본문 바로가기
KERD
논문KCI 등재

유채 잡종 1세대의 소포자 배양에 의한 배가반수체 집단 선발 및 지방산 조성 분석

Production of doubled haploid population derived from the microspore culture of rapeseed (Brassica napus L.) F1 generation and analysis of fatty acid composition

김광수|박주현|안다희|차영록|이지은
한국식물생명공학회 (Korean Society for Plant Biotechnology)|2022.03|Journal of Plant Biotechnology|제49권 제1호|pp. 74-81|DOI 10.5010/JPB.2022.49.1.074

2

조회

0

다운로드

1

인용

0

참고문헌

키워드

Fatty acid compositionRapeseedMicrospore cultureDoubled haploidPloidy
주제분류농학

초록

Brassica napus, an oil crop that produces rapeseed oil, is an allotetraploid (AACC, 2n = 38) produced by natural hybridization between B. rapa and B. oleracea. In this study, microspore was cultured using the F1 developed from a cross between ‘EMS26’ line with high oleic acid content and ‘J8634-B-30’ lines. The flower bud size showing the nuclear development at the late uninucleate and binucleate stage with high embryogenesis rate was 2.6 ~ 3.5 mm. Microspores were cultured using only this size and after then most microspore embryo developed into secondary embryos and then regeneration plants obtained from the developed multilobe. The analysis of the ploidy of the plants revealed that 66.7% and 27.8% of the total lines were tetraploids and octoploids, respectively. The sizes of stomatal cells in tetraploids, octoploids, and diploids were 25.5, 35.6, and 19.9 μm, respectively, indicating that ploidy level was positively correlated with cell size. Furthermore, 62 tetraploid doubled haploid (DH) lines were selected. The average oleic acid (C18:1) and linolenic acid (C18:3) concentrations of DH were 72.3% and 6.2%, respectively. Oleic acid and linolenic acid concentrations exceeded the two parental values in 5 and 14 DH lines, respectively, suggesting that these two fatty acids had transgressive segregation. Therefore, the DH population can be utilized for the biosynthesis of unsaturated fatty acids in rapeseed and related genes. It can also be used as a breeding material for varieties with high oleic acid concentrations.

Abstract

Brassica napus, an oil crop that produces rapeseed oil, is an allotetraploid (AACC, 2n = 38) produced by natural hybridization between B. rapa and B. oleracea. In this study, microspore was cultured using the F1 developed from a cross between ‘EMS26’ line with high oleic acid content and ‘J8634-B-30’ lines. The flower bud size showing the nuclear development at the late uninucleate and binucleate stage with high embryogenesis rate was 2.6 ~ 3.5 mm. Microspores were cultured using only this size and after then most microspore embryo developed into secondary embryos and then regeneration plants obtained from the developed multilobe. The analysis of the ploidy of the plants revealed that 66.7% and 27.8% of the total lines were tetraploids and octoploids, respectively. The sizes of stomatal cells in tetraploids, octoploids, and diploids were 25.5, 35.6, and 19.9 μm, respectively, indicating that ploidy level was positively correlated with cell size. Furthermore, 62 tetraploid doubled haploid (DH) lines were selected. The average oleic acid (C18:1) and linolenic acid (C18:3) concentrations of DH were 72.3% and 6.2%, respectively. Oleic acid and linolenic acid concentrations exceeded the two parental values in 5 and 14 DH lines, respectively, suggesting that these two fatty acids had transgressive segregation. Therefore, the DH population can be utilized for the biosynthesis of unsaturated fatty acids in rapeseed and related genes. It can also be used as a breeding material for varieties with high oleic acid concentrations.

참고문헌 (38)

  1. Atri C, "A protocol for flow cytometric determination of expected chromosome number of Brassica juncea L. introgression lines", Journal of Oilseed Brassica, 1(2), pp.170~174 (2016)
  2. Browse J, "Altering the Fatty Acid Composition of Vegetable Oils" (1998), Cambridge University Press
  3. Chalhoub B, "Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome", Science, 345(6199), pp.950~953 (2014)
  4. Chang NW, "Effects of the ratio of polyunsaturated and monounsaturated fatty acid to saturated fatty acid on rat plasma and liver lipid concentrations", Lipids, 33(5), pp.481~487 (1998)
  5. Datta SK, "Current trends in the embryology of angiosperms", pp.471~488 (2001), Springer
  6. Forster BP, "Doubled haploids in genetics and plant breeding", Plant Breed Rev, 25, pp.57~88 (2005)
  7. Gacek K, "Genome-wide association study of genetic control of seed fatty acid biosynthesis in Brassica napus", Frontiers in Plant Science, 7, pp.2062~ (2017)
  8. Guha S, "In vitro production of embryos from anthers of Datura", Nature, 204(4957), pp.497~497 (1964)
  9. Höfer M, "In vitro androgenesis in apple-improvement of the induction phase", Plant Cell Reports, 22(6), pp.365~370 (2004)
  10. Hu X, "Mapping of the loci controlling oleic and linolenic acid contents and development of fad2 and fad3 allele-specificmarkers in canola (Brassica napus L.)", Theoretical and Applied Genetics, 113(3), pp.497~507 (2006)
  11. Jang YS, "Comparisons of developmental stages of microspore by bud size and embryogenesis from its microspore in Brassica species", Korean J Breed, 29(4), pp.480~485 (1997)
  12. Javidfar F, "Identification of molecular markers associated with oleic and linolenic acid in spring oilseed rape(Brassica napus)", Plant Breeding, 125(1), pp.65~71 (2006)
  13. Keller W, "Stimulation of embryogenesis and haploid production in Brassica campestris anther cultures by elevated temperature treatments", Theoretical and Applied Genetics, 55(2), pp.65~67 (1979)
  14. 김정선, "이질배수성 게놈 돌산갓의 복이배체(4n) 식물 생산", 한국육종학회지, 51(4), pp.341~350 (2019), 한국육종학회[DOI]
  15. 김광수, "국내 육성 품종 ‘탐미유채’의 소포자 배양 시 배양조건이 배발생에 미치는 영향", 한국작물학회지, 57(4), pp.317~323 (2012), 한국작물학회[DOI]
  16. 김광수, "양성자 및 감마선을 처리한 유채 M₂세대의 소포자로부터 반수체 배발생", 한국작물학회지, 53(2), pp.150~155 (2008), 한국작물학회
  17. Kyo M, "Studies on conditions for cell division and embryogenesis in isolated pollen culture of Nicotiana rustica", Plant physiology, 79(1), pp.90~94 (1985)
  18. 이태성, "파종시기에 따른 유채(Brassica napus L.) 기름함량 및 지방산 조성 특성", 한국자원식물학회지, 27(2), pp.202~208 (2014), 한국자원식물학회[DOI]
  19. Licher R, "Induction of haploid plants from isolated pollen of Brassica napus", Zeitschrift für Pflanzenphysiologie, 105(5), pp.427~434 (1982)
  20. Long W, "Identification and functional analysis of two new mutant BnFAD2 alleles that confer elevated oleic acid content in rapeseed", Frontiers in Genetics, 9, pp.399~ (2018)
  21. Mohammadi PP, "Doubled haploid plants following colchicine treatment of microspore-derived embryos of oilseed rape (Brassica napus L.)", Plant Cell, Tissue and Organ Culture (PCTOC), 108(2), pp.251~256 (2012)
  22. Murashige T, "A revised medium for rapid growth and bioassays with tobacco tissue cultures", Physiol Plant, 15, pp.473~497 (1962)
  23. 나해영, "The Effects of Plant Growth Regulators, Activated Charcoal, and AgNO3 on Microspore Derived Embryo Formation in Broccoli (Brassica oleracea L. var. italica)", Horticulture, Environment, and Biotechnology, 52(5), pp.524~529 (2011), 한국원예학회
  24. Nakata K, "Differentiation of embryoids from developing germ cells in anther culture of tobacco", Japanese Journal of Genetics, 43(1), pp.65~70 (1968)
  25. Ouyang TW, "Induction of pollen plants from anthers of Triticum aestivum L. cultured in vitro", Scientia Sinica, 16(1), pp.79~90 (1973)
  26. Park YJ, "Comparison of frequency embryogenesis through microspore culture of domestic cultivars in Brassica napus L", Korean Journal of Crop Science, 51(1), pp.237~241 (2006)
  27. Przybylski R, "Performance of regular and modified canola and soybean oils in rotational frying", Journal of the American Oil Chemists’ Society, 90(9), pp.1271~1280 (2013)
  28. Scheffler J, "Desaturase multigene families of Brassica napus arose through genome duplication", Theoretical and Applied Genetics, 94(5), pp.583~591 (1997)
  29. 서미숙, "배추 유전자원의 소포자 유래 배 발생 효율에 미치는 배양 조건 구명", Journal of Plant Biotechnology, 41(3), pp.116~122 (2014), 한국식물생명공학회
  30. Shumilina D, "Effects of genotype and culture conditions on microspore embryogenesis and plant regeneration in Brassica Rapa ssp", Rapa L. Plants, 9(2), pp.278~ (2020)
  31. Siebel J, "A comparison of anther and microspore culture as a breeding tool in Brassica napus", Theoretical and Applied Genetics, 78(4), pp.473~479 (1989)
  32. Tatum T, "Genome size variation in pumpkin (Cucurbita sp.)", Annals of applied biology, 149(2), pp.145~151 (2006)
  33. Thomas E, "Embryogenesis from microspores of Brassica napus", Z Pflanzenzüchtg, 74, pp.77~81 (1975)
  34. Thomson JA, "Clarification of the taxonomic status and relationships of Pteridium caudatum(Dennstaedtiaceae)in Central and South America", Botanical Journal of the Linnean Society, 140(3), pp.237~248 (2002)
  35. Weber S, "Improved doubled haploid production protocol for Brassica napus using microspore colchicine treatment in vitro and ploidy determination by flow cytometry", Plant Breeding, 124(5), pp.511~513 (2005)
  36. WHO, "Diet, nutrition and the prevention of chronic diseases", World Health Organ Tech Rep Ser, 916(i-viii), pp.1~149 (2003)
  37. Xing Ying Yan, "Mapping of QTLs controlling content of fatty acid composition in rapeseed (Brassica napus)", Genes & Genomics, 33(4), pp.365~371 (2011), 한국유전학회
  38. Yang Q, "Identification of FAD2 and FAD3 genes in Brassica napus genome and development of allele-specific markers for high oleic and low linolenic acid contents", Theoretical and Applied Genetics, 125(4), pp.715~729 (2012)

관련 논문AI추천

갓 소포자 배양에 의한 Doubled haploid 집단 육성 및 표현형 분석구윤숙, 김명희, 김정선, 문미선, 박현우, 이나형한국육종학회 · 한국육종학회지2024 · 제56권 제3호배추 유전자원의 소포자 유래 배 발생 효율에 미치는 배양 조건 구명고호철, 박범석, 서미숙, 손성한, 진민아한국식물생명공학회 · Journal of Plant Biotechnology2014 · 제41권 제3호배추 자원별 유전자형 변이가 배추 소포자배양 효율성에 미치는 영향김진희, 김태복, 도경란, 배솔희, 서태철, 위승환, 윤효인, 이혜진한국육종학회 · 한국육종학회지2024 · 제56권 제4호배추 종간 잡종의 소포자배양에 의한 Double haploid 집단의 플라보노이드 함량 분석강상호, 김정선, 서미숙, 원소윤한국식물생명공학회 · Journal of Plant Biotechnology2017 · 제44권 제1호이질배수성 게놈 돌산갓의 복이배체(4n) 식물 생산권수진, 김정선, 문미선, 서미숙, 원소윤한국육종학회 · 한국육종학회지2019 · 제51권 제4호소포자 배양의 시기와 조건이 양배추의 배발생에 미치는 효과박민영, 박수형, 이정수, 임용표, 장하영한국식물생명공학회 · Journal of Plant Biotechnology2017 · 제44권 제1호

이 간행물의 다른 논문 (11건)

Erratum : 인공조명이 수경재배 새싹보리(Hordeum vulgare L.)의 생장과 품질에 미치는 영향김주성pp. 1-1Erratum : 진보된 DNA barcoding 기술을 이용한 당귀(Angelica)속 식물의 기원 판별 기술에 관한 연구 동향이신우, 김윤희, 신용욱pp. 2-2Botanical features and ethnopharmacological potential of Leonotis nepetifolia (L.) R. Br: a reviewRoggers Gang, 강영민pp. 3-14Genome-wide analysis of Solanum lycopersicum L. cyclophilins이도진, Khadiza Khatun, Arif Hasan Khan Robin, Md Rafiqul Islam, Subroto Das Jyoti, 정미영, 김창길pp. 15-29엽록체 전장유전체 비교를 통한 PCR 기반의 Solanum brevicaule 특이적 분자마커 개발박태호pp. 30-38
원문 보기