진달래뿌리추출물과 이로부터 분리한 taxifolin-3-O-alpha-L-arabinopyranoside의 항노화 활성
Anti-aging activity of taxifolin-3-O-alpha-L-arabinopyranoside isolated from Rhododendron mucronulatum root extract
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초록
In this experiment, we evaluated the anti-aging activity of Rhododendron mucronulatum root extract and taxifolin-3-O-alp ha-L-arabinopyranoside isolated from it. R. mucronulatum root extract showed half maximal DPPH radical scavenging concen tration at 850 ㎍/㎖ and half maximal elastase inhibitory concentration at 5,100 ㎍/㎖. Among solvent fractions of R.mucronul atum root extract, ethyl acetate fraction showed the highest DPPH radical scavenging concentration at 1.56 ㎍/㎖ and half ma ximal elastase inhibitory concentration at 525 ㎍/㎖. Also, ethyl acetate fraction showed the highest type-1-procollagen biosyn thesis activity. The type-1-procollagen biosynthesis level of taxifolin-3-O-alpha-L-arabinopyranoside was tested in human de rmal fibroblast cells. Results showed that taxifolin-3-O-alpha-L-arabinopyranoside increased type I procollagen biosythesis lev el in a dose-dependent manner. These results indicate R.mucronulatum root extracts and taxifolin-3-O-alpha-L-arabinopyrano side could be used as an anti-aging ingredients for functional cosmetics.
Abstract
In this experiment, we evaluated the anti-aging activity of Rhododendron mucronulatum root extract and taxifolin-3-O-alp ha-L-arabinopyranoside isolated from it. R. mucronulatum root extract showed half maximal DPPH radical scavenging concen tration at 850 ㎍/㎖ and half maximal elastase inhibitory concentration at 5,100 ㎍/㎖. Among solvent fractions of R.mucronul atum root extract, ethyl acetate fraction showed the highest DPPH radical scavenging concentration at 1.56 ㎍/㎖ and half ma ximal elastase inhibitory concentration at 525 ㎍/㎖. Also, ethyl acetate fraction showed the highest type-1-procollagen biosyn thesis activity. The type-1-procollagen biosynthesis level of taxifolin-3-O-alpha-L-arabinopyranoside was tested in human de rmal fibroblast cells. Results showed that taxifolin-3-O-alpha-L-arabinopyranoside increased type I procollagen biosythesis lev el in a dose-dependent manner. These results indicate R.mucronulatum root extracts and taxifolin-3-O-alpha-L-arabinopyrano side could be used as an anti-aging ingredients for functional cosmetics.
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