The effect of magnetic fields on the MC3T3-E1 cells attachment
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
To determine the effects of magnetic fields by comparing interaction between the osteoblast and titanium dental implant with a magnet which is inserted in the specially designed implant abutment. Dental implants connected with a magnet - inserted abutment were used. A cell-spreading assay in which percentages of cells at four different stages of attachment were identified by scanning electron microscopy and quantified within 30 min. 4hour and 24hour attachment periods. The surfaces of dental implant connected with a MagScrew showed significantly enhanced rates of cell spreading in comparison with the others. Based on these observations. it appears reasonable to suggest that magnetic fields promote the differentiation and maturation of osteoblasts by affecting the expression of adhesion proteins imbedded in the cell membrane and may have a favorable effect on immediateloading implant treatment by reducing the processing time for mineralization.
Abstract
To determine the effects of magnetic fields by comparing interaction between the osteoblast and titanium dental implant with a magnet which is inserted in the specially designed implant abutment. Dental implants connected with a magnet - inserted abutment were used. A cell-spreading assay in which percentages of cells at four different stages of attachment were identified by scanning electron microscopy and quantified within 30 min. 4hour and 24hour attachment periods. The surfaces of dental implant connected with a MagScrew showed significantly enhanced rates of cell spreading in comparison with the others. Based on these observations. it appears reasonable to suggest that magnetic fields promote the differentiation and maturation of osteoblasts by affecting the expression of adhesion proteins imbedded in the cell membrane and may have a favorable effect on immediateloading implant treatment by reducing the processing time for mineralization.
참고문헌 (48)
- Schwartz. Z., "Underlying mechanisms at the bone-biomateria1 interface", Journal of Cellular Biochemistry, 56, pp.340~347 (1994)
- Kieswetter. K., "The role of implant surface characteristics in the healing of bone", Critical Reviews in Oral Biology and Medicine, 7, pp.329~345 (1996)
- Sammons RL, "Comparison of osteoblast spreading on microstructured dental implant surfaces and cell behaviour in an explant model of osseointegration. A scanning electron microscopic study", Clin Oral Implants Res, 16(6), pp.657~666 (2005)
- Langer R, "Tissue engineering", Science, 260(5110), pp.920~926 (1993)
- Fleming JE. Jr., "Bone cells and matrices in orthopedic tissue engineering", Orthop Clin North Am, 31(3), pp.357~374 (2000)
- Burg KJ, "Biomaterial developments for bone tissue engineering", Biomaterials, 21(23), pp.2347~2359 (2000)
- Hutmacher DW, "Scaffolds in tissue engineering bone and cartilage", Biomaterials, 21(24), pp.2529~2543 (2000)
- Botchwey EA, "Bone tissue engineering in a rotating bioreactor using a microcarrier matrix system", J Biomed Mater Res, 55(2), pp.242~253 (2001)
- Yamaguchi A, "Effects of BMP-2 BMP-4. and BMP-6 on oste oblastic differentiation of bone marrowderived stromal cell lines. 8T2 and MC3T3-G2", PA6, 1996, pp.220(2), 366~371
- Lu L, "TGF-beta1 release from biodegradable polymer microparticles: Its effects on marrow stromal osteoblast function", J Bone Joint Surg Am, 83-A(Suppl 1. Pt 2), pp.882~891 (2001)
- Steinbrech DS, "VEGF expression in an osteoblast-like cell line is regulated by a hypoxia response mechanism", Am J Physiol Cell Physiol, 278(4), pp.C853~C860 (2000)
- Kasukawa Y, "lnsulin-like growth factor I effect on the number of osteoblast progenitors is impaired in ovariectomized mice", J Bone Miner Res, 17(9), pp.1579~1587 (2002)
- Boden SD, "Bioactive factors for bone tissue enginee ring", Clin Orthop Relat Res, 367(Suppl), pp.884~894 (1999)
- Rubin CT, "Prevention of osteoporosis by pulsed electromagnetic fields", J Bone Joint Surg Am, 71(3), pp.411~417 (1989)
- Tabrah F, "Bone density changes in osteoporosis-prone women exposed to pulsed electromagnetic fields (PEMFs)", J Bone Miner Res, 5(5), pp.437~442 (1990)
- Chang K, "Pulsed electromagnetic fields stimulation affects osteoclast formation by modulation of osteoprotegerin. RANK ligand and macrophage colony-stimulating factor", J Orthop Res, 23(6), pp.1308~1314 (2005)
- De Mattei M, "Correlation between pulsed electromagnetic fields exposure time and cell proliferation increase in human osteosarcoma cell lines and human normal osteoblast cells in vitro", Bioelectromagnetics, 20(3), pp.177~182 (1999)
- Diniz P, "Effects of pulsed electromagnetic field (PEMF) stimulation on bone tissue like formation are dependent on the maturation stages of the osteoblasts", Bioelectromagnetics, 23(5), pp.398~405 (2002)
- Chang WH, "Effect of pulseburst electromagnetic field stimulation on osteoblast cell acti vi ties", Bioelectromagnetics, 25(6), pp.457~465 (2004)
- Tsai MT, "Pulsed electromagnetic fields affect osteoblast proliferation and differentiation in bone tissue engineering", Bioelectromagnetics, 28(7), pp.519~528 (2007)
- Rajaraman. R., "A scanning electron microscope study of cell adhesion and spreading in vitro", Experimental Cell Research, 88, pp.327~339 (1974)
- Darendeliler. M. A., "The effects of samarium-cobalt magnets and pulsed electromagnetic fields on tooth movement", Am. J. Orthod. Dentofac. Orthop, 107, pp.578~588 (1995)
- Parkinson. W. C., "Comments on the use of electromagnetic fields in biological studies", Calicif. Tissue Int, 37, pp.198~207 (1985)
- Yan. Q. C., "Effects of static magnetic field on bone formation of rat femurs", Med. Eng. Phys, 20, pp.397~402 (1998)
- Shimizu. E., "Static magnetic fields induced bone sialoprotein (BSP) expression is mediated through FGF2 response element and pituitary-specific transcription factor-1 motif", J. Cell Biochem, 91, pp.1183~1196 (2004)
- Yamamoto. Y., "Effects of static magnetic fields on bone formation in rat osteoblast cultures", J. Dent. Res, 82, pp.962~966 (2003)
- Diniz. P., "Effects of pulsed electromagnetic field (PEMF) stimulation on bone tissue like formation are dependent on the maturation stages of the osteoblasts", Bioelectromagnetics, 23, pp.398~405 (2002)
- Lohmann Schwartz. C. H. Z., "Pulsed electromagnetic field stimulation of MG 63 osteoblast-like cells affects differentiation and local production", J. Orthop. Res, 18, pp.637~646 (2000)
- Stanford, C.M., "Bone cell expression on titanium surfaces is altered by sterilization treatments", Journal of Dental Research, 73, pp.1061~1071 (1994)
- Vezeau. P.J., "Effects of multiple sterilization on surface characte ristics and in vitro biologic responses to titanium", Journal of Oral and axillofacial Surgery, 54, pp.738~746 (1996)
- Bowers KT, "Optimization of surface micromorphology for enhanced osteoblast responses in vitro", Int J Oral Maxillofac Implants, 7, pp.302~310 (1992)
- Keller JC, "Characterizations of titanium implant surfaces", J Biomed Mater Res, 28, pp.939~946 (1994)
- Swart KM, "Short-term plasma-cleaning treatments enhance in vitro osteoblast attachment to titanium", J Oral Implantol, 18, pp.130~137 (1992)
- Vezeau PJ, "Effects of multiple s t erili zation on surface characteristics and in vitro biologic responses to titanium", J Oral Maxillofac Surg, 54, pp.738~746 (1996)
- Stanford CM, "Bone cell expression on titanium surfaces is altered by sterilization treatments", J Dent Res, 73, pp.1061~1071 (1994)
- Chang Y-L, "Osteoblastic cell attachment to hydroxyapatite-coated implant surfaces in vitro", Int J Oral Maxillofac Implants, 14, pp.239~247 (1999)
- Brunette DM, "The effects of implant surface topography on the behavior of cells", Int J Oral Maxillofac Implants, 3, pp.231~246 (1988)
- Malik MA, "Osteoblasts on hydroxyapatite, alumina and bone surfaces in vitro Morphology during the first 2h of attachment", Biomaterials, 13, pp.123~128 (1992)
- Schakenraad JM, "Kinetics of cell spreading on protein pre-coated substrata: A study of interfaci al aspects", Biomat erials, 10, pp.43~50 (1989)
- Howlett CR, "Mechanism of initial attachment of cells deri ved from human bone to commonly used prosthetic materia1s during cell-culture", Biomateria1s, 15, pp.213~222 (1994)
- Zreiqat H, "The ro1e of surface chara cteristics in the ini tial adhesion of human bone-derived cells on ceramics", Cells Mater, 6, pp.45~56 (1996)
- Aoki. H., "Effects of static magnetic fields on membrane permeability of a cultured cell line", Res. Comm. Chem. Pathol. Pharm, 69, pp.103~106 (1990)
- Camilleri. S., "Static magnetic field effects on the sagittal suture in Rattus Norvegicus", Am. J. Orthod. Dentofac. Orthop, 103, pp.240~246 (1993)
- Kotani. H., "Strong static magnetic field stimulates bone formation to a definite orientation in vitro and in vivo", J. Bone Miner. Res, 17, pp.1814~1821 (2002)
- Suda. T., "Magnetic orientation of red blood cell membranes", I.E.E.E. Trans. Magn, 30, pp.4713~4715 (1994)
- Suda. T., "Effect of strong magnetic fields on the characteristics of bilayer lipid membranes", J. Appl. Phys, 81, pp.4318~4320 (1997)
- Coots. A., "Effect of a 0.5-T static magnetic fie1d on conduction in guinea pig spinal cord", J Neurol. Sci, 222, pp.55~57 (2004)
- Silva. V. S., "Aluminum accumulation and membrane fluidity alteration in synaptosomes isolated from rat brain", Neurosci. Res, 44, pp.181~193 (2002)
