본문 바로가기
KERD
논문KCI 등재

Heat-inactivated Weissella cibaria CMU Downregulates the mRNA Expression of Proinflammatory Cytokines and Matrix Metalloproteinases in Porphyromonas gingivalis Lipopolysaccharide-Stimulated Human Gingival Fibroblasts

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

초록

Weissella cibaria CMU (oraCMU) is reported as one of the probiotics that promote oral health. Various periodontal pathogens, including Porphyromonas gingivalis (P. gingivalis), can cause periodontal tissue destruction and periodontal disease. P. gingivalis infection in human gingival fibroblasts (HGFs) induces the expression of proinflammatory cytokines and enhances the production of matrix metalloproteinases (MMPs). The present study investigated the inhibitory effects of heat-inactivated oraCMU on the mRNA expression of proinflammatory cytokines and MMPs in P. gingivalis LPS-stimulated HGFs. Cell viability was performed using the MTS assay. The mRNA expression of proinflammatory cytokines, such as interleukin-1β (IL-1β) and, IL-6, tumor necrosis factor-α (TNF-α), and MMPs, such as MMP-1 and MMP-3, was examined by reverse transcription-quantitative PCR (RT-qPCR). Heat-inactivated oraCMU dose-dependently inhibited the mRNA expression of IL-1β, IL-6, and TNF-α in P. gingivalis LPS-stimulated HGFs. Also, the mRNA expression of MMP-1 and MMP-3 dose-dependently decreased. These results suggest that heat-inactivated oraCMU could be an effective probiotic for preventing periodontitis.

Abstract

Weissella cibaria CMU (oraCMU) is reported as one of the probiotics that promote oral health. Various periodontal pathogens, including Porphyromonas gingivalis (P. gingivalis), can cause periodontal tissue destruction and periodontal disease. P. gingivalis infection in human gingival fibroblasts (HGFs) induces the expression of proinflammatory cytokines and enhances the production of matrix metalloproteinases (MMPs). The present study investigated the inhibitory effects of heat-inactivated oraCMU on the mRNA expression of proinflammatory cytokines and MMPs in P. gingivalis LPS-stimulated HGFs. Cell viability was performed using the MTS assay. The mRNA expression of proinflammatory cytokines, such as interleukin-1β (IL-1β) and, IL-6, tumor necrosis factor-α (TNF-α), and MMPs, such as MMP-1 and MMP-3, was examined by reverse transcription-quantitative PCR (RT-qPCR). Heat-inactivated oraCMU dose-dependently inhibited the mRNA expression of IL-1β, IL-6, and TNF-α in P. gingivalis LPS-stimulated HGFs. Also, the mRNA expression of MMP-1 and MMP-3 dose-dependently decreased. These results suggest that heat-inactivated oraCMU could be an effective probiotic for preventing periodontitis.

참고문헌 (27)

  1. Bodet C, "Actinobacillus actinomycetemcomitans lipopolysaccharide regulates matrix metalloproteinase, tissue inhibitors of matrix metalloproteinase, and plasminogen activator production by human gingival fibroblasts : A potential role in connective tissue destruction", J Cell Physiol, 212(1), pp.189~194 (2007)[DOI]
  2. Wen WC, "Epigallocatechin-3-Gallate Attenuates Porphyromonas gingivalis Lipopolysaccharide-Enhanced Matrix Metalloproteinase-1 Production Through Inhibition of Interleukin-6 in Gingival Fibroblasts", J Periodontol, 85(6), pp.868~875 (2014)[DOI]
  3. 나희삼, "Plasminogen Activator Inhibitor Type 1 Expression Induced by Lipopolysaccharide of Porphyromonas gingivalis in Human Gingival Fibroblast", The Journal of Microbiology, 52(2), pp.154~160 (2014), 한국미생물학회[DOI]
  4. Howard KC, "Porphyromonas gingivalis : where do we stand in our battle against this oral pathogen?", RSC Med Chem, 12(5), pp.666~704 (2021)[DOI]
  5. Herath TDK, "Tetra-and Penta-Acylated Lipid A Structures of Porphyromonas gingivalis LPS Differentially Activate TLR4-Mediated NF-κB Signal Transduction Cascade and Immuno-Inflammatory Response in Human Gingival Fibroblasts", PLoS ONE, 8(3), pp.e58496~ (2013)[DOI]
  6. Franco C, "Matrix Metalloproteinases as Regulators of Periodontal Inflammation", Int J Mol Sci, 18(2), pp.440~ (2017)[DOI]
  7. Futamura-Masuda M, "Effect of Asiasarum Root Extract and Its Constituents on Interleukin-1β-Stimulated Matrix Metalloproteinase-1 Secretion from Human Gingival Fibroblasts", Biol Pharm Bull, 39(5), pp.823~831 (2016)[DOI]
  8. Zhou J, "Porphyromonas gingivalis affects host collagen degradation by affecting expression, activation, and inhibition of matrix metalloproteinases", J Periodontal Res, 41(1), pp.47~54 (2006)[DOI]
  9. Feldman M, "Porphyromonas gingivalis-mediated shedding of extracellular matrix metalloproteinase inducer(EMMPRIN)by oral epithelial cells : a potential role in inflammatory periodontal disease", Microbes Infect, 13, pp.1261~1269 (2011)[DOI]
  10. Chowdhury NU, "Matrix Metalloproteinases(MMP), a Major Responsible Downstream Signaling Molecule for Cellular Damage-A Review", Mol Enzymol Drug Targets, 2(2), pp.1~10 (2016)[DOI]
  11. Yiemwattana I, "Morus alba Stem Extract Suppresses Matrix Metalloproteinases(MMP)-1, MMP-9, and Tissue Inhibitors of Metalloproteinase(TIMP)-1 Expression via Inhibition of IκBα Degradation Induced by Porphyromonas gingivalis LPS Signal in THP-1Cells", Eur J Dent, 13(02), pp.229~234 (2019)[DOI]
  12. Dreier R, "Pro-MMP-9 is a specific macrophage product and is activated by osteoarthritic chondrocytes via MMP-3 or a MT1-MMP", MMP-13 cascade, 2004, pp.297(2), 303~312
  13. Domeij H, "Signal pathways involved in the production of MMP-1 and MMP-3 in human gingival fibroblasts", Eur J Oral Sci, 110(4), pp.302~306 (2002)[DOI]
  14. Boukpessi T, "The effect of stromelysin-1(MMP-3)on non-collagenous extracellular matrix proteins of demineralized dentin and the adhesive properties of restorative resins", Biomaterials, 29(33), pp.4367~4373 (2008)[DOI]
  15. Herath TDK, "The expression and regulation of matrix metalloproteinase-3 is critically modulated by Porphyromonas gingivalis lipopolysaccharide with heterogeneous lipid A structures in human gingival fibroblasts", BMC Microbiol, 13(1), pp.73~ (2013)[DOI]
  16. Huang C, "Eldecalcitol Inhibits LPS-Induced NLRP3 Inflammasome-Dependent Pyroptosis in Human Gingival Fibroblasts by Activating the Nrf2", HO-1 Signaling Pathway, 2020, pp.14, 4901~4913
  17. Naruishi K, "Biological effects of interleukin-6 on Gingival Fibroblasts : Cytokine regulation in periodontitis", J Cell Physiol, 233(9), pp.6393~6400 (2018)[DOI]
  18. Li X, "Hyperresponsiveness of human gingival fibroblasts from patients with aggressive periodontitis against bacterial lipopolysaccharide", Exp Ther Med, 21(5), pp.417~ (2021)[DOI]
  19. Nakagawa M, "Long-term exposure to butyric acid induces excessive production of matrix metalloproteases in human gingival fibroblasts", Arch Oral Biol, 123, pp.105035~ (2021)[DOI]
  20. Da Rocha HAJ, "Local Drug Delivery Systems in the Treatment of Periodontitis : A Literature Review", J Int Acad Periodontol, 17(3), pp.82~90 (2015)
  21. Maquera-Huacho PM, "Eriodictyol Suppresses Porphyromonas gingivalis-Induced Reactive Oxygen Species Production by Gingival Keratinocytes and the Inflammatory Response of Macrophages", Front Oral Health, 3, pp.847914~ (2022)[DOI]
  22. Kong L, "Theaflavins inhibit pathogenic properties of P. gingivalis and MMPs production in P. gingivalis-stimulated human gingival fibroblasts", Arch Oral Biol, 60(1), pp.12~22 (2015)[DOI]
  23. Jang HJ, "Comparative Study on the Characteristics of Weissella cibaria CMU and Probiotic Strains for Oral Care", Molecules, 21(12), pp.1752~ (2016)[DOI]
  24. Kim MJ, "Weissella cibaria CMU exerts an anti-inflammatory effect by inhibiting Aggregatibacter actinomycetemcomitans-induced NF-κB activation in macrophages", Mol Med Rep, 22(5), pp.4143~4150 (2020)[DOI]
  25. 강미선, "Effect of Weissella cibaria on Fusobacterium nucleatum-induced Interleukin-6 and Interleukin-8 Production in KB Cells", Journal of Bacteriology and Virology, 41(1), pp.9~18 (2011), 대한미생물학회
  26. Schmittgen TD, "Analyzing real-time PCR data by the comparative C(T) method", Nature Protocols, 3(6), pp.1101~1108 (2008)[DOI]
  27. Domeij H, "Signal pathways involved in the production of MMP-1 and MMP-3 in human gingival fibroblasts", Eur J Oral Sci, 110(4), pp.302~306 (2002)[DOI]

관련 논문AI추천

이 간행물의 다른 논문 (10건)