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蒲公英이 MC/9 mast cell에서의 Th2 cytokine 발현에 미치는 영향

Effects Of TAM (Taraxacum mongolicum) On Th2 Cytokine Production In MC/9 Mast Cells

안택원|최재송|배나영|장문희
사상체질면역의학회 (The Society of Sasang Constitution and Immune Medicine)|2012.03|사상체질면역의학회지 (Journal of Sasang Constitution and Immune Medicine)|제24권 제1호|pp. 54-65

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참고문헌

키워드

CytokineTAM (Taraxacum mongolicum)MC/9 Mast CellAtopic Dermatitis.
주제분류한의학

초록

1. Objectives The purpose of this study is to investigate the effects of TAM (Taraxacum mongolicum) on Th2 cytokine production in MC/9 mast cells. 2. Methods The effects of TAM was analyzed by ELISA and Real-time PCR in MC/9 mast cells. Levels of IL-5, IL-13 were measured using enzyme-linked immunosorbent assays(ELISA). mRNA levels of IL-4, IL-5, IL-6, IL-13 were analyzed with Real-time PCR. 3. Results 1) TAM inhibited the IL-4 production significantly in comparison to PI-control group at concentration of 50 ㎍/㎖, 100 ㎍/㎖, 200 ㎍/㎖. 2) TAM inhibited the IL-13 production significantly in comparison to PI-control group at concentration of 50 ㎍/㎖, 100 ㎍/㎖, 200 ㎍/㎖. 3) TAM inhibited the IL-4 mRNA expression significantly in comparison to PI-control group at concentration of 100 ㎍/㎖. 4) TAM inhibited the IL-5 mRNA expression significantly in comparison to PI-control group at concentration of 50 ㎍/㎖, 100 ㎍/㎖. 5) TAM inhibited the IL-6 mRNA expression significantly in comparison to PI-control group at concentration of 100 ㎍/㎖. 6) TAM inhibited the IL-13 mRNA expression significantly in comparison to PI-control group at concentration of 100 ㎍/㎖. 4. Conclusion These results indicate that TAM (Taraxacum mongolicum) has the effect of decreasing the Th2 cytokine production in the MC/9 mast cell.

Abstract

1. Objectives The purpose of this study is to investigate the effects of TAM (Taraxacum mongolicum) on Th2 cytokine production in MC/9 mast cells. 2. Methods The effects of TAM was analyzed by ELISA and Real-time PCR in MC/9 mast cells. Levels of IL-5, IL-13 were measured using enzyme-linked immunosorbent assays(ELISA). mRNA levels of IL-4, IL-5, IL-6, IL-13 were analyzed with Real-time PCR. 3. Results 1) TAM inhibited the IL-4 production significantly in comparison to PI-control group at concentration of 50 ㎍/㎖, 100 ㎍/㎖, 200 ㎍/㎖. 2) TAM inhibited the IL-13 production significantly in comparison to PI-control group at concentration of 50 ㎍/㎖, 100 ㎍/㎖, 200 ㎍/㎖. 3) TAM inhibited the IL-4 mRNA expression significantly in comparison to PI-control group at concentration of 100 ㎍/㎖. 4) TAM inhibited the IL-5 mRNA expression significantly in comparison to PI-control group at concentration of 50 ㎍/㎖, 100 ㎍/㎖. 5) TAM inhibited the IL-6 mRNA expression significantly in comparison to PI-control group at concentration of 100 ㎍/㎖. 6) TAM inhibited the IL-13 mRNA expression significantly in comparison to PI-control group at concentration of 100 ㎍/㎖. 4. Conclusion These results indicate that TAM (Taraxacum mongolicum) has the effect of decreasing the Th2 cytokine production in the MC/9 mast cell.

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