뼈모세포 초기부착에 영향을 미치는 나노수준 거칠기 칼슐포스페이트 박막 개발#

Abstract

Surface roughness is well known to affect cellular activity. This study presents a method to fabricate nano-scale surface roughness on the surfaces of polymer, metal, glass with calcium phosphate thin film. Metastable calcium and phosphate ion solution was slowly heated to obtain calcium phosphate thin fibn. Then fresh calcium and phosphate ion solution was added to fabricate nano-scale roughness over tlùs initially forrned thin film. Various surfaces of nano- scale roughness lower than 800 nm of Ra v외ue was obtained. Initial cell adhesion was characterized over the fabricated surfaces using MC3T3-El osteoblastic cellline. Spreading of osteoblastic cell decreased according to the increase of surface roughness. Less stress fibers developed in the cells attaching to the rougher surface. Less focal contacts visualized by staining vinculin was found in the cells of irútial adhesion. All these features of the initial cell adhesion represents that less adhesion signals are generated from the rougher surface. Further study is required to evaluate the effect of these low adhesion signals on the overall cellular activitv.