비타민나무 열매로부터 분리한 Quercetin 3-glucoside-7-rhamnoside 의 항산화 및 주름개선 효과
Antioxidant and Anti-wrinkle Effects of Quercetin 3-Glucoside-7-Rhamnoside Isolated from Hippophae rhamnoides Fruits
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초록
Although many studies have been performed to search materials that regulate skin aging, little is known about the effect of quercetin 3-O-glucoside-7-O-rhamnoside (Q3G7R) from Hippophae rhamnoides (H. rhamnoides )L. fruits. In this study, we identified Q3G7R from H. rhamnoides L. fruits, and then measured the antioxidant activity of Q3G7R by 1,1-diphenyl-2-picryl hydrazyl (DPPH) method and its cytotoxicity in human dermal fibroblasts using sulforhodamine B (SRB) assay. Also, we investigated effect of Q3G7R on procollagen synthesis and TNF-α induced Matrix Metalloproteinase-1 (MMP-1) expression in human dermal fibroblasts. As the results, Q3G7R didn’t exhibit cytotoxicity at 100 μg/mL showing cell viability over 100 % and antioxidant activity with 56.4% at concentration of 1 mg/mL. Procollagen synthesis in human dermal fibrolasts increased about 2 times higher than control by treatment with 1 μg/mL of Q3G7R. TNF-α induced MMP-1 expression was reduced to 85.58 ng/mL by treatment with 1 μg/mL of Q3G7R. Therefore, these results suggest that Q3G7R can be effective antioxidant and antiwrinkle ingredient for cosmetics applications.
Abstract
Although many studies have been performed to search materials that regulate skin aging, little is known about the effect of quercetin 3-O-glucoside-7-O-rhamnoside (Q3G7R) from Hippophae rhamnoides (H. rhamnoides )L. fruits. In this study, we identified Q3G7R from H. rhamnoides L. fruits, and then measured the antioxidant activity of Q3G7R by 1,1-diphenyl-2-picryl hydrazyl (DPPH) method and its cytotoxicity in human dermal fibroblasts using sulforhodamine B (SRB) assay. Also, we investigated effect of Q3G7R on procollagen synthesis and TNF-α induced Matrix Metalloproteinase-1 (MMP-1) expression in human dermal fibroblasts. As the results, Q3G7R didn’t exhibit cytotoxicity at 100 μg/mL showing cell viability over 100 % and antioxidant activity with 56.4% at concentration of 1 mg/mL. Procollagen synthesis in human dermal fibrolasts increased about 2 times higher than control by treatment with 1 μg/mL of Q3G7R. TNF-α induced MMP-1 expression was reduced to 85.58 ng/mL by treatment with 1 μg/mL of Q3G7R. Therefore, these results suggest that Q3G7R can be effective antioxidant and antiwrinkle ingredient for cosmetics applications.
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