Freshw Ecol, 2, pp.37~44 (1983)
- Christensen LJ, "The effect of 4-nonylphenol on the synthesis of vitellogenin in the flounder,Platichthys flesus", Aquat Toxicol, 46, pp.211~219 (1999)
- De la Torre FR, "Biomarkers assessment in juvenile Cyprinus carpio exposed to waterborne cadmium", Environ Pollut, 109, pp.277~282 (2000)
- Drummond L, "Determination of phosphate.In:A Manual of Chemical and Biological Methods for Seawater Analysis", pp.22~25 (1984), Pergamon Press
- Emmersen J, "Dosage-response kinetics of serum vitellogenin,liver DNA,RNA,protein and lipid after induction by estradiol-17β in male flounders(Platichthys flesus L.)", Comp Biochem Physiol B, 63, pp.1~6 (1979)
- Exley C, "Aluminium in the brain and heart of the rainbow trout", J Fish Biol, 48, pp.706~713 (1996)
- Goyer RA, "Toxic effects of metals In: Casarett and Doull’s Toxicology: The Basic Science of Poisons. 4th ed", pp.1033~ (1991), Pergamon Press
- Haux C, "Influence of cadmium exposure on plasma calcium,vitellogenin and calcitonin in vitellogenic rainbow trout", Mar Environ Res, 24, pp.199~202 (1988)
- Heath AG, "Water Pollution and Fish Physiology" (1987), CRC Press
- Hoegh-Guldberg O, "Climate change and coral reefs:Trojan horse or false prophecy", Coral Reefs, 28, pp.569~575 (2009)
- Hwang UG, "Changes of plasma vitellogenin(VTG)and glutamate pyruvate transaminase(GPT)in the immature rockfish,Sebastes schlegeli exposed to exogenous estrogen", J Environ Toxicol, 17, pp.239~243 (2002)
- Hwang UG, "Aluminium and cadmium inhibit vitellogenin and its mRNA induction by estradiol-17β in the primary culture of hepatocytes in the rainbow trout(Oncorhynchus mykiss)", Gen Comp Endocrinol, 119, pp.69~76 (2000)
- 황운기, "4-NP가 미성숙 조피볼락, Sebastes schlegeli의 혈장 VTG, ALPP, Ca, GPT 및 HSI에 미치는 영향", 환경독성보건학회지, 22(4), pp.321~327 (2007), 환경독성보건학회
- Karlsson-Norrgren L, "Cadmium-induced changes in gill morphology of zebrafish,Brachydanio rerio(Hamilton-Buchanan),and rainbow trout,Salmo gairdneri", J Fish Biol, 27, pp.81~95 (1985)
- Kramer VJ, "Reproductive impairment and induction of alkaline-labile phosphate,a biomarker of estrogen exposure,in fathead minnows(Pimephales promelas)exposed to waterborne 17β-estradiol", Aquat Toxicol, 40, pp.335~360 (1998)
- Kwon HC, "Vitellogenin induction by estradiol-17β in primary hepatocyte culture in the rainbow trout,Oncorhynchus mykiss", Comp Biochem Physiol B, 104, pp.381~386 (1993)
- Laemmli UK, "Cleavage of structural proteins during assembly of the head of bacteriophage T4", Nature, 227, pp.680~685 (1970)
- Lee RM, "Survival and reproductive performance of the desert pupfish,Cyprinodon n.nevadensis(Eigenmann and Eigenmann),in acid water", J Fish Biol, 17, pp.507~515 (1980)
- Nagler JJ, "Serum phosphoprotein phosphorous and calcium levels as reproductive indicators of vitellogenin in highly vitellogenin mature female and estradiol-injected immature rainbow trout(Salmo gairdneri)", Can J Zool, 65, pp.2421~2425 (1987)
- Nakao W, "Aluminium inhibits protein kinase C activity in the liver without any effect on vitellogenin production in salmon(Oncorhynchus masou)", Bull Fish Sci Hokkaido Univ, 53, pp.31~36 (2002)
- Norrgren L, "Accumulation and effects of aluminium in the minnow(Phoxinus phoxinus L.)at different pH levels", J Fish Biol, 39, pp.833~847 (1991)
- Olsson PE, "Induction of metallothionein synthesis in rainbow trout,Salmo gairdneri,during long-term exposure to waterborne cadmium", Fish Physiol Biochem, 6, pp.221~229 (1989)
- Pagenkopf GK, "Gill surface interaction model for trace-metal toxicity to fishes:role of complexation,pH,and water hardness", Environ Sci Technol, 17, pp.342~347 (1983)
- Racicot JG, "Blood and liver enzymes in rainbow trout(Salmo gairdneri Rich.)with emphasis on their diagnostic use:study of CCl4,toxicity and a case of Aeromonas infection", J Fish Biol, 7, pp.825~835 (1975)
- Rask M, "Delayed spawning of perch,Perca fluviatilis L.,in acidified lakes", J Fish Biol, 36, pp.317~325 (1990)
- Runn P, "Some effects of low pH on the hatchability of eggs of perch,Perca fluviatilis L", Zoon, 5, pp.115~125 (1977)
- Tinsley D, "A comparison of plasma levels of phosphoprotein,total protein and total calcium as indirect indices of exogenous vitellogenesis in the crucian carp Carrassius carrassius", Comp Biochem Physiol B, 80, pp.913~916 (1985)
- Verbost PM, "The movement of cadmium through freswater trout branchial epithelium and its interference with calcium transport", J Exp Biol, 145, pp.185~197 (1989)
- Von Landwust C, "Fischkrankheiten in der Nordsee, Umweltbundesant Nr. 106 03 900 UBA Text 57", 96, 1996
- Wallace RA, "Studies on amphibian yolk.VII.Serum phosphoprotein synthesis by vitellogenic females and estrogen-treated males of Xenopus laevis", Can J Biochem, 46, pp.953~959 (1968)
- Wood CM, "Blood gases,acid-base status,ions,and hematology in adult brook trout(Salvelinus fontinalis)under acid", aluminum exposure, 1988, pp.45, 1575~1586
- Yasunaga G, "Trace elements accumulation in waders from Asia", Toxicol Environ Chem, 77, pp.75~92 (2000)
