야생벼 Oryza minuta에서 유래한 ‘수원506호’의 도열병 저항성유전자에 대한 유전분석
Genetic Analysis on the Blast Resistance Gene of ‘Suweon506’ Derived from a Wild Relative, Oryza minuta
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초록
Rice blast is one of the major threats to stable rice production. In modernized rice breeding system, development ofrice cultivars harboring resistance gene is one of the most efficient approaches against blast disease. Wild rice species, to thecontext, have been recognized as valuable genetic resources in improving resistance or tolerance level of modern commercial ricecultivars against biotic or abiotic stresses. National Institute of Crop Science (NICS), Korea developed an introgression line,‘Suweon 506’, which has inherited the rice blast resistance from a BBCC genome wild Oryza species, O. minuta (Acc. 101141). Genetic analysis was carried out to localize the chromosomal region responsible for the rice blast resistance of ‘Suweon 506’by using F2 progenies from ‘Suweon 506’ × ‘Milyang 23’. Association analysis between marker genotypes and their inferredresistance levels of F2 progenies against a Korean blast isolate, ‘93-072’ localized the target genetic region on rice chromosome12. Further association analysis with increased number of DNA markers, and e-Landings on the rice pseudomolecule 6, thesegment of ‘RM101-S10704-RM1337’ was identified to be tightly linked to the rice blast resistance gene from O. minuta at the8.8∼11.9 Mbp physical region of rice chromosome 12, where at least 9 rice blast resistance genes have been also identified. The 3.1 Mb equivalent virtual contig, composed with 31 BAC/PAC clones will be further analyzed for fine mapping and geneidentification.
Abstract
Rice blast is one of the major threats to stable rice production. In modernized rice breeding system, development ofrice cultivars harboring resistance gene is one of the most efficient approaches against blast disease. Wild rice species, to thecontext, have been recognized as valuable genetic resources in improving resistance or tolerance level of modern commercial ricecultivars against biotic or abiotic stresses. National Institute of Crop Science (NICS), Korea developed an introgression line,‘Suweon 506’, which has inherited the rice blast resistance from a BBCC genome wild Oryza species, O. minuta (Acc. 101141). Genetic analysis was carried out to localize the chromosomal region responsible for the rice blast resistance of ‘Suweon 506’by using F2 progenies from ‘Suweon 506’ × ‘Milyang 23’. Association analysis between marker genotypes and their inferredresistance levels of F2 progenies against a Korean blast isolate, ‘93-072’ localized the target genetic region on rice chromosome12. Further association analysis with increased number of DNA markers, and e-Landings on the rice pseudomolecule 6, thesegment of ‘RM101-S10704-RM1337’ was identified to be tightly linked to the rice blast resistance gene from O. minuta at the8.8∼11.9 Mbp physical region of rice chromosome 12, where at least 9 rice blast resistance genes have been also identified. The 3.1 Mb equivalent virtual contig, composed with 31 BAC/PAC clones will be further analyzed for fine mapping and geneidentification.
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