淸肌祛痒湯의 RBL-2H3 비만세포와 OVA/alum 감작 생쥐의 알레르기 염증 반응 억제 효과
Suppressive Effect of CheongGiGeoYangTang on Allergic Inflammation of RBL-2H3 Mast Cells and OVA/alum-sensitized Mice
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Objectives : 청기거양탕은 임상에서 항알레르기 목적으로 다양하게 사용되고 있지만 그 효과에 대한 실험적 연구는 아직 보고되어 있지 않다. 따라서 본 연구는 청기거양탕의 항알레르기 효과를 규명하기 위해 이루어졌다. Methods : 본 연구에서 청기거양탕이 RBL-2H3 비만세포의 IL-4와 IL-13을 조절할 수 있는지 Real-Time PCR과 ELISA로 분석하였고, GATA-1, GATA-2, NFAT-1, NFAT-2, c-Jun, c-Fos, NF-κB p65 전사인자 발현 억제를 western blot으로 관찰했다. 또한 오발부민 알레르기 모델을 통해 OVA-specific IgE와 Th2 cytokine IL-4, IL-13의 생성을 관찰하였다. Results : 청기거양탕을 처리한 RBL-2H3 비만세포에서 IL-4와 IL-13 mRNA 유전자 발현과 단백질 생성은 현저하게 억제 되었으며, western blot을 통해 관찰된 전사인자 발현은 GATA-1, GATA-2, NFAT-1, NFAT-2, c-Fos, NF-κB p65의 발현은 억제되었으나 c-Jun은 억제 되지 않았다. 청기거양탕을 투여한 OVA/alum 감작 생쥐의 OVA-specific IgE와 IL-4, IL-13 생성도 억제 되었다. Conclusions : 청기거양탕이 활성화된 비만세포와 Th2세포에서 발현하는 IL-4, IL-13의 생성을 억제하여 알레르기 염증 반응을 조절하는 것으로 사료된다.
Abstract
Objectives CheongGiGeoYangTang has been used for anti-allergenic purpose. However there was no experimental study about its effect. Therefore, this study was designed to investigate the anti-allergenic effect of CheongGiGeoYangTang. Methods Modifiability of RBL-2H3 mast cells' IL-4, IL-13 was analyzed by qRT-PCR and ELISA. Also, the suppressive effect of GATA-1, GATA-2, NF-AT1, NF-AT2, AP-1 and NF-κB p65 transcription factors was observed by western blotting. OVA-specific IgE, IL-4 and IL-13 production in ovalbumin allergy model was examined as well. Results It was showed that the RBL-2H3 mast cells treated with CheongGiGeoYangTang extract(CGGYT) was significantly suppressed mRNA expression, production of IL-4 and IL-13, and prominently inhibited the expression of transcription factors including GATA-1, GATA-2, NFAT-1, NFAT-2, c-Fos and NF-κB p65 but not c-Jun. The administration of CGGYT was suppressed the amount of OVA-specific IgE, IL-4 and IL-13 in OVA/alum-sensitized mice. Conclusions We considered CGGYT would regulate the allergic inflammation as inhibition of IL-4 and IL-13 production in activated mast cells and Th2 cells.
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