배추 유전자원의 소포자 유래 배 발생 효율에 미치는 배양 조건 구명
Efficiency of microspore embryogenesis in Brassica rapa using different genotypes and culture conditions
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참고문헌
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초록
Total of fifty accessions of Brassica rapa withvarious morphological characteristics were used for productionof double haploid plants though microspore culture inBrassica rapa. Among them, only 30 accessions inducedembryos from microspores. The highest efficiency of embryoinduction of 1.194 per bud was obtained from IT135449 ofturnip type, while 3 accessions of sarson (winter oil) type didnot generate embryo. The effect of heat shock periods forembryogenesis was also investigated with 4 accessions(IT135449; Turnip type, IT199710; Chinese cabbage type,IT212886; Pak choi type, IT218043; Summer oil type). Thehigh productions of embryos were observed in IT135449,IT199710 and IT212886 when microspores were pre-culturedto 32˚C for 2 days. In IT218043, high embryogenesis wasobserved at the 3 days of heat shock treatment. The optimalcondition of shoot regeneration for IT199710 was observedin MS medium supplemented with NAA 0.5 mg·L-1 andBAP 1 mg·L-1. In contrast, the IT135449 and IT212886 wereobserved high regeneration frequency in MS medium withoutplant growth regulators. All the plantlets regenerated frommicrospore-derived embryos have been successfully transplantedto soil, and bud self-pollinated seeds were produced fromdoubled haploid plants. This indicated that double-haploidgenotype was likely generated naturally during embryogenesisprocess.
Abstract
Total of fifty accessions of Brassica rapa withvarious morphological characteristics were used for productionof double haploid plants though microspore culture inBrassica rapa. Among them, only 30 accessions inducedembryos from microspores. The highest efficiency of embryoinduction of 1.194 per bud was obtained from IT135449 ofturnip type, while 3 accessions of sarson (winter oil) type didnot generate embryo. The effect of heat shock periods forembryogenesis was also investigated with 4 accessions(IT135449; Turnip type, IT199710; Chinese cabbage type,IT212886; Pak choi type, IT218043; Summer oil type). Thehigh productions of embryos were observed in IT135449,IT199710 and IT212886 when microspores were pre-culturedto 32˚C for 2 days. In IT218043, high embryogenesis wasobserved at the 3 days of heat shock treatment. The optimalcondition of shoot regeneration for IT199710 was observedin MS medium supplemented with NAA 0.5 mg·L-1 andBAP 1 mg·L-1. In contrast, the IT135449 and IT212886 wereobserved high regeneration frequency in MS medium withoutplant growth regulators. All the plantlets regenerated frommicrospore-derived embryos have been successfully transplantedto soil, and bud self-pollinated seeds were produced fromdoubled haploid plants. This indicated that double-haploidgenotype was likely generated naturally during embryogenesisprocess.
참고문헌 (27)
- Baillie AMR, "In vitro culture of isolated microspores and regeneration of plants in Brassica campestris", Plant Cell Rep, 11, pp.234~237 (1992)
- Blanca J, "Transcriptome sequencing for SNP discovery across Cucumis melo", BMC Genomics, 13, pp.280~ (2012)
- Cao MQ, "Embryogenesis and plant regeneration of pakchoi (Brassica rapa L. ssp. chinensis) via in vitro isolated microspore culture", Plant Cell Rep, 13, pp.447~450 (1994)
- Cistué L, "Production of doubled haploids in durum wheat (Triticum turgidum L.) through isolated microspore culture", Plant Cell Rep, 25, pp.257~264 (2006)
- Dias JCD, "Effect of incubation temperature regimes and culture medium on broccoli microspore culture embryogenesis", Euphytica, 119, pp.389~394 (2001)
- Dubas E, "Endogenous ABA concentration and cytoplasmic membrane fluidity in microspores of oilseed rape (Brassica napus L.)genotypes differing in responsiveness to androgenesis induction", Plant Cell Rep (2013)[DOI]
- Fabijanski SF, "Heat shock response during anther culture of broccoli (Brassica oleracea var. italica)", Plant Cell Tissue Org Cult, 26, pp.203~212 (1991)
- Ferrie AMR, "Evaluation of Brassica rapa L. genotypes for microspore culture response and identification of a highly embryogenic line", Plant Cell Rep, 14, pp.580~584 (1995)
- Gamborg OL, "Nutrient requirements of suspension cultures of soybean root cells", Ept. Cell Res, 50, pp.151~158 (1968)
- Gu HH, "Cold pretreatment enhances microspore embryogenesis in oilseed rape (Brassica napus L.)", Plant Growth Regulation, 42, pp.137~143 (2004)
- 조만현, "중국도입 배추 소포자배양에 의한 배가반수체의 종자생산능력", 원예과학기술지, 30(5), pp.573~578 (2012), 한국원예학회[DOI]
- Joosen R, "Combined transcriptome and proteome analysis identifies pathways and robust markers associated with the establishment of Brassica napus microspore-derived embryo development", Plant Physiol, 144, pp.155~172 (2007)
- Kim YH, "Microspore culture of chinese cabbage (Brassica campestris ssp. pekinensis) and Korean turnip (B. campestris ssp. rapa)", J Kor Soc Hort Sci, 38, pp.368~371 (1997)
- 김광수, "국내 육성 품종 ‘탐미유채’의 소포자 배양 시 배양조건이 배발생에 미치는 영향", 한국작물학회지, 57(4), pp.317~323 (2012), 한국작물학회[DOI]
- Lee SS, "Effects of cultural vessel, plant growth regulator, illuminating and shaking on embryo induction and growth in microspore culture of heading chinese cabbage", J Kor Soc Hort Sci, 41, pp.16~20 (2000)
- Lee SS, "Developing stable progenies of xBrassicoraphanus, an intergeneric allopolyploid between Brassica rapa and Raphanus sativus, through induced mutation using microspore culture", Theor Appl Genet, 122, pp.885~891 (2011)
- Lichter R, "Induction of haploid plants from isolated pollen of Brassica napus Z", Pflanzenphysiol, 105, pp.427~434 (1982)
- Murashige T, "A revised medium for rapid growth and bio-assays with tobacco tissue cultures", Physiol Plant, 15, pp.473~497 (1962)
- 나해영, "The Effects of Plant Growth Regulators, Activated Charcoal, and AgNO3 on Microspore Derived Embryo Formation in Broccoli (Brassica oleracea L. var. italica)", Horticulture, Environment, and Biotechnology, 52(5), pp.524~529 (2011), 한국원예학회
- Pathirana R, "Haploid and doubled haploid plants from developing male and female gametes of Gentiana triflora", Plant Cell Rep, 30, pp.1055~1065 (2011)
- Pink D, "Double haploids, markers and QTL analysis in vegetable Brassicas", Euphytica, 164(2), pp.509~514 (2008)
- Takahata Y, "High frequency embryo-genesis and plant regeneration in isolated microspore culture of Brassica oleracea L", Plant Sci, 74, pp.235~242 (1991)
- Wang XW, "The genome of the mesopolyploid crop species Brassica rapa", Nat Genet, 43, pp.1035~U1157 (2011)
- Wei Z, "The culture of isolated microspores of ornamental kale (Brassica oleracea var. acephala) and the importance of genotype to embyo regeneration", Scientia Horticul, 117, pp.69~72 (2008)
- Zhao J, "Design of a Brassica rapa core collection for association mapping studies", Genome, 53, pp.884~898 (2010)
- Zhao J, "Genetic relationships within Brassica rapa as inferred from AFLP fingerprints", Theor Appl Genet, 110, pp.1301~13041 (2005)
- Zou Z, "QTL analysis using SNP markers developed by next-generation sequencing for identification of candidate genes controlling 4-methylthio-3-butenyl glucosinolate contents in roots of radish, Raphanus sativus L", PLOS, 8, pp.e53541~ (2013)