국내 벼 품종의 도열병 저항성 유전자 유전형 분석
Evaluation of Molecular Markers Linked to Rice Blast Resistance Gene in Korean Rice Varieties
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초록
Rice blast is one of the most serious agricultural diseases in the world. Rice blast can be managed using low nitrogen fertilizers,treatment with chemical fungicides, and the most effective resistant varieties. Many genetic resources have been investigated and used alongwith molecular markers to breed blast-resistant rice varieties. In this study, the genetic diversity of blast resistance genes using 27 functional/linkedmarkers and rice blast incidence over three years was investigated in 296 Korean rice varieties. Blast incidence was determined using a 0-9scoring system (0=no lesions to 9=dead leaves) in nursery tests. The blast incidence of 296 rice varieties showed a significant correlationbetween years (r>0.64, p<0.001). The 261 Japonica varieties showed blast resistance compared to 114 mid-late maturing varieties or 96medium-maturing varieties. 35 Tongil-type varieties also exhibited greater resistance than Japonica varieties. DNA marker-trait association analysiswas conducted using 27 DNA markers linked to 19 blast resistance genes. Twelve DNA markers showed significant associations with theaverage blast incidence over 3 years. The ‘9871.T7E’ marker linked with Pi40 was strongly associated with blast disease, with a phenotypicvariance of 24% over 3 years (p<0.001). Among the varieties harboring the Pi40 allele, early maturing varieties accounted for 87%. Theseresults imply that the blast resistance of early maturing Korean varieties is associated with the Pi40 gene. These results will be beneficialfor breeding blast-resistant rice in Korea.
Abstract
Rice blast is one of the most serious agricultural diseases in the world. Rice blast can be managed using low nitrogen fertilizers,treatment with chemical fungicides, and the most effective resistant varieties. Many genetic resources have been investigated and used alongwith molecular markers to breed blast-resistant rice varieties. In this study, the genetic diversity of blast resistance genes using 27 functional/linkedmarkers and rice blast incidence over three years was investigated in 296 Korean rice varieties. Blast incidence was determined using a 0-9scoring system (0=no lesions to 9=dead leaves) in nursery tests. The blast incidence of 296 rice varieties showed a significant correlationbetween years (r>0.64, p<0.001). The 261 Japonica varieties showed blast resistance compared to 114 mid-late maturing varieties or 96medium-maturing varieties. 35 Tongil-type varieties also exhibited greater resistance than Japonica varieties. DNA marker-trait association analysiswas conducted using 27 DNA markers linked to 19 blast resistance genes. Twelve DNA markers showed significant associations with theaverage blast incidence over 3 years. The ‘9871.T7E’ marker linked with Pi40 was strongly associated with blast disease, with a phenotypicvariance of 24% over 3 years (p<0.001). Among the varieties harboring the Pi40 allele, early maturing varieties accounted for 87%. Theseresults imply that the blast resistance of early maturing Korean varieties is associated with the Pi40 gene. These results will be beneficialfor breeding blast-resistant rice in Korea.
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