Static magnetic fields promote osteoblastic/cementoblastic differentiation in osteoblasts, cementoblasts, and periodontal ligament cells
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Purpose: Although static magnetic fields (SMFs) have been used in dental prostheses and osseointegrated implants, their biological effects on osteoblastic and cementoblastic differentiation in cells involved in periodontal regeneration remain unknown. This study was undertaken to investigate the effects of SMFs (15 mT) on the osteoblastic and cementoblastic differentiation of human osteoblasts, periodontal ligament cells (PDLCs), and cementoblasts, and to explore the possible mechanisms underlying these effects. Methods: Differentiation was evaluated by measuring alkaline phosphatase (ALP) activity, mineralized nodule formation based on Alizarin red staining, calcium content, and the expression of marker mRNAs assessed by reverse transcription polymerase chain reaction (RT-PCR). Signaling pathways were analyzed by western blotting and immunocytochemistry. Results: The activities of the early marker ALP and the late markers matrix mineralization and calcium content, as well as osteoblast- and cementoblast-specific gene expression in osteoblasts, PDLCs, and cementoblasts were enhanced. SMFs upregulated the expression of Wnt proteins, and increased the phosphorylation of glycogen synthase kinase-3β (GSK-3β) and total β-catenin protein expression. Furthermore, p38 and c-Jun N-terminal kinase (JNK) mitogen-activated protein kinase (MAPK), and nuclear factor-κB (NF-κB) pathways were activated. Conclusions: SMF treatment enhanced osteoblastic and/or cementoblastic differentiation in osteoblasts, cementoblasts, and PDLCs. These findings provide a molecular basis for the beneficial osteogenic and/or cementogenic effect of SMFs, which could have potential in stimulating bone or cementum formation during bone regeneration and in patients with periodontal disease.
Abstract
Purpose: Although static magnetic fields (SMFs) have been used in dental prostheses and osseointegrated implants, their biological effects on osteoblastic and cementoblastic differentiation in cells involved in periodontal regeneration remain unknown. This study was undertaken to investigate the effects of SMFs (15 mT) on the osteoblastic and cementoblastic differentiation of human osteoblasts, periodontal ligament cells (PDLCs), and cementoblasts, and to explore the possible mechanisms underlying these effects. Methods: Differentiation was evaluated by measuring alkaline phosphatase (ALP) activity, mineralized nodule formation based on Alizarin red staining, calcium content, and the expression of marker mRNAs assessed by reverse transcription polymerase chain reaction (RT-PCR). Signaling pathways were analyzed by western blotting and immunocytochemistry. Results: The activities of the early marker ALP and the late markers matrix mineralization and calcium content, as well as osteoblast- and cementoblast-specific gene expression in osteoblasts, PDLCs, and cementoblasts were enhanced. SMFs upregulated the expression of Wnt proteins, and increased the phosphorylation of glycogen synthase kinase-3β (GSK-3β) and total β-catenin protein expression. Furthermore, p38 and c-Jun N-terminal kinase (JNK) mitogen-activated protein kinase (MAPK), and nuclear factor-κB (NF-κB) pathways were activated. Conclusions: SMF treatment enhanced osteoblastic and/or cementoblastic differentiation in osteoblasts, cementoblasts, and PDLCs. These findings provide a molecular basis for the beneficial osteogenic and/or cementogenic effect of SMFs, which could have potential in stimulating bone or cementum formation during bone regeneration and in patients with periodontal disease.
참고문헌 (40)
- Shimono M, "Regulatory mechanisms of periodontal regeneration", Microsc Res Tech, 60, pp.491~502 (2003)
- Cook JJ, "Healing in the new millennium : bone stimulators : an overview of where we've been and where we may be heading", Clin Podiatr Med Surg, 32, pp.45~59 (2015)
- Funk RH, "Electromagnetic effects-from cell biology to medicine", Prog Histochem Cytochem, 43, pp.177~264 (2009)
- Gaetani R, "Differentiation of human adult cardiac stem cells exposed to extremely low-frequency electromagnetic fields", Cardiovasc Res, 82, pp.411~420 (2009)
- Sakata M, "The effects of a static magnetic field on orthodontic tooth movement", J Orthod, 35, pp.249~254 (2008)
- Zhang J, "The effects of static magnetic fields on bone", Prog Biophys Mol Biol, 114, pp.146~152 (2014)
- Markov MS, "Magnetic field therapy : a review", Electromagn Biol Med, 26, pp.1~23 (2007)
- Yang TC, "Magnetic attachment for implant overdentures : influence of contact relationship with the denture base on stability and bending strain", Int J Prosthodont, 26, pp.563~565 (2013)
- Aksu AE, "Intraoral use of extraoral implants for oral rehabilitation of a pediatric patient after resection of ewing sarcoma of the mandible and reconstruction with iliac osteocutaneous free flap", J Craniofac Surg, 25, pp.930~933 (2014)
- Siadat H, "Effect of static magnetic fields on the osseointegration of immediately placed implants : a randomized controlled clinical trial", Implant Dent, 21, pp.491~495 (2012)
- Leesungbok R, "The effects of a static magnetic field on bone formation around a sandblasted, large-grit, acid-etched–treated titanium implant", J Oral Implantol, 39, pp.248~255 (2013)
- Yamamoto Y, "Effects of static magnetic fields on bone formation in rat osteoblast cultures", J Dent Res, 82, pp.962~966 (2003)
- Denaro V, "Periprosthetic electrochemical corrosion of titanium and titanium-based alloys as a cause of spinal fusion failure", Spine, 33, pp.8~13 (2008)
- Denaro V, "Static electromagnetic fields generated by corrosion currents inhibit human osteoblast differentiation", Spine, 33, pp.955~959 (2008)
- Chiu KH, "Static magnetic fields promote osteoblastlike cells differentiation via increasing the membrane rigidity", Ann Biomed Eng, 35, pp.1932~1939 (2007)
- Hsu SH, "The static magnetic field accelerates the osteogenic differentiation and mineralization of dental pulp cells", Cytotechnology, 62, pp.143~155 (2010)
- Kim EC, "Effects of moderate intensity static magnetic fields on human bone marrow-derived mesenchymal stem cells", Bioelectromagnetics, 36, pp.267~276 (2015)
- Bartold PM, "Molecular and cell biology of healthy and diseased periodontal tissues", Periodontol 2000, 40, pp.29~49 (2006)
- Miyakoshi J, "Effects of static magnetic fields at the cellular level", Prog Biophys Mol Biol, 87, pp.213~223 (2005)
- Wang Y, "A theoretical study of bone remodelling under PEMF at cellular level", Comput Methods Biomech Biomed Engin, 15, pp.885~897 (2012)
- Chung JH, "Deferoxamine promotes osteoblastic differentiation in human periodontal ligament cells via the nuclear factor erythroid 2-related factormediated antioxidant signaling pathway", J Periodontal Res, 49, pp.563~573 (2014)
- Kitagawa M, "Characterization of established cementoblast-like cell lines from human cementum-lining cells in vitro and in vivo", Bone, 39, pp.1035~1042 (2006)
- Pi SH, "Differential expression of periodontal ligamentspecific markers and osteogenic differentiation in human papilloma virus 16-immortalized human gingival fibroblasts and periodontal ligament cells", J Periodontal Res, 42, pp.104~113 (2007)
- Beertsen W, "The periodontal ligament: a unique, multifunctional connective tissue", Periodontol 2000, 13, pp.20~40 (1997)
- Chen FM, "Periodontal tissue engineering and regeneration : current approaches and expanding opportunities", Tissue Eng Part B Rev, 16, pp.219~255 (2010)
- Kim MB, "Kirenol stimulates osteoblast differentiation through activation of the BMP and Wnt", β-catenin signaling pathways in MC3T3-E1 cells, 2014, pp.98, 59~65
- Nöth U, "Activation of p38 and Smads mediates BMP-2 effects on human trabecular bone-derived osteoblasts", Exp Cell Res, 291, pp.201~211 (2003)
- Franceschi RT, "Regulation of the osteoblast-specific transcription factor, Runx2: responsiveness to multiple signal transduction pathways", J Cell Biochem, 88, pp.446~454 (2003)
- Subramaniam M, "Further characterization of human fetal osteoblastic hFOB 1.19 and hFOB", ER alpha cells: bone formation in vivo and karyotype analysis using multicolor fluorescent in situ hybridization, 2002, pp.87, 9~15
- Hayami T, "Dexamethasone's enhancement of osteoblastic markers in human periodontal ligament cells is associated with inhibition of collagenase expression", Bone, 40, pp.93~104 (2007)
- Bodine PV, "Wnt signaling and osteoblastogenesis", Rev Endocr Metab Disord, 7, pp.33~39 (2006)
- Reya T, "Wnt signalling in stem cells and cancer", Nature, 434, pp.843~850 (2005)
- Zhou J, "Effects of pulsed electromagnetic fields on bone mass and Wnt", β-catenin signaling pathway in ovariectomized rats, 2012, pp.43, 274~282
- Du L, "Extremely low frequency magnetic fields inhibit adipogenesis of human mesenchymal stem cells", Bioelectromagnetics, 35, pp.519~530 (2014)
- Wolf-Goldberg T, "Low electric fields induce ligand-independent activation of EGF receptor and ERK via electrochemical elevation of H(+)and ROS concentrations", Biochim Biophys Acta, 1833, pp.1396~1408 (2013)
- Ma J, "Magnetic stimulation modulates structural synaptic plasticity and regulates BDNF-TrkB signal pathway in cultured hippocampal neurons", Neurochem Int, 62, pp.84~91 (2013)
- Sun W, "Exposure to power-frequency magnetic fields can induce activation of P38 mitogen-activated protein kinase", Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi, 20, pp.252~255 (2002)
- Soda A, "Effect of exposure to an extremely low frequencyelectromagnetic field on the cellular collagen with respect to signaling pathways in osteoblast-like cells", J Med Invest, 55, pp.267~278 (2008)
- Vincenzi F, "Pulsed electromagnetic fields increased the anti-inflammatory effect of A2A and A3 adenosine receptors in human T", C-28a2 chondrocytes and hFOB 1.19 osteoblasts, 2013, pp.8, e65561~
- Sakurai T, "Enhanced secretion of prostaglandin E2 from osteoblasts by exposure to a strong static magnetic field", Bioelectromagnetics, 29, pp.277~283 (2008)