지방산 분석을 이용한 Vibrio속 세균의 동정

Abstract

Isolated from Nam Hae coastal waters, three gram-negative Vibrio were compared with T the type spcies Vibrio parahaemolyticus ATCC17802T, Vibrio alginolyticus 2472 (ATCCI77491T), Vibrio vulnificus ATCC27562T with regard to physio-biochemical characters. The celluar fatty-acid compositions of the three Vibrios spp. were also compared by examing their taxonomic markers and the isolates were gram-negative curved rods. and showed positive characters on the nitrate reduction, oxi없se and glucose fermentation tests. Also the isolates grew on TCBS agar plates, in alkaline peptone water at 35ºC, and on nutriant agar plates at 40ºC . Two of the three isolates were identified as V. parahaemolyticus and one was V. algonolyticus by using the API ID 32 GN kit and physio-biochemical characters. The isolates contained of 16:1w7c(21-38%), 16:0(16-32%), 18:1w7c(8-26), major cellular fatty-acid compositions the same as the Vibrio type strains. Asa result of thls study, we can clearly see that physio-biochemical characters and cellular fatty-acid composition provide useful tools for identification of bacteria, especially in the study of genus Vibrio.