이질배수성 게놈 돌산갓의 복이배체(4n) 식물 생산
Insight on Doubled Haploid Production with an Amphidiploid Species ‘Dolsangat’ in Brassica Juncea
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Amphidiploid Brassica juncea (AABB, 2n=36) contains the synthesized genome of the diploid ancestors of Brassica rapa (AA, 2n=20) and Brassica nigra (BB, 2n=16), proven the ‘triangle of U’ model. Varieties of the B. juncea include vegetables, oilseed crops, and medicinal plants in South Asia, China, and other regions. ‘Dolsangat’, one of the cultivars of B. juncea is widely used as the main ingredient for ‘KatKimchi’, a kind of Korean traditional food Kimchi. To develop an efficient polyploidization protocol of B. juncea, we used twenty accessions. Among them, we could induce the amphidiploid plants with 0.23% in natural. A successful of polyploidization, it is essential of chromosome doubling regent treatment of B. juncea. At first, we tried to colchicine treatment in the embryo stage and it was very harmful to the embryo and could get few plants. The second, we made the regeneration plants from embryo to rooting phase and shocked them in 0.34% colchicine contained distilled water. We could induce amphidiploid plants with a success rate of 63.4%. Also, we surveyed glucosinolate content and JB1, Alsami, and JD6 showed high total contents. These plants will use for genetic materials for breeding, genetic and molecular studies.
Abstract
Amphidiploid Brassica juncea (AABB, 2n=36) contains the synthesized genome of the diploid ancestors of Brassica rapa (AA, 2n=20) and Brassica nigra (BB, 2n=16), proven the ‘triangle of U’ model. Varieties of the B. juncea include vegetables, oilseed crops, and medicinal plants in South Asia, China, and other regions. ‘Dolsangat’, one of the cultivars of B. juncea is widely used as the main ingredient for ‘KatKimchi’, a kind of Korean traditional food Kimchi. To develop an efficient polyploidization protocol of B. juncea, we used twenty accessions. Among them, we could induce the amphidiploid plants with 0.23% in natural. A successful of polyploidization, it is essential of chromosome doubling regent treatment of B. juncea. At first, we tried to colchicine treatment in the embryo stage and it was very harmful to the embryo and could get few plants. The second, we made the regeneration plants from embryo to rooting phase and shocked them in 0.34% colchicine contained distilled water. We could induce amphidiploid plants with a success rate of 63.4%. Also, we surveyed glucosinolate content and JB1, Alsami, and JD6 showed high total contents. These plants will use for genetic materials for breeding, genetic and molecular studies.
참고문헌 (27)
- Baillie AMR, "In vitro culture of isolated microspores and regeneration of plants in Brassica campestris", Plant Cell Rep, 11, pp.234~237 (1992)
- Bhatia R. Dey SS, "Modification of important factors for efficient microspore embryogenesis and doubled haploid production in field grown white cabbage(Brassica oleracea var. capitate L. )genotypes in India", Sci Hortic, 233, pp.178~187 (2018)
- Burnett L, "Embryogenesis and plant regeneration from isolated microspores of Brassica rapa L ssp. oleifera", Plant Cell Rep, 11, pp.215~218 (1992)
- Cao MQ, "Embryogenesis and plant regeneration of pakchoi(Brassica rapa L. ssp. chinensis)via in vitro isolated microspore culture", Plant Cell Rep, 13, pp.447~450 (1994)
- Cartea ME, "Phenolic compounds in brassica vegetables", Moleules, 16, pp.251~280 (2010)
- Eng W-H, "Polyploidization using colchicine in horticultural plants : A reiview", Sci Hortic, 246, pp.604~617 (2019)
- Gantait S, "Induction and identification of tetraploids using in vitro colchicine treatment of Gerbera jamesonii Bolus cv. Sciella", Plant Cell Tiss Organ Cult, 106, pp.485~493 (2011)
- Joosen R, "Combined transcriptome and proteome analysis identifies pathways and robust markers associated with the establishment of Brassica napus microspore-derived embryo development", Plant Physiol, 144, pp.155~172 (2007)
- Unraveling the molecular development platform using Comparative genomics in Brassicaceae, "Kim JS", National Institute of Agricultural Sciences, ~, pp.PJ010157 (2017)
- Lee SS, "Effects of cultural vessel, plant growth regulator, illuminating and shaking on embryo induction and growth in microspore culture of heading Chinese cabbage", J Kor Soc Hort Sci, 41, pp.16~20 (2000)
- Lichter R, "Induction of haploid plants from isolated pollen of Brassica napus", Z. Pflanzenphysiol, 105, pp.427~434 (1982)
- Lionneton E, "Improved microspore culture and doubled-haploid plant regeneration in the brown condiment mustard(Brassica juncea)", Plant Cell Rep, 20, pp.126~130 (2001)
- Möllers C, "Efficient production of doubled haploid Brassica napus plants by colchicine treatment of microspore", Euphytica, 75, pp.95~104 (1994)
- Murashige T, "A revised medium for rapid growth and bio-assays with tobacco tissue culture", Physiol Plant, 15, pp.473~497 (1962)
- Prem D, "Activated charcoal induced high frequency microspore embryogenesis and efficient doubled haploid production in Brassica juncea", Plant Cell Tiss Organ Cult, 93, pp.269~282 (2008)
- Seo M, "Understanding of MYB transcription factors involved in glucosinolate biosynthesis in Brassicaceae", Moleules (2017)
- 서미숙, "배추 종간 잡종의 소포자배양에 의한 Double haploid 집단의 플라보노이드 함량 분석", Journal of Plant Biotechnology, 44(1), pp.35~41 (2017), 한국식물생명공학회
- Seo MS, "Expression profiles of BrMYB transcription factors related to glucosinolate biosynthesis and stress response in eight subspecies of Brassica rapa", FEBBS Open Bio, 7, pp.1646~1659 (2017)
- 서미숙, "기내배양을 통한 Brassica 작물의 반수체 생산에 관한 연구 동향", 한국국제농업개발학회지, 27(4), pp.522~528 (2015), 한국국제농업개발학회[DOI]
- 서미숙, "배추 유전자원의 소포자 유래 배 발생 효율에 미치는 배양 조건 구명", Journal of Plant Biotechnology, 41(3), pp.116~122 (2014), 한국식물생명공학회
- Takahata Y, "High frequency embryo-genesis and plant regeneration in isolated microspore culture of Brassica oleracea L", Plant Sci, 74, pp.235~242 (1991)
- Tuncer B, "Effect of colchicine and High temperature treatments on isolated microspore culture in various cabbage(Brassica oleracea)types. Int", J Agric Bio, 13, pp.819~822 (2011)
- Tuncer B, "Effect of heat shock treatment on microspore embryogenesis in Brassica oleracea Species. Tar. Bilim. Derg", J Agric Sci, 22, pp.548~554 (2016)
- Wei Z, "The culture of isolated microspores of ornamental kale (Brassica oleracea var. acephala) and the importance of genotype to embyo regeneration", Sci Hortic, 117, pp.69~72 (2008)
- Zaki M, "1995 Modification of cell development in vitro : The effect of colchicine on anther and isolated microspore culture in Brassica napus", Plant Cell Tiss Organ Cult, 40, pp.255~270 (1995)
- Zhang W, "The culture of isolated microspores of ornamental kale(Brassica oleracea var. acephala)and the importance of genotype to embryo regeneration", Sci Hortic, 117, pp.69~72 (2008)
- Zhou HW, "In vitro induction of tetraploids in cassava variety ‘Xinxuan 048’ using colchicine", Plant Cell Tiss Organ Cult, 128, pp.723~729 (2017)