남일 돌연변이 후대계통 Namil(SA)-bl5의 단간 및 도열병저항성에 대한 유전분석
Genetic Analysis on Short Culm and the Rice Blast Resistance of Namil(SA)-bl5, a Japonica Rice Mutant Line
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Rice is not only one of major crops grown in the world but also the staple food in Asia. However, in modernagriculture, the genetic diversity in rice gene pool is diminishing by selection breeding. Hence, rice is readily threatened by bioticand abiotic stresses. Rice blast is one of the major threats to stable rice production. The best approach to prevent rice blast isdevelopment of rice cultivars harboring resistance gene against blast disease. National Institute of Crop Science (NICS) in Koreadeveloped a mutant line, Namil(SA)-bl5, which has inherited short culm and rice blast resistance by treatment of sodium azide(SA). To localize favorable mutated allele types, two mapping populations were constructed by using F2 progenies derives crossesbetween Namil(SA)-bl5 and Milyang23 and Namil (wild type) and Milyang23. Each F2 progenies were evaluated in terms ofculm length and resistance level against a rice blast isolate, 94-254 along with their parental lines. Association analyses betweenmarker genotype on 68 SSR anchor markers and evaluated phenotype of progeny lines were adopted to localize the putativechromosomal locations involved to culm length and blast resistance. Association analyses localized two major loci on thechromosomes of middle-low region of 6 (R2=15.8%) and the distal region of 7 (R2=20.0%), and a major locus on the middleof chromosome 12 (R2=46.6%) for the reduced culm length and increased resistance level against rice blast, respectively.
Abstract
Rice is not only one of major crops grown in the world but also the staple food in Asia. However, in modernagriculture, the genetic diversity in rice gene pool is diminishing by selection breeding. Hence, rice is readily threatened by bioticand abiotic stresses. Rice blast is one of the major threats to stable rice production. The best approach to prevent rice blast isdevelopment of rice cultivars harboring resistance gene against blast disease. National Institute of Crop Science (NICS) in Koreadeveloped a mutant line, Namil(SA)-bl5, which has inherited short culm and rice blast resistance by treatment of sodium azide(SA). To localize favorable mutated allele types, two mapping populations were constructed by using F2 progenies derives crossesbetween Namil(SA)-bl5 and Milyang23 and Namil (wild type) and Milyang23. Each F2 progenies were evaluated in terms ofculm length and resistance level against a rice blast isolate, 94-254 along with their parental lines. Association analyses betweenmarker genotype on 68 SSR anchor markers and evaluated phenotype of progeny lines were adopted to localize the putativechromosomal locations involved to culm length and blast resistance. Association analyses localized two major loci on thechromosomes of middle-low region of 6 (R2=15.8%) and the distal region of 7 (R2=20.0%), and a major locus on the middleof chromosome 12 (R2=46.6%) for the reduced culm length and increased resistance level against rice blast, respectively.
참고문헌 (30)
- Chen Mingjiang, "Genetic analysis and fine mapping of a semi-dwarf gene in a centromeric region in rice (Oryza sativa L.)", BREEDING SCIENCE, 63(2), pp.164~168 (2013), Japanese Society of Breeding
- 조영찬, "Identification of Major Blast Resistance Genes in Korean Rice Varieties (Oryza sativa L.) Using Molecular Markers", Journal of Crop Science and Biotechnology, 10(4), pp.265~276 (2007), 한국작물학회
- Cho YG, "The semidwarf gene, sd-1, of rice (Oryza sativa L.). II. Molecular mapping and marker-assisted selection", Theor. Appl. Genet, 89, pp.54~59 (1994)
- Foster KW, "Inheritance of semidwarfism in rice, Oryza sativa L", Genetics, 88, pp.559~574 (1978)
- Han SS, "Regional and annual fluctuation of race distribution of rice blast fungus (Pyricularia grisea Sacc.) during 1986～1992 in Korea", Korean J. Plant Pathol, 10, pp.25~28 (1994)
- Han SS, "Incidence of panicle blast, race distribution during 1993～1994 and pathogenicity of new races of Pyricularia grisea in Korea", Korean J. Plant Pathol, 11, pp.238~244 (1995)
- Huang CL, "Molecular evolution of the Pi-ta gene resistant to rice blast in wild rice (Oryza rufipogon)", Genetics, 179, pp.1527~1538 (2008)
- Jena KK, "High-resolution mapping of a new brown planthopper (BPH) resistance gene, Bph18(t), and marker-assisted selection for BPH resistance in rice (Oryza sativa L.)", Theor. Appl. Genet, 112, pp.288~297 (2006)
- Jeung JU, "Fingerprinting template japonica and tropical indica rice genotypes by comparative analysis of DNA markers", Euphytica, 146, pp.239~251 (2005)
- Jeung JU, "Dynamics of Xanthomonas oryzae pv. oryzae populations in Korea and their relationship to known bacterial blight resistance genes", Phytopathology, 96, pp.867~875 (2006)
- Jeung JU, "A novel gene, Pi40(t), linked to the DNA markers derived from NBS-LRR motifs confers broad spectrum of blast resistance in rice", Theor. Appl. Genet, 115, pp.1163~1177 (2007)
- 정지웅, "벼 종간교잡 후대계통 ‘수원497호’의 흰잎마름병 저항성에 대한 유전분석", 한국육종학회지, 43(1), pp.81~91 (2011), 한국육종학회
- Kang KH, "Development of elite lines with disease and insect resistance through wide hybridization in rice", Treat of Crop Res, 8, pp.89~104 (2007)
- Kim HY, "A semi-early maturing, high yielding and processing japonica rice cultivar “Namilbyeo”", Treat. Of Crop Res, 4, pp.141~148 (2003)
- 김병련, "연계재배법을 이용한 도열병 내구저항성 검정", 한국육종학회지, 36(5), pp.350~356 (2004), 한국육종학회
- 김동민, "벼 도열병 저항성 유전자 Pi45(t)의 균계 특이적 반응과 고밀도지도 작성", 한국육종학회지, 43(1), pp.42~49 (2011), 한국육종학회
- Kim MR, "The status of Korean rice industry and the rice processing industry", Food Industry and Nutrition, 16, pp.22~26 (2011)
- 김관수, "Influence of Drought Stress on Chemical Composition of Sesame Seed", 한국작물학회지, 51(1), pp.73~80 (2006), 한국작물학회
- McCouch SR, "Development and Mapping of 2240 New SSR Markers for Rice (Oryza sativa L.)", DNA Res, 9, pp.257~279 (2002)
- Miura K, "High-resolution mapping revealed a 1.3-Mbp genomic inversion in Ssi1, a dominant semi dwarf gene in rice (Oryza sativa)", Plant Breed, 128, pp.63~69 (2009)
- Murray M, "Rapid isolation of high molecular weight plant DNA", Nucl. Acid. Res, 8, pp.4321~ (1980)
- Md Lutfor Rahman, "Identification of QTLs for Some Agronomic Traits in Rice Using an Introgression Line from Oryza minuta", Molecules and Cells, 24(1), pp.16~26 (2007), 한국분자세포생물학회
- Ryu JD, "Regional and annual fluctuation of race of Pyricularia oryzae during 1978-1985 in Korea", Kor. J. Plant Pathol, 3, pp.174~179 (1987)
- Sallaud C, "Identification of five new blast resistance genes in the highly blast-resistant rice variety IR64 using a QTL mapping strategy", Theor. Appl. Genet, 106, pp.794~803 (2003)
- 심홍식, "Damage Analysis of Rice Panicle Blast on Disease Occurrence Time and Severity", The Plant Pathology Journal, 21(2), pp.87~92 (2005), 한국식물병리학회
- 심홍식, "벼 품종 및 지역에 따른 도열병균 레이스 분리와 도열병 발병정도와의 관계", 식물병 연구, 18(4), pp.324~330 (2012), 한국식물병리학회[DOI]
- 신영섭, "Characteristics of Endosperm Starch of the Rice Mutant Lines Induced by Sodium Azide", 한국육종학회지, 41(2), pp.84~91 (2009), 한국육종학회
- Tanisaka T, "Two useful semidwarfing genes in a short-culm mutant line HS90 of rice (Oryza sativa L.)", Korean J. Breed. Sci., 44, pp.397~403 (1994)
- Wang GL, "RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar", Genetics, 4, pp.1421~1434 (1994)
- Yang Q, "Identification and mapping of Pi41, a major gene conferring resistance to rice blast in the Oryza sativa subsp. indica reference cultivar, 93-11", Theor. Appl. Genet, 118, pp.1027~1034 (2009)