Microbiological and clinical effects of enamel matrix derivative and sustained-release micro-spherical minocycline application as an adjunct to non-surgical therapy in peri-implant mucosal inf ammation
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Objectives: The purpose of this study was to compare the microbial and clinical effects of mechanical debridement (MD) alone or in combination with the application of enamel matrix derivative (EMD) and sustained-release micro-spherical minocycline (MSM) for treatment of peri-implant mu-cosal inf ammation (PIMI).Materials and Methods: Subjects with at least one implant with PIMI were included and divided into control and two different test groups. In all three groups, MD was performed. In the MSM group, following MD, MSM was placed subgingivally around the implants. In the EMD group, after MD, EMD was placed in the sulcus around the implants. Sampling of peri-implant crevicular f uid for microbial analysis with real-time polymerase chain reaction and recording of probing depth (PD) and bleeding on probing (BOP) were performed prior to as well as two weeks and three months after treatment. Median values and interquartile range were estimated for each variable during the various assessment intervals of the study.Results: In all groups, at two weeks and three months, the counts of Porphyromonas gingivalis decreased signif cantly compared to baseline. Levels of P. gingivalis were signif cantly reduced in MSM (P<0.001) and EMD (P=0.026) groups compared to the control group. Also, clinical parameters improved signif cantly at two weeks and three months. Reduction of PD was signif cant in MSM (P<0.001) and EMD (P<0.001) groups. The decrease in BOP in the MSM, EMD, and control groups was 60%, 50%, and 20%, respectively.Conclusion: The use of MSM and EMD can be an adjunctive treatment for management of PIMI and improves clinical parameters and reduces P. gingivalis burden three months after treatment.
Abstract
Objectives: The purpose of this study was to compare the microbial and clinical effects of mechanical debridement (MD) alone or in combination with the application of enamel matrix derivative (EMD) and sustained-release micro-spherical minocycline (MSM) for treatment of peri-implant mu-cosal inf ammation (PIMI).Materials and Methods: Subjects with at least one implant with PIMI were included and divided into control and two different test groups. In all three groups, MD was performed. In the MSM group, following MD, MSM was placed subgingivally around the implants. In the EMD group, after MD, EMD was placed in the sulcus around the implants. Sampling of peri-implant crevicular f uid for microbial analysis with real-time polymerase chain reaction and recording of probing depth (PD) and bleeding on probing (BOP) were performed prior to as well as two weeks and three months after treatment. Median values and interquartile range were estimated for each variable during the various assessment intervals of the study.Results: In all groups, at two weeks and three months, the counts of Porphyromonas gingivalis decreased signif cantly compared to baseline. Levels of P. gingivalis were signif cantly reduced in MSM (P<0.001) and EMD (P=0.026) groups compared to the control group. Also, clinical parameters improved signif cantly at two weeks and three months. Reduction of PD was signif cant in MSM (P<0.001) and EMD (P<0.001) groups. The decrease in BOP in the MSM, EMD, and control groups was 60%, 50%, and 20%, respectively.Conclusion: The use of MSM and EMD can be an adjunctive treatment for management of PIMI and improves clinical parameters and reduces P. gingivalis burden three months after treatment.
참고문헌 (39)
- Misch CE, "Implant success, survival, and failure: the International Congress of Oral Implantologists (ICOI) Pisa Consensus Conference", Implant Dent, 17, pp.5~15 (2008)
- Esposito M, "Biological factors contributing to failures of osseointegrated oral implants. (II). Etiopathogenesis", Eur J Oral Sci, 106, pp.721~764 (1998)
- Pjetursson BE, "A systematic review of the survival and complication rates of fi xed partial dentures (FPDs) after an observation period of at least 5years", Clin Oral Implants Res, 15, pp.667~676 (2004)
- Heitz-Mayfi eld LJ, "Peri-implant diseases: diagnosis and risk indicators", J Clin Periodontol, 35(8 Suppl), pp.292~304 (2008)
- Apse P, "Microbiota and crevicular fl uid collagenase activity in the osseointegrated dental implant sulcus: a comparison of sites in edentulous and partially edentulous patients", J Periodontal Res, 24, pp.96~105 (1989)
- Serino G, "Peri-implantitis in partially edentulous patients:association with inadequate plaque control", Clin Oral Implants Res, 20, pp.169~174 (2009)
- Loe H, "Experimental gingivitis in man", J Periodontol, 36, pp.177~187 (1965)
- Renvert S, "Non-surgical treatment of peri-implant mucositis and peri-implantitis: a literature review", J Clin Periodontol, 35(8 Suppl), pp.305~315 (2008)
- Ericsson I, "The effect of antimicrobial therapy on periimplantitis lesions. An experimental study in the dog", Clin Oral Implants Res, 7, pp.320~328 (1996)
- Mombelli A, "Treatment of periimplantitis by local delivery of tetracycline. Clinical, microbiological and radiological results", Clin Oral Implants Res, 12, pp.287~294 (2001)
- Büchter A, "Sustained release of doxycycline for the treatment of peri-implantitis:randomised controlled trial", Br J Oral Maxillofac Surg, 42, pp.439~444 (2004)
- Williams RC, "Treatment of periodontitis by local administration of minocycline microspheres: a controlled trial", J Periodontol, 72, pp.1535~1544 (2001)
- Leung WK, "Microfl ora cultivable from minocycline strips placed in persisting periodontal pockets", Arch Oral Biol, 50, pp.39~48 (2005)
- Hammarström L, "Periodontal regeneration in a buccal dehiscence model in monkeys after application of enamel matrix proteins", J Clin Periodontol, 24, pp.669~677 (1997)
- Casati MZ, "Enamel matrix derivative and bone healing after guided bone regeneration in dehiscence-type defects around implants. A histomorphometric study in dogs", J Periodontol, 73, pp.789~796 (2002)
- Spahr A, "Effect of the enamel matrix derivative Emdogain on the growth of periodontal pathogens in vitro", J Clin Periodontol, 29, pp.62~72 (2002)
- Gutierrez MA, "Evaluation of enamel matrix derivative as an adjunct to non-surgical periodontal therapy", J Clin Periodontol, 30, pp.739~745 (2003)
- Haffajee AD, "Microbiology of periodontal diseases:introduction", Periodontol 2000, 38, pp.9~12 (2005)
- Kadkhodazadeh M, "Evaluation of peri-implant tissue health using a scoring system", J Implant Adv Clin Dent, 4, pp.51~57 (2012)
- "Place unknown: Geoffrey C. Urbaniak and Scott Plous 1997-2015"[URL]
- Lang NP, "Consensus statements and recommended clinical procedures regarding implant survival and complications", Int J Oral Maxillofac Implants, 19(Suppl), pp.150~154 (2004)
- Mombelli A, "The microbiota associated with successful or failing osseointegrated titanium implants", Oral Microbiol Immunol, 2, pp.145~151 (1987)
- Schou S, "Microbiology of ligature-induced marginal inflammation around osseointegrated implants and ankylosed teeth in cynomolgus monkeys (Macaca fascicularis)", Clin Oral Implants Res, 7, pp.190~200 (1996)
- Holt SC, "Virulence factors of Porphyromonas gingivalis", Periodontol 2000, 20, pp.168~238 (1999)
- Lang NP, "Biological complications with dental implants: their prevention, diagnosis and treatment", Clin Oral Implants Res, 11(Suppl 1), pp.146~155 (2000)
- Kato T, "Bactericidal efficacy of carbon dioxide laser against bacteria-contaminated titanium implant and subsequent cellular adhesion to irradiated area", Lasers Surg Med, 23, pp.299~309 (1998)
- Sahm N, "Non-surgical treatment of peri-implantitis using an air-abrasive device or mechanical debridement and local application of chlorhexidine: a prospective, randomized, controlled clinical study", J Clin Periodontol, 38, pp.872~878 (2011)
- Park JB, "In vitro analysis of the effi cacy of ultrasonic scalers and a toothbrush for removing bacteria from resorbable blast material titanium disks", J Periodontol, 84, pp.1191~1198 (2013)
- Renvert S, "Treatment of incipient peri-implant infections using topical minocycline microspheres versus topical chlorhexidine gel as an adjunct to mechanical debridement", J Int Acad Periodontol, 6(4 Suppl), pp.154~159 (2004)
- Persson GR, "Antimicrobial therapy using a local drug delivery system (Arestin) in the treatment of peri-implantitis. I: microbiological outcomes", Clin Oral Implants Res, 17, pp.386~393 (2006)
- Renvert S, "Topical minocycline microspheres versus topical chlorhexidine gel as an adjunct to mechanical debridement of incipient peri-implant infections:a randomized clinical trial", J Clin Periodontol, 33, pp.362~369 (2006)
- Gestrelius S, "In vitro studies on periodontal ligament cells and enamel matrix derivative", J Clin Periodontol, 24, pp.685~692 (1997)
- Bosshardt DD, "Biological mediators and periodontal regeneration:a review of enamel matrix proteins at the cellular and molecular levels", J Clin Periodontol, 35(8 Suppl), pp.87~105 (2008)
- Wennström JL, "Some effects of enamel matrix proteins on wound healing in the dento-gingival region", J Clin Periodontol, 29, pp.9~14 (2002)
- Mombelli A, "Enamel matrix proteins and systemic antibiotics as adjuncts to nonsurgical periodontal treatment: clinical effects", J Clin Periodontol, 32, pp.225~230 (2005)
- Sculean A, "Treatment of peri-implantitis with EDTA decontamination and application of an enamel matrix protein derivate-a report of 3 case", Periodontal Practice Today, 1, pp.237~245 (2004)
- Boutaga K, "Comparison of real-time PCR and culture for detection of Porphyromonas gingivalis in subgingival plaque samples", J Clin Microbiol, 41, pp.4950~4954 (2003)
- Boutaga K, "The additional value of real-time PCR in the quantitative detection of periodontal pathogens", J Clin Periodontol, 33, pp.427~433 (2006)
- Sakamoto M, "Rapid detection and quantification of five periodontopathic bacteria by real-time PCR", Microbiol Immunol, 45, pp.39~44 (2001)