남일 돌연변이 후대계통 Namil(EMS)-bl10,bph1의 도열병 및 벼멸구저항성에 대한 유전분석
Genetic Analysis on the Rice Blast and Brown planthopper Resistance of Namil(EMS)-bl10,bph1, a Japonica Rice Mutant Line
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Rice is a source of food supply as the staple food. The narrow genetic diversity of Korean Japonica cultivars is proneto be susceptible to abiotic and biotic stresses such as rice blast, bacterial blight, and brown planthopper (BPH). The bestapproach for prevention of the breeding and extermination of disease and insect pest would be the development of rice cultivarsharboring multiple disease and insect pest resistance genes. National Institute of Crop Science (NICS) in Korea developed amutant line, Namil(EMS)-bl10,bph1, by using ethyl-methane-sulfonate (EMS) as the chemical mutagen on the wild type, Namil. The mutant line exhibits strong resistance levels against rice blast and brown planthopper. To localize favorable mutated alleletypes, two mapping populations were constructed by using F2 progenies derives crosses between Namil(EMS)-bl10,bph1 andMilyang23 and Namil (wild type) and Milyang23. Each F2 progenies were evaluated in terms of DNA marker genotype as wellas agronomic traits measured, and for rice blast and BPH resistance, virulent blast isolate 94-254 and Korean biotype of BPHwere used to screen F2:3 seedlings to conduct association analysis between marker genotype and evaluated phenotype of progenylines. Through comparisons on major loci between two mapping populations, the putative major loci for rice blast resistance(RM1337, R2=54.4%) and BPH (RM0277, R2=55.1%) were primarily localized on chromosome 12.
Abstract
Rice is a source of food supply as the staple food. The narrow genetic diversity of Korean Japonica cultivars is proneto be susceptible to abiotic and biotic stresses such as rice blast, bacterial blight, and brown planthopper (BPH). The bestapproach for prevention of the breeding and extermination of disease and insect pest would be the development of rice cultivarsharboring multiple disease and insect pest resistance genes. National Institute of Crop Science (NICS) in Korea developed amutant line, Namil(EMS)-bl10,bph1, by using ethyl-methane-sulfonate (EMS) as the chemical mutagen on the wild type, Namil. The mutant line exhibits strong resistance levels against rice blast and brown planthopper. To localize favorable mutated alleletypes, two mapping populations were constructed by using F2 progenies derives crosses between Namil(EMS)-bl10,bph1 andMilyang23 and Namil (wild type) and Milyang23. Each F2 progenies were evaluated in terms of DNA marker genotype as wellas agronomic traits measured, and for rice blast and BPH resistance, virulent blast isolate 94-254 and Korean biotype of BPHwere used to screen F2:3 seedlings to conduct association analysis between marker genotype and evaluated phenotype of progenylines. Through comparisons on major loci between two mapping populations, the putative major loci for rice blast resistance(RM1337, R2=54.4%) and BPH (RM0277, R2=55.1%) were primarily localized on chromosome 12.
참고문헌 (42)
- Brar DS, "Alien introgression in rice", Plant Mol Biol, 35, pp.35~47 (1997)
- Choi HC, "A guide to rice breeding. National Institute of Crop Science", R.D.A, 107~109 (2006)
- Ha WH, "Detection of resistance gene to brown planthopper (Nilaparvata lugens Stal.) using RFLP and isozyme markers in rice", Korean J. Breed. Sci, 32, pp.319~322 (2000)
- Han SS, "Regional and annual fluctuation of race distribution of rice blast fungus (Pyricularia grisea Sacc.) during 1986~1992 in Korea", Korean J. Plant Pathol, 10, pp.25~28 (1994)
- Han SS, "Incidence of panicle blast, race distribution during 1993~1994 and pathogenicity of new races of Pyricularia grisea in Korea", Korean J. Plant Pathol, 11, pp.238~244 (1995)
- Huang CL, "Molecular evolution of the Pi-ta gene resistant to rice blast in wild rice(Oryza rufipogon)", Genetics, 179, pp.1527~1538 (2008)
- Huang Z, "Identification and mapping of two brown planthopper resistance genes in rice", Theor. Appl. Genet, 102, pp.929~934 (2001)
- Jena KK, "High-resolution mapping of a new brown planthopper (BPH) resistance gene, Bph18(t), and marker-assisted selection for BPH resistance in rice (Oryza sativa L.)", Theor. Appl. Genet, 112, pp.288~297 (2006)
- Jeung JU, "Fingerprinting template japonica and tropical indica rice genotypes by comparative analysis of DNA markers", Euphytica, 146, pp.239~251 (2005)
- Jeung JU, "Dynamics of Xanthomonas oryzae pv. oryzae populations in Korea and their relationship to known bacterial blight resistance genes", Phytopathology, 96, pp.867~875 (2006)
- Jeung JU, "A novel gene, Pi40(t), linked to the DNA markers derived from NBS-LRR motifs confers broad spectrum of blast resistance in rice", Theor. Appl. Genet, 115, pp.1163~1177 (2007)
- 정지웅, "벼 종간교잡 후대계통 ‘수원497호’의 흰잎마름병 저항성에 대한 유전분석", 한국육종학회지, 43(1), pp.81~91 (2011), 한국육종학회
- 정지웅, "야생벼 Oryza minuta에서 유래한 ‘수원506호’의 도열병 저항성유전자에 대한 유전분석", 한국육종학회지, 46(1), pp.17~27 (2014), 한국육종학회[DOI]
- Kang KH, "Development of elite lines with disease and insect resistance through wide hybridization in rice", Treat of Crop Res, 8, pp.89~104 (2007)
- Kim HY, "A semi-early maturing, high yielding and processing japonica rice cultivar “Namilbyeo”", Treat. Of Crop Res, 4, pp.141~148 (2003)
- 김병련, "연계재배법을 이용한 도열병 내구저항성 검정", 한국육종학회지, 36(5), pp.350~356 (2004), 한국육종학회
- 김동민, "벼 도열병 저항성 유전자 Pi45(t)의 균계 특이적 반응과 고밀도지도 작성", 한국육종학회지, 43(1), pp.42~49 (2011), 한국육종학회
- Kim MR, "The status of Korean rice industry and the rice processing industry", Food Industry and Nutrition, 16, pp.22~26 (2011)
- 김석만, "DNA Marker를 이용한 벼멸구 저항성 품종의 분류", 한국육종학회지, 36(5), pp.290~294 (2004), 한국육종학회
- Jae-Keun Sohn, "Identification of a Rice Gene (Bph 1) Conferring Resistance to Brown Planthopper (Nilaparvata Iugens Stal) Using STS Markers", Molecules and Cells, 20(1), pp.30~34 (2005), 한국분자세포생물학회
- Lang NT, "Genetic and physical maps of gene Bph-10 controling brown planthopper resistance in rice (Oryza sativa L.)", Omonrice, 11, pp.35~41 (2003)
- 이종희, "DNA 마커를 이용한 벼멸구 저항성 선발 및 복합내병충성 벼 계통 육성", 한국육종학회지, 43(5), pp.413~421 (2011), 한국육종학회
- McCouch SR, "Development and Mapping of 2240 New SSR Markers for Rice (Oryza sativa L.)", DNA Res, 9, pp.257~279 (2002)
- Mo YJ, "Agronomic analysis of Suweon542, a rice floury mutant line suitable for dry milling", Rice, 6, pp.37~ (2014)
- Murata K, "Bph9, a dominant brown planthopper resistance gene, is located on the long arm of rice chromosome 12", Rice Genetics Newsletter, 17, pp.84~86 (2000)
- Murray M, "Rapid isolation of high molecular weight plant DNA", Nucl. Acid. Res, 8, pp.4321~ (1980)
- in the ride cultivar ADR52, "Theor. Appl. Genet", 2012, 495~504
- Oh BG, "An anther-derived high eating quality and high yielding rice variety with resistance to bacterial leaf blight “Hwasambyeo”", Korean J. Breed. Sci, 29, pp.496~ (1997)
- Md Lutfor Rahman, "Identification of QTLs for Some Agronomic Traits in Rice Using an Introgression Line from Oryza minuta", Molecules and Cells, 24(1), pp.16~26 (2007), 한국분자세포생물학회
- Ryu JD, "Regional and annual fluctuation of race of Pyricularia oryzae during 1978-1985 in Korea", Kor. J. Plant Pathol, 3, pp.174~179 (1987)
- Sallaud C, "Identification of five new blast resistance genes in the highly blast-resistant rice variety IR64 using a QTL mapping strategy", Theor. Appl. Genet, 106, pp.794~803 (2003)
- Sharma PN, "Marker-assisted pyramiding of brown planthopper(Nilaparvata lugens Stål)resistance genes Bph1 and Bph2on rice chromosome 12", Hereditas, 140, pp.61~69 (2004)
- 심홍식, "Damage Analysis of Rice Panicle Blast on Disease Occurrence Time and Severity", The Plant Pathology Journal, 21(2), pp.87~92 (2005), 한국식물병리학회
- 심홍식, "벼 품종 및 지역에 따른 도열병균 레이스 분리와 도열병 발병정도와의 관계", 식물병 연구, 18(4), pp.324~330 (2012), 한국식물병리학회[DOI]
- Shin MS, "Genetics analysis for resistance to bacterial blight and brown planthopper in japonica rice cultivars bred in Korea", 박사, Dep. Of Agr. Bio., pp.1990
- 신문식, "벼멸구 저항성 Bph18 유전자가 벼의 수량구성요소에 미치는 영향", 한국육종학회지, 43(1), pp.56~61 (2011), 한국육종학회
- 신영섭, "Characteristics of Endosperm Starch of the Rice Mutant Lines Induced by Sodium Azide", 한국육종학회지, 41(2), pp.84~91 (2009), 한국육종학회
- Su CC, "SSR mapping of brown planthopper resistance gene Bph9in Kaharamana, an Indica rice (Oryza sativa L.)", Acata Genetica Sinica, 33, pp.262~268 (2006)
- Uhm KB, "Relationship between some weather conditions and immigration of the brown planthopper, Nilaparvata lugens Stal", Korean J. Appl. Entomol, 27, pp.200~210 (1988)
- Wang GL, "RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar", Genetics, 4, pp.1421~1434 (1994)
- Yang Q, "Identification and mapping of Pi41, a major gene conferring resistance to rice blast in the Oryza sativa subsp. indica reference cultivar, 93-11", Theor. Appl. Genet, 118, pp.1027~1034 (2009)
- Yeo US, "Linkage analysis between some agronomic traits and resistance gene to brown planthopper in rice", Korean J. Breed. Sci, 33, pp.287~293 (2001)
