저온에서도 결구가 잘 형성되는 배가 반수체 배추 ‘원교20051호’ 개발
Developing Double-Haploid Inbred Lines of ‘Wonkyo20051ho’ Kimchi Cabbage (Brassica rapa. L) Characterized by Formation of Tight Head at Low Temperatures
5
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Kimchi cabbage (Chinese cabbage) is a very common Korean traditional vegetable, cultivated across 32,000 ha, which accounts for about 13% of the total vegetable cultivation area in Korea. Since 2010, frequent occurrences of extreme weather conditions caused a shortage of summer, winter, and autumn Kimchi cabbage. Therefore, the National Institute of Horticultural and Herbal Science (NIHHS) selected a haploid breeding method of microspore culture to develop inbred lines that show resistance under extremely low or high temperature for Kimchi cabbage production. ‘Wonkyo20051ho’ is a double-haploid (DH) inbred line developed using the microspore culture method. Eleven heat resistant parental plants were selected through summer cultivation of 37 resources collected in 2014. Microspore culture was efficient in regenerating four resources, from which 13 DH inbred lines were developed. After artificially inoculating ‘Seosan’ clubroot with low concentration inoculum, 7 inbred lines showing moderate resistance were selected. As a result of the autumn cultivation of 83 breeding plants, including 6 selected inbred lines, the ‘18-FH98’ inbred line forming tight heads with yellow inner leaf under low temperature was finally selected during the 2018 autumn field trial. The selected DH inbred line was named ‘Wonkyo20051ho’ and is expected to be a valuable breeding material possessing tolerance to low temperature and clubroot.
Abstract
Kimchi cabbage (Chinese cabbage) is a very common Korean traditional vegetable, cultivated across 32,000 ha, which accounts for about 13% of the total vegetable cultivation area in Korea. Since 2010, frequent occurrences of extreme weather conditions caused a shortage of summer, winter, and autumn Kimchi cabbage. Therefore, the National Institute of Horticultural and Herbal Science (NIHHS) selected a haploid breeding method of microspore culture to develop inbred lines that show resistance under extremely low or high temperature for Kimchi cabbage production. ‘Wonkyo20051ho’ is a double-haploid (DH) inbred line developed using the microspore culture method. Eleven heat resistant parental plants were selected through summer cultivation of 37 resources collected in 2014. Microspore culture was efficient in regenerating four resources, from which 13 DH inbred lines were developed. After artificially inoculating ‘Seosan’ clubroot with low concentration inoculum, 7 inbred lines showing moderate resistance were selected. As a result of the autumn cultivation of 83 breeding plants, including 6 selected inbred lines, the ‘18-FH98’ inbred line forming tight heads with yellow inner leaf under low temperature was finally selected during the 2018 autumn field trial. The selected DH inbred line was named ‘Wonkyo20051ho’ and is expected to be a valuable breeding material possessing tolerance to low temperature and clubroot.
참고문헌 (24)
- Braselton JP, "Current status of the plasmodiophorids", Crit Rev Microbiol, 21, pp.263~275 (1995)
- Cao MQ, "Embryogenesis and plant regeneration of pakchoi(Brassica rapa L. ssp. chinensis)via in vitro isolated microspore culture", Plant Cell Rep, 13, pp.447~450 (1994)
- Castlebury LA, "Small subunit ribosomal RNA gene phylogeny of Plasmodiophora brassicae", Mycologia, 90, pp.102~107 (1998)
- Cheah LH, "Biological control of clubroot on cauliflower with Trichoderma and Streptomyces", Spp Organics and Biocontrol, 53, pp.18~21 (2000)
- Chuong PV, "High frequency embryogenesis through isolated microspore culture in Brassica napus L. and Brassica Carinata braun L", Plant Sci, 39, pp.219~226 (1985)
- Datnoff LE, "Efficacy of chlorine for decontaminatining water infected with resting spores of Plasmodiophora brassicae", Plant Disease, 71, pp.734~736 (1987)
- 장하영, "소포자 배양으로 육성된 구가 크고 곧은 배추 계통 ‘원교20038호’", 한국육종학회지, 49(3), pp.256~264 (2017), 한국육종학회[DOI]
- 장하영, "잎이 얇고 작은 구를 형성하는 배추 계통 ‘원교20042호’ 육성", 한국육종학회지, 48(2), pp.184~191 (2016), 한국육종학회[DOI]
- Kawamura K, "Genetic distance of inbred lines of Chinese cabbage and its relationship to heterosis", Plant Gene, 5, pp.1~7 (2016)
- 김헌, "Pathotype Classification of Plasmodiophora brassicae Isolates Using Clubroot- Resistant Cultivars of Chinese Cabbage", The Plant Pathology Journal, 32(5), pp.423~430 (2016), 한국식물병리학회
- "Variety registlation" (2018)[URL]
- Kuginuki Y, "Variation in virulence of Plasmodiophora brassicae in Japan tested with clubroot resistant cultivars of Chinese cabbage(Brassica rapa L. ssp. pekinensis)", Eur J Plant Pathol, 105, pp.327~332 (1999)
- Lee SS, "Microspore culture of broccoli", J Kor Soc Hort Sci, 36, pp.635~640 (1995)
- Lichter, R, "Induction of haploid plants from isolated pollen of Brassica napus", Z. Pflanzenphysiol, 105, pp.427~434 (1982)
- Ministry of Agriculture, Food and Rural Affairs, "The statistics of vegetable production", ~ (2018)
- Nitsch N, "The induction of flowering in vitro in stem segments of Plumbago indica L. I. & II", The production of vegetative buds Planta, 72, pp.355~384 (1967)
- 박수형, "교배육종 및 소포자 배양에 의한 뿌리혹병 race4 저항성 배추 계통 육성", Korean Journal of Agricultural Science, 38(4), pp.613~618 (2011), 농업과학연구소
- Sato, T., "Plant regeneration from isoland microspore cultures of Chinese cabbage(Brassica campestris ssp. pekinensis)", Plant Cell Rep, 8, pp.486~488 (1989)
- Takahata Y, "High frequency embryogenesis and plant regeneration in isolated microspore culture of Brassica oleracea L", Plant Sci, 74, pp.235~242 (1991)
- Takahata Y, "Microspore culture of radish(Raphanus sativus L. ) : influence of genotype and culture conditions on embryogenesis", Plant Cell Rep, 16, pp.163~166 (1996)
- "The recent history of vegetable seed industry in Korea", pp.257~ (2008), SNU press
- Van de Peer Y, "An updated and comprehensive rRNA phylogeny(crown)of eukaryotes based on rate-calibrated evolutionary distances", J Mol Evol, 51, pp.565~576 (2000)
- Werner S, "Genetic mapping of clubroot resistance genes in oilseed rape", Theor Appl Genet, 116, pp.363~372 (2008)
- 용영록, "유용길항균이 배추 뿌리혹병 방제에 미치는 영향", 원예과학기술지, 21(3), pp.194~198 (2003), 한국원예학회