Evaluation of the Ameliorating Effects of oraCMU on Peri-Implantitis in an In Vitro Model
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Peri-implantitis, a major threat to the long-term success of dental implants, is initiated by periodontopathic bacteria, leading to excessive host inflammatory responses and increased matrix metalloproteinases (MMPs), which result in the loss of surrounding supporting tissues. This study aimed to investigate the anti-inflammatory and tissue-protective effects of the oral probiotic oraCMU in an in vitro human gingival fibroblast (HGFs) culture model on titanium discs that mimics the key pathogenesis of peri-implantitis. HGFs were cultured on sterilized titanium discs to simulate the peri-implant environment. Inflammation was induced by treatment with heat-killed Porphyromonas gingivalis (P. gingivalis). The efficacy of oraCMU was analyzed by pre-treating the cells before the P. gingivalis challenge. Real-time PCR was used to quantify the mRNA expression of the pro-inflammatory cytokines TNF-α, IL-6, IL-1β and MMP-1 and MMP-3. P. gingivalis treatment significantly increased the mRNA expression of major pro-inflammatory cytokines TNF-α and IL-6 in HGFs compared to the control group. In the oraCMU pre-treated group, the expression of TNF-a and IL-6 was significantly suppressed compared to the P. gingivalis-only group. Importantly, oraCMU also significantly reduced the mRNA expression of the tissue-destructive mediators MMP-1 and MMP-3. The inhibitory effect on IL-1β expression was marginal. In conclusion, the oral probiotic oraCMU demonstrated anti-inflammatory and tissue-protective effects in the peri-implantitis cell model by effectively suppressing the expression of inflammatory and tissue-destructive factors. These findings provide foundational scientific evidence that oraCMU can be utilized as a safe and effective probiotic-based strategy for the prevention and adjunctive treatment of peri-implantitis.
Abstract
Peri-implantitis, a major threat to the long-term success of dental implants, is initiated by periodontopathic bacteria, leading to excessive host inflammatory responses and increased matrix metalloproteinases (MMPs), which result in the loss of surrounding supporting tissues. This study aimed to investigate the anti-inflammatory and tissue-protective effects of the oral probiotic oraCMU in an in vitro human gingival fibroblast (HGFs) culture model on titanium discs that mimics the key pathogenesis of peri-implantitis. HGFs were cultured on sterilized titanium discs to simulate the peri-implant environment. Inflammation was induced by treatment with heat-killed Porphyromonas gingivalis (P. gingivalis). The efficacy of oraCMU was analyzed by pre-treating the cells before the P. gingivalis challenge. Real-time PCR was used to quantify the mRNA expression of the pro-inflammatory cytokines TNF-α, IL-6, IL-1β and MMP-1 and MMP-3. P. gingivalis treatment significantly increased the mRNA expression of major pro-inflammatory cytokines TNF-α and IL-6 in HGFs compared to the control group. In the oraCMU pre-treated group, the expression of TNF-a and IL-6 was significantly suppressed compared to the P. gingivalis-only group. Importantly, oraCMU also significantly reduced the mRNA expression of the tissue-destructive mediators MMP-1 and MMP-3. The inhibitory effect on IL-1β expression was marginal. In conclusion, the oral probiotic oraCMU demonstrated anti-inflammatory and tissue-protective effects in the peri-implantitis cell model by effectively suppressing the expression of inflammatory and tissue-destructive factors. These findings provide foundational scientific evidence that oraCMU can be utilized as a safe and effective probiotic-based strategy for the prevention and adjunctive treatment of peri-implantitis.