Effect of titanium surface roughness on cell adhesion of human osteoblast-like cells [MG63]
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Abstract
Statement of problem. The effects of surface roughness have not or insufficiently been analyzed on earlier events such as cell adhesion though cell behavior most germane to implant performance is cell adhesion. Purpose. The purpose of this study was to evaluate cell adhesion of osteoblast-like cells (MG63) onto three types of titanium disks with varying roughness using the Elisa assay. Materials and methods. Representative disks from each group (SLA, HA, machined) were subjected to surface analysis and surface roughness was measured by the optical interferometer (Accura 2000, Intekplus Co., Seoul, Korea). Following this, MG63 cells were cultured on the titanium disks and released. Cell adhesion measurements using the Elisa assay were performed specifically at three points: after 24, 48, and 72 hours of culture. Results. Among the 3 types of surface analyzed, the SLA surface was the roughest with a Ra value of 1.114 μm followed by HA coated surface and machined surface, consecutively. The optical density values for the SLA surface group was significantly higher than that of the machined and HA coated suface groups following 24 and 48 hours of culture. The cell culture on HA coated surface showed significantly higher values compared to the machined surface following 24, 48 and 72 hours of culture. Conclusion. The results suggest that surface treatment of titanium surfaces enhanced cell adhesion of human osteoblast-like cells (MG63).
참고문헌 (19)
- "Osseointegration and its experi-mental background", 50, pp.399~410
- "Introduction to osseointegration", pp.11~76 (1985), Branemark P-I in Branemark P-I Osseointegrationin Clinical Dentistry QuintessencePublishing Co
- "Biomaterial and implant sur-faces:A surface science approach", 3, pp.247~259 (1988)
- "Osseointegration andmatrix production at the implant surface", 2, pp.83~101 (1991)
- "The influence of sputtered bonesubstitutes on cell growth and phenotypic ex-265pression", pp.205~213, Toronto Press 1991
- "Effect of titanium surface roughness onproliferation and protein synthe-sis of human osteoblast-like cells (MG63).", 29, pp.389~401 (1995)
- "Titanium surface roughness alters responsive-ness of MG63 osteoblast-like cells to 1α,25-(OH)2D3", 39, pp.77~85 (1998)
- "Optimization of surface micro-morphology for enhanced osteoblast responsesin vitro", 7, pp.302~310 (1992)
- "In vitro connective tissue cell at-tachment to cpTi", 68, pp.276~
- "Effect of titanium surfacecharacteristics on chondrocytes and osteoblasts invitro", 5, pp.323~335 (1995)
- "The effects of implant surface topog-raphy on the behavior of cells", 3, pp.231~246 (1988)
- "Influence of surface characteristics onbone integration of titanium implants.A histo-morphometric study in miniature pigs", 25, pp.889~902 (1991)
- "In vitro modeling of the bone", implantinterface, 1996, pp.245, 426~445
- "The bone-titaniuminterface in vitro", 24, pp.1289~ (1990)
- "Hjorting-Hansen E. Anchorage ofTiO2-blasted, HA-coated, and machined implants:An experimental study with rabbits.", 29, pp.1223~1231 (1995)
- "Surfaceroughness modulates the local production ofgrowth factors and cytokines by osteoblast-likeMG63 cells", 32, pp.55~63 (1996)
- "Bone response to surfacemodified titanium implants:studies on electrop-olished implants with different oxide thicknessesand morphology", 15, pp.1062~1074 (1994)
- "Bone responseto surface-modified titanium implants:studieson the early tissue response to machines and elec-tropolished implants with different oxide thicknesses", 17, pp.605~616 (1996)
- "Direct bone formation on sand-blasted titanium implants:an experimental study", 17, pp.1015~1018 (1996)