임상검체에서 분리한 Vibrio parahaemolyticus의 생물학적 성상, 혈청형, toxR 유전자의 검출 및 시험관내 항균제 감수성

Abstract

Vibrio parahaemolyticus is one of the most important food-bome pathogens in Korea, Japan, and other cuntries with long coastlines. The aim of this study was to determine the biochemical characteristics, serovar, antimicrobial, susceptibility and possession of toxR gene of the 46 V. parahaemolyticus isolates from clinical specimens. Biochemical characteristics of the these were typical, but 28 % and 4 % of isolates showed negative to Simmon citrate and omithine decarboxylase test, respectively. Six kinds of serovars were detected in the isolates. Serovar 03:K6 were most prevalent i.e., 85%. Other serovar including 03:K9, 03:K55, 03:K56, 03:KUT and R were very rare. Most of the isolates were resistant to ampicillin, carbenicillin and cefazolin, but none were resistant to most of the other antimicrobial agents including tetracycline, which is the drug of choice for the treatment of Vibrio infection. V. parahaemolyticus toxR gene was detected from all of the 46 isolates. It is concluded that the biochemical characteristics of most of the V. parahaemolyticlα of isolated Wonkwang University Hospital are typical, that prevalent serovar are 03:K6, and that isolates with antimicrobial resistance with the exception ampicillin, carbenicillin and cefazolin are very rare. Also toxR gene detection by the PCR test can be used for rapid identification of the V. parahaemolyticus in the clinical laboratory.