유전체재해석(resequencing)에 의한 가야벼의 벼멸구 저항성 유전자 탐색 및 선발마커 개발
Detection of Genes Conferring Resistance to the Brown Planthopper (BPH) in Gayabyeo Through Genome Resequencing and Development of Their Selection Markers
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초록
Gayabyeo, a Tongil-type rice variety, has been known to be resistant to the brown planthopper (BPH) in Korea. For genetic analysis of BPH resistance of Gayabyeo, we developed an F2 and F3 population derived from a cross between Gayabyeo and Taebaegbyeo which is a Tongil-type BPH susceptible rice variety. Based on the previously detected 284,501 putative SNPs between Gayabyeo and Taebaegbyeo, 99 cleaved amplified polymorphic sequences (CAPS) markers were developed, and they have been used for genotyping 180 F2 plants. By comparison of resequencing data of Gayabyeo and the sequences of already reported BPH resistance genes (Bph3, BPH9, Bph14, BPH18, BPH26), it was revealed that Gayabyeo has Bph3 and BPH26 resistance genes. Two InDel markers, Bph3IND and BPH26IND, were developed, which can be used as selection markers in breeding program aiming at introducing BPH resistance genes of Gayabyeo into Korean high quality japonica rice varieties. In addition, BPH bioassay was performed with 180 F3 lines for BPH resistance QTL analysis. Two major QTLs were found on chromosome 4 and 12. The regions of these two QTLs included Bph3 and BPH26, which also supported that Gayabyeo has Bph3 and BPH26 resistance genes. These results would be useful in accelerating development of various BPH-resistant high quality japonica rice varieties in Korea.
Abstract
Gayabyeo, a Tongil-type rice variety, has been known to be resistant to the brown planthopper (BPH) in Korea. For genetic analysis of BPH resistance of Gayabyeo, we developed an F2 and F3 population derived from a cross between Gayabyeo and Taebaegbyeo which is a Tongil-type BPH susceptible rice variety. Based on the previously detected 284,501 putative SNPs between Gayabyeo and Taebaegbyeo, 99 cleaved amplified polymorphic sequences (CAPS) markers were developed, and they have been used for genotyping 180 F2 plants. By comparison of resequencing data of Gayabyeo and the sequences of already reported BPH resistance genes (Bph3, BPH9, Bph14, BPH18, BPH26), it was revealed that Gayabyeo has Bph3 and BPH26 resistance genes. Two InDel markers, Bph3IND and BPH26IND, were developed, which can be used as selection markers in breeding program aiming at introducing BPH resistance genes of Gayabyeo into Korean high quality japonica rice varieties. In addition, BPH bioassay was performed with 180 F3 lines for BPH resistance QTL analysis. Two major QTLs were found on chromosome 4 and 12. The regions of these two QTLs included Bph3 and BPH26, which also supported that Gayabyeo has Bph3 and BPH26 resistance genes. These results would be useful in accelerating development of various BPH-resistant high quality japonica rice varieties in Korea.
참고문헌 (27)
- Basten CJ, "QTL Cartographer: A reference manual and tutorial for QTL mapping" (1996)
- Cantindig JLA, "Planthoppers: new threats to the sustainability of intensive rice production systems in Asia", pp.191~220 (2009), International Rice Research Institute
- Du B, "Identification and characterization of Bph14, a gene conferring resistance to brown planthopper in rice", Proc Natl Acad Sci U S A, 106, pp.22163~22168 (2009)[DOI]
- Fujita D, "Rice Resistance to Planthoppers and Leafhoppers", Critical Reviews in Plant Sciences, 32, pp.162~191 (2013)[DOI]
- Hibino H, "Biology and epidemiology of rice viruses", Annu Review of Entomology, 34, pp.249~274 (1996)
- Hu J, "Pyramiding and evaluation of three dominant brown planthopper resistance genes in the elite indica rice 9311 and its hybrids", Pest Management Science, 69, pp.802~808 (2013)[DOI]
- Hu J, "Recent progress on the genetics and molecular breeding of brown planthopper resistance in rice", Rice (N Y), 9, pp.30~ (2016)[DOI]
- Huang Z, "Identification and mapping of two brown planthopper resistance genes in rice", Theor Appl Genet, 102, pp.929~934 (2001)[DOI]
- 지현소, "유전체재해석(resequencing)에 의한 통일형 벼 품종간 단일염기서열변이(SNP) 탐색", 한국육종학회지, 48(4), pp.460~469 (2016), 한국육종학회[DOI]
- Ji H, "Map-based cloning and characterization of the BPH18 gene from wild rice conferring resistance to brown planthopper (BPH) insect pest", Sci Rep, 6, pp.34376~ (2016)[DOI]
- Kim DH, "Resistance aspects on different growth stages and field resistance of rice varieties to brown planthopper, Nilaparvata lugens STAL (Homoptera : Delphacidae)", Research Report of RDA, 33, pp.33~39 (1991)
- Kim YH, "Varietal resistance of Korea rice cultivars to brown planthopper and whitebacked planthopper and their resistance sources", Research Report of RDA, 31, pp.7~13 (1989)
- Lee JO, "Study on brown planthopper biotypes. 2. Resistance mechanism of different rice varieties to Korean brown planthopper biotypes", Research Report of RDA, 27, pp.73~78 (1985)
- Lee JO, "Study on brown planthopper biotypes. 3. Plant damage of different rice cultivars to combination of biotypes", Research Report of RDA, 28, pp.52~55 (1986)
- Liu Y, "A gene cluster encoding lectin receptor kinases confers broad-spectrum and durable insect resistance in rice", Nat Biotechnol, 33, pp.301~305 (2014)
- Lorieux M, "MapDisto: fast and efficient computation of genetic linkage maps", Mol Breeding, 30, pp.1231~1235 (2012)[DOI]
- ) in the rice cultivar ADR52, "Theor Appl Genet", 2012, 495~504, 10.1007[DOI]
- Otuka A, "Planthoppers: new threats to the sustainability of intensive rice production systems in Asia", pp.221~230 (2009), International Rice Research Institute
- Park YD, "Preference, development and fecundity of the brown planthopper (Nilaparvata lugens Stal) biotypes fed on different cultivars of rice with various resistance gene", Korean Journal of Applied Entomology, 27, pp.87~93 (1988)
- Qiu Y, "Development and characterization of japonica rice lines carrying the brown planthopper-resistance genes BPH12 and BPH6", Theor Appl Genet, 124, pp.485~494 (2012)[DOI]
- Seo BY, "Planthoppers: new threats to the sustainability of intensive rice production systems in Asia", pp.303~314 (2009), International Rice Research Institute
- Bo Yoon Seo, "Electrical penetration graphic waveforms in relation to the actual positions of the stylet tips of Nilaparvata lugens in rice tissue", Journal of Asia-Pacific Entomology, 12(2), pp.89~95 (2009), 한국응용곤충학회
- Sogawa K, "The rice brown planthopper: feeding physiology and host plant interactions", Annual Review of Entomology, 27, pp.49~73 (1982)[DOI]
- Tamura Y, "Map-based cloning and characterization of a brown planthopper resistance gene BPH26 from Oryza sativa L. ssp. indica cultivar ADR52", Sci Rep, 4, pp.5872~ (2014)
- Uhm KB, "Relationship between some weather conditions and immigration of the brown planthopper, Nilaparvata lugens Stal", Koream Journal of Applied Entomology, 27, pp.200~210 (1988)
- Wilson MR, "Handbook for the identification of leafhoppers and planthoppers of rice" (1991)
- Zhao Y, "Allelic diversity in an NLR gene BPH9 enables rice to combat planthopper variation", Proc Natl Acad Sci U S A, 113, pp.12850~12855 (2016)[DOI]