Enhancement of Melanin Synthesis by the Branch Extracts of Vaccinium oldhamii through Activating Tyrosinase Activity in B16F10 Melanoma Cells
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초록
This study was conducted to investigate the effect of branch extracts of Vaccinium oldhamii (VOB) on melanin synthesis in B16F10 cells. VOB promoted melanin production in absence or presence of α-melanocyte-stimulating hormone (α-MSH) in B16F10 cells. However, VOB did not affect the expression of tyrosinase and TRP-1 associated with melanin synthesis at the mRNA and protein levels in B16F10. But, VOB decreased TRP-2 protein level and induced tyrosinase activation in B16F10 cells. Inhibition of tyrosinase activity and tyrosinase knockdown attenuated VOB-mediated melanin synthesis. In conclusion, VOB may stimulate melanin synthesis through activating tyrosinase activity.
Abstract
This study was conducted to investigate the effect of branch extracts of Vaccinium oldhamii (VOB) on melanin synthesis in B16F10 cells. VOB promoted melanin production in absence or presence of α-melanocyte-stimulating hormone (α-MSH) in B16F10 cells. However, VOB did not affect the expression of tyrosinase and TRP-1 associated with melanin synthesis at the mRNA and protein levels in B16F10. But, VOB decreased TRP-2 protein level and induced tyrosinase activation in B16F10 cells. Inhibition of tyrosinase activity and tyrosinase knockdown attenuated VOB-mediated melanin synthesis. In conclusion, VOB may stimulate melanin synthesis through activating tyrosinase activity.
참고문헌 (28)
- Sumathy Babitha, "Potential of Cassia alata Leaf Extract in Inducing Differentiation and Migration of Mouse Melanoblasts", Biotechnology and Bioprocess Engineering, 15(6), pp.1071~1076 (2010), 한국생물공학회
- Chaabane, F, "Phytochemical potential of Daphne gnidium in inhibiting growth of melanoma cells and enhancing melanogenesis of B16-F0 melanoma", Cell Biochem. Funct, 31, pp.460~467 (2013)
- Chaabane, F, "Chloroform leaf extract of Daphne gnidium inhibits growth of melanoma cells and enhances melanogenesis of B16-F0 melanoma", S. Afr. J. Bot, 90, pp.80~86 (2014)
- del Marmol, V, "Tyrosinase and related proteins in mammalian pigmentation", FEBS Lett, 381, pp.165~168 (1996)
- Eller, M.S, "DNA damage and melanogenesis", Nature, 372, pp.413~414 (1994)
- Fang, D, "Regulation of tyrosinaserelated protein-2 (TYRP2) in human melanocytes: relationship to growth and morphology", Pigment Cell Melanoma Res, 14, pp.132~139 (2001)
- Fistarol, S.K, "Disorders of pigmentation", J. Dtsch. Dermatol. Ges, 8, pp.187~201 (2010)
- Han, E, "Melanogenesis inhibitory effect of aerial part of Pueraria thunbergiana in vitro and in vivo", Arch. Dermatol. Res, 307, pp.57~72 (2015)
- Hearing, V, "Enzymatic control of pigmentation in mammals", FASEB J, 5, pp.2902~2909 (1991)
- Hearing, V, "Mammalian tyrosinase - the critical regulatory control point in melanocyte pigmentation", Int. J. Biochem, 19, pp.1141~1147 (1987)
- Hirotoshi, T, "Antioxidant activities and anticancer cell proliferation properties of Natsuhaze (Vaccinium oldhamii Miq.), Shashanbo (V. bracteatum Thunb.) and Blueberry cultivars", Planta, 2, pp.57~71 (2013)
- Kameyama, K, "The expression of tyrosinase, tyrosinase-related proteins 1 and 2 (TRP1 and TRP2), the silver protein, and a melanogenic inhibitor in human melanoma cells of differing melanogenic activities", Pigment Cell Melanoma Res, 8, pp.97~104 (1995)
- Kobayashi, T, "Tyrosinase related protein 1 (TRP1) functions as a DHICA oxidase in melanin biosynthesis", EMBO J, 13, pp.5818~5825 (1994)
- Jae Hyeok Lee, "Acetylcholinesterase Inhibitors from the Twigs of Vaccinium oldhami Miquel", Archives of Pharmacal Research, 27(1), pp.53~56 (2004), 대한약학회
- Moreira, C.G, "Hyperpigmentant activity of leaves and flowers extracts of Pyrostegia venusta on murine B16-F10 melanoma", J. Ethnopharmacol, 141, pp.1005~1011 (2012)
- Niu, C, "Upregulation of melanogenesis and tyrosinase activity: Potential agents for vitiligo", Molecules, 22, pp.1303~ (2017)
- 오순자, "제주도에 자생하는 피자식물의 항산화활성과 α-amylase 저해활성 검색", 한국자원식물학회지, 22(1), pp.71~77 (2009), 한국자원식물학회
- Oh, T.I, "Plumbagin suppresses α-MSH-induced melanogenesis in B16F10 mouse melanoma cells by inhibiting tyrosinase activity", Int. J. Mol. Sci, 18, pp.320~329 (2017)
- 박수빈, "Anticancer Activity of the Branch Extracts from Vaccinium oldhamii through Cyclin D1 Proteasomal Degradation in Human Cancer Cells", 한국자원식물학회지, 31(3), pp.218~227 (2018), 한국자원식물학회[DOI]
- Park, S.H, "Terrein: a new melanogenesis inhibitor and its mechanism", Cell. Mol. Life Sci, 61, pp.2878~2885 (2004)
- Schiaffinom, M.V, "Signaling pathways in melanosome biogenesis and pathology", Int. J. Biochem. Cell Biol, 42, pp.1094~1104 (2010)
- Skandrani, I, "Chloroform extract from Moricandia arvensis inhibits growth of B16-F0 melanoma cells and promotes differentiation in vitro", Cell Prolif, 43, pp.471~479 (2010)
- Tuerxuntayi, A, "Kaliziri extract upregulates tyrosinase, TRP-1, TRP-2 and MITF expression in murine B16 melanoma cells", BMC Complement. Altern. Med, 14, pp.1~9 (2014)
- Xu, P, "Effects of aqueous extracts of Ecliptae herba, Polygoni multiflori radix praeparata and Rehmanniae radix praeparata on melanogenesis and the migration of human melanocytes", J. Ethnopharmacol, 195, pp.89~95 (2017)
- Yamaguchi, Y, "The regulation of skin pigmentation", J. Biol. Chem, 282, pp.27557~27561 (2007)
- Ye, Y, "Involvement of p38MAPK signaling pathway in the anti-melanogenic effect of San-bai-tang, a Chinese herbal formula, in B16 cells", J. Ethnopharmacol, 132, pp.533~535 (2010)
- Yokoyama, K, "Cloning of the human DOPAchrome tautomerase", tyrosinaserelated protein 2 gene and identification of two regulatory regions required for its pigment cell-specific expression, 1994, pp.269, 27080~27087
- Zhou, J, "Alcohol extract from alcohol extract from Vernonia anthelmintica (L.) wild seed enhances melanin synthesis through activation of the p38 MAPK signaling pathway in B16F10 cells and primary melanocytes", J. Ethnopharmacol, 143, pp.639~647 (2012)
