Micro -computed tomography를 이용한 골다공증의새로운 형태학적 연구기법
Micro --CT Evaluation in Osteoporosis Model
1
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
골다공증모델동물의 뼈잔기둥의 조직변화 및 골다공증모델연구에서 생체 내 미세 단층 촬영시스템(SKYSCAN1076, Skyscan, Belgium)을 적용하기 적합한 실험조건을 알아보고자 본시험을 수행하였다.암컷 흰쥐에게 난소적출과 저칼슘식이요법 (calcium free diet)을 시행하여 골다공증을 유발하였다. 골다공증의 유발된 흰쥐를 관류고정한후 허리뼈를 적출하여 탈회과정을 거친후 조직표본을 제작하여 골다공증모델동물의 뼈잔기둥에 대한 조직학적변화를 관찰하였다. 또한 흰쥐의 넷째 허리뼈를 35 µm의 해상도로 미세단층촬영을 시행하였다.골다공증 유발후 12주후에 척추뼈를 적출한후, 조직소견은 척추뼈의 뼈잔기둥의 두께가 감소하였고 일부 뼈잔기둥에서는 단락이 관찰되었으며, 뼈잔기둥내 산성점액질이 감소된 양상이 관찰되었다. 생체 내 미세 단층 촬영시스템의 평균 촬영시간은 20분정도 소요되었으며, pentobarbital, chloral hydrate, ketamine/xylazine 등의 마취제를 사용한 결과 ketamine/xylazine을 사용시 촬영에 가장 적절한 호흡주기(1.3~1.6/sec)를 유지하였으며, 마취제의 농도는 ketamine은 체중 kg당 50 mg, xylazine은 체중 kg당 6mg이 적정한 농도였다.이상의 결과를 종합한바, 난소적출과 저칼슘식이요법은 뼈잔기둥의 두께감소 및 산성점액질의 감소를 초래하여 뼈를 약화시키는 것으로 사료되며, 또한 고화질 생체내 미세단층촬영시스템을 이용하여 골다공증모델동물연구하는데 있어, 마취시간, 마취제 및 호흡주기가 중요한 요인이 되리라 사료된다.
Abstract
Osteoporosis is a major health-care problem for aging communities. And postmenopausal osteoporosis is a common disorder characterized by an increase in bone resorption relative to bone formation, generally in conjunction with an increased rate of bone turnover.Twenty female rats were used as rat model of osteoporosis. They randomly were divided into two groups:a normal control group, and calcium-free diet groups treated with bilateral ovariectomy. The animals were sacrified at 12 weeks after treatment. In the rats with osteoporosis, there was significant thining and loss of trabeculae, accompanied by a disconnection of trabeculae. And acid mucopolysaccharides were decreased in osteoporotic animals. In order to setup conditions of invivo micro-CT, proper anesthetic is ketamine (50 mg/kg)/xylazine (6 mg/kg). And proper breathing rate of experimental animals is 1.3~1.6/sec. Our finding suggest that bilateral ovariectomy and calcium-free diet induce decrease in thickeness of trabecular bone and decrease in acid mucopolysaccharide, therefore cause weakness of bone. And micro-CT imaging system is useful to evaluate trabecular bone. Anesthetic time, anesthetics and respiration rate also signify much in micro-CT imaging system.
참고문헌 (17)
- "Skeletal effects of Zinc deficiency in growing rats. JTrace Elem Med Biol 13", pp.21~26 (1999)
- "Secondary osteoporosis. Aging Clin Exp Res", 10, pp.214~224 (1998)
- "Animal models of immobilization osteopenia.Morphologie 83", pp.25~34 (1999)
- "Morphologicalchanges of cochlear structures after cisplatin administration.Korean J Otolaryngol 45", pp.538~543 (2002)
- "Relationships between marrow fatand bone turnover in ovariectomized and intact rats. Bone.12", pp.123~131 (1991)
- "Biologic significance of hyaluronic acid and hyalunonidase.Physiol 27", pp.335~ (1949)
- "Relativeand combined effects of ethanol and protein deficiency onbone histology and mineral metabolism. Alcohol 20", pp.1~8 (2000)
- "Alcian blue techniques for histochemical studyof acidic carbohydrates. J Histochem Cytochem" (1956)
- "A review ofhigh-resolution X-ray computed tomography and otherimaging modalities for small animal research. Lab Anim", 30, pp.36~45 (2001)
- "The biochemical and histochemical nomenclature of mucins. British Medical Bulletin", 34, pp.1~8 (1978)
- "A microtomographic systemfor the nondestructive evaluation of bone architecture.Calcif Tissue Int 58", pp.24~29 (1996)
- "Magnesium deficiency-induced osteroporosisin the rat uncoupling of bone formation and bone resorption.Magnes Res 12", pp.257~267 (1999)
- "Effect of Cephalosporinson growth plates of femoral heads of Rats. J Kor OrthoAsso 34", pp.351~356 (1999)
- "Effects of cyclosporine A on sex hormone and estrogenreceptor in male rat with special reference to cyclosporin Ainducedosteoporosis. Yonsei Med J 41", 41, pp.61~67 (2000)
- "A new stain for mucin.Quart J Microbiol Sci 91", pp.477~479 (1959)
- "Effects of lifelong nicotine inhalation onbone mass and mechanical properties in female rat femurs.Calcif Tissue Int 65", (rdsletten l), pp.246~249 (1999)
- "Uncouplingbetween bone formation and resorption and resorption inovariectomized with chronic cadmium exposure. Toxicol.Appl Pharmacol 164", pp.264~272 (2000)