Rhynchosia nulubilis and Rhynchosia volubilis Ethanol Extracts Upregulate the Expression of Genes Related to Melanogenesis in Melan-a Cells
0
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
To determine the molecular mechanisms underlying the melanogenic effects of black soy bean ethanol extracts, including Rhynchosia nulubilis bean ethanol extract (RNBEE), R. nulubilis leaf ethanol extract (RNLEE), R. volubilis bean ethanol extract (RVBEE), and R. volubilis leaf ethanol extract (RVLEE), we evaluated their effects on tyrosinase, TRP-1, TRP- 2, and MITF-M gene expression at mRNA and protein levels. Compared to the control group at 50 μg/mL, tyrosinase and TRP-1 mRNA expression was significantly increased by RNLEE, RVBEE, and RVLEE treatment, whereas RNBEE significantly increased TRP-1 mRNA expression but did not affect tyrosinase mRNA expression. RNLEE significantly increased TRP-2 and MITF-M mRNA expression; RVLEE significantly increased MITF-M mRNA expression. Compared to the control group at 50 μg/mL, RNBEE, RNLEE, and RVLEE significantly increased the protein levels of tyrosinase, TRP-1, TRP-2, and MITF-M. RVBEE significantly increased the protein levels of tyrosinase, TRP-1, and MITF-M, but did not affect that of TRP-2 protein. We conclude that the potential mechanism underlying the melanogenic effects of black soybean ethanol extracts is upregulation of tyrosinase mRNA and protein expression along with an increase in tyrosinase activity. The increase in mRNA and protein levels of TRP-1, TRP-2, or MITF-M by RNEE and RVEE might also be contribute to the increase in melanin synthesis.
Abstract
To determine the molecular mechanisms underlying the melanogenic effects of black soy bean ethanol extracts, including Rhynchosia nulubilis bean ethanol extract (RNBEE), R. nulubilis leaf ethanol extract (RNLEE), R. volubilis bean ethanol extract (RVBEE), and R. volubilis leaf ethanol extract (RVLEE), we evaluated their effects on tyrosinase, TRP-1, TRP- 2, and MITF-M gene expression at mRNA and protein levels. Compared to the control group at 50 μg/mL, tyrosinase and TRP-1 mRNA expression was significantly increased by RNLEE, RVBEE, and RVLEE treatment, whereas RNBEE significantly increased TRP-1 mRNA expression but did not affect tyrosinase mRNA expression. RNLEE significantly increased TRP-2 and MITF-M mRNA expression; RVLEE significantly increased MITF-M mRNA expression. Compared to the control group at 50 μg/mL, RNBEE, RNLEE, and RVLEE significantly increased the protein levels of tyrosinase, TRP-1, TRP-2, and MITF-M. RVBEE significantly increased the protein levels of tyrosinase, TRP-1, and MITF-M, but did not affect that of TRP-2 protein. We conclude that the potential mechanism underlying the melanogenic effects of black soybean ethanol extracts is upregulation of tyrosinase mRNA and protein expression along with an increase in tyrosinase activity. The increase in mRNA and protein levels of TRP-1, TRP-2, or MITF-M by RNEE and RVEE might also be contribute to the increase in melanin synthesis.
참고문헌 (14)
- Choung MG, "Isolation and determination of anthocyanins in seed coats of black soybean (Glycine max (L.) Merr.)", J Agric Food Chem, 49, pp.5848~5851 (2001)
- Fuse N, "Identification of a melanocyte-type promoter of the microphthalmia- associated transcription factor gene", Biochem Biophys Res Commun, 219, pp.702~707 (1996)
- Hoh JF, "Effects of dibutyryl cyclic AMP, ouabain, and xanthine derivatives on cross bridge kinetics in rat cardiac muscle", Circ Res, 68, pp.702~713 (1991)
- 홍성희, "Melanogenesis-Promoting Effects of Rhynchosia nulubilis and Rhynchosia volubilis Ethanol Extracts in Melan-a Cells", 한국독성학회지, 32(2), pp.141~147 (2016), 한국독성학회[DOI]
- Hunt G, "Alpha-melanocyte stimulating hormone and its analogue Nle4DPhe7 alpha-MSH affect morphology, tyrosinase activity and melanogenesis in cultured human melanocytes", J Cell Sci, 107, pp.205~211 (1994)
- Jeng TL, "Comparisons of flavonoids and anti-oxidative activities in seed coat, embryonic axis and cotyledon of black soybeans", Food Chem, 123, pp.1112~1116 (2010)
- Lee HD, "Manassantin A inhibits cAMP-induced melanin production by down-regulating the gene expressions of MITF and tyrosinase in melanocytes.", Exp Dermatol, 20, pp.761~763 (2011)
- 이재순, "Effects of Soybean Methanolic Extract on the Expression of Melanogenic Genes", 대한미용학회지, 8(4), pp.219~225 (2012), 대한미용학회
- 이재순, "Promoting Effects of Soybean Methanol Extract on the Melanogenesis and Tyrosinase Activity", 대한미용학회지, 9(4), pp.8~14 (2013), 대한미용학회[DOI]
- Naish-Byfield S, "Tyrosinase kinetics: failure of acceleration in oxidation of ring-blocked monohydric phenol substrate", Pigment Cell Res, 11, pp.94~97 (1998)
- Park HY, "MITF mediates cAMP-induced protein kinase C-β expression in human melanocytes", Biochem J, 395, pp.571~578 (2006)
- Udono T, "Structural organization of the human microphthalmia-associated transcription factor gene containing four alternative promoters", Biochim Biophys Acta, 1491, pp.205~219 (2000)
- Yasumoto K, "Functional analysis of microphthalmia-associated transcription factor in pigment cell-specific transcription of the human tyrosinase family genes", J Biol Chem, 272, pp.503~509 (1997)
- Yoshida K, "Structural analysis and measurement of anthocyanins from colored seed coats of Vigna, Phaseolus, and Glycine Legumes", Biosci Biotech Biochem, 60, pp.589~593 (1996)