Vibrio vulnificus의 생물형, 혈청형, Repetitive Sequence Based PCR 양상 및 항균제 감수성 시험

Abstract

This study was perfonned to provide c1arity on the environmental distribution of Vibrio vulnificus oysters, biological characteristics, distribution of biotypes and serotypes, the detection of the cytolysin gene by PCR, repetitive sequence-based PCR (rep-PCR) analysis and antimicrobialsusceptibility of the isolates from oysters and patients in Korea. V. vulnificus was isolated from 67 (38.5%) of the 175 samples of oysters collected at a shoreline in the Kunsan city area in July and August, 2002. Oyster and patient isolates had similar biochemical characteristics, i.e., all were positive on an ONPG test, but not for sucrose. When comparing the identification of V. vulnificus obtained with the API 20E assay and serotyping, 87 API 20E profiles with both a separation test on the colony of TCBS agar and an ONPG test were obtained with 84 profiles (96.6%), but three isolates were not identifiable with the API 20E system. API 20E code numbers showed 13 different biotypes in 87 isolates. The lrighest rate of 48 (55.2%) isolates of API 20E code numbers was obtained with code No. 5146105 (biotype IV). Oyster isolates belonged to thirteen serotypes and patient strains belonged to 9 serotypes. Prevalent serotypes were O12, and O4A, 16 (18.6%) and 13 (15.1 %) from oysters, and 52 (67.5%) and 7 (9.1%) of O4A and O4AB from patients, respectively. 163 isolates of V. vulnificus cytolysin gene from oysters and patients were positive by PCR. 30 V. vulnificus isolates were divided into 5 genotypes using rep-PCR analysis. Only three bands have all of the isolates from the oysters by rep-PCR. on an antimicrobial susceptibility test they were susceptible to tetracycline, chloramphenicol and ciprofloxacin, but none were susceptible to colistin.