세균배지의 정도관리에 관한 검토

Abstract

To estirnate the accuracy, efficiency and reproducibility of the test results of clinical bacteriological laboratory, we evaluated the performance test and sterility test for the media and identification reagents from May 1997 through December 1997. The results were; 1. By the sterility test for media, no contaminated organisms were isolated. 2. By the performance test of the enrichment media, selective differential media, biochemical meida and identification reagents for the positive and negative control organisms, the acceptable data were yielded. 3. It should be boiled for 60 minutes that the best effective result of MacConkey agar, xylose lysine desoxycholate(XLD) agar and salmonelIashigella(S-S) agar can be obtained and for the growth on the media bacterial colony count must be more than 3 x 103-4/mL 4. The MacConkey agar showed the best growth of the three selective medium and XLD agar showed better detection for salmonella than S-S agar. 5. It showed that UV -light and dry media make the bacterial growth to be inhibited. For the best isolation and identification of the bacteria from the samples, we should always perform the quality control adivites in dinical bacteriological laboratory .