Anti-Oxidative, Anti-Inflammatory, and Anti-Wrinkling Activities of Sambucus williamsii Hance Methanol Extracts
0
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Sambucus williamsii Hance traditionally used in oriental herbal medicine to treat bone disease. However, its anti-aging effects as a skin care ingredient has not been fully evaluated. Therefore, we investigated the antioxidant, anti-inflammation, and anti-wrinkling activities of S. williamsii methanol extract in vitro using human keratinocytes, HaCaT cells. The total polyphenol contents of the leaf (SWL), tree (SWT), and seed (SWS) extracts were 48.605, 84.132, and 17.750 mgGAE/g, respectively. The DPPH and ABTS radical scavenging activities of S. williamsii methanol extracts showed dose-dependent anti-oxidant activity. Nitrite production and pro-inflammatory cytokine, interleukin (IL)-6 were reduced by after treatment with SWL extract. In addition, extract caused reduction in nitric oxide by affecting expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 in LPS-activated RAW264.7 murine macrophages. S. williamsii tree extract decreased matrix metalloproteinase (MMP)-1 and increased hydration related gene, AQP-3 expression in TNF-a stimulated HaCaT cells. Furthermore, SWT increased the hyaluronan synthase (HAS)-1, 2, and 3 mRNA levels. From these results, we conclude that S. williamsii methanol extract has excellent antioxidative, anti-inflammatory, and even better anti-wrinkle effects. These results suggest that S. williamsii could be used in functional cosmetics for the prevention or alleviation of skin aging.
Abstract
Sambucus williamsii Hance traditionally used in oriental herbal medicine to treat bone disease. However, its anti-aging effects as a skin care ingredient has not been fully evaluated. Therefore, we investigated the antioxidant, anti-inflammation, and anti-wrinkling activities of S. williamsii methanol extract in vitro using human keratinocytes, HaCaT cells. The total polyphenol contents of the leaf (SWL), tree (SWT), and seed (SWS) extracts were 48.605, 84.132, and 17.750 mgGAE/g, respectively. The DPPH and ABTS radical scavenging activities of S. williamsii methanol extracts showed dose-dependent anti-oxidant activity. Nitrite production and pro-inflammatory cytokine, interleukin (IL)-6 were reduced by after treatment with SWL extract. In addition, extract caused reduction in nitric oxide by affecting expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 in LPS-activated RAW264.7 murine macrophages. S. williamsii tree extract decreased matrix metalloproteinase (MMP)-1 and increased hydration related gene, AQP-3 expression in TNF-a stimulated HaCaT cells. Furthermore, SWT increased the hyaluronan synthase (HAS)-1, 2, and 3 mRNA levels. From these results, we conclude that S. williamsii methanol extract has excellent antioxidative, anti-inflammatory, and even better anti-wrinkle effects. These results suggest that S. williamsii could be used in functional cosmetics for the prevention or alleviation of skin aging.
참고문헌 (34)
- A. Altemini, "Phytochemicals: Extraction, isolatio n, and identification of bioactive compounds from plant extracts", Plants(Basel), 6(4), pp.42~ (2017)
- A. Roy, "Flavonoids a Bioactive Compound from Medici nal Plants and Its Therapeutic Applications", BioMed Res earch International, 2022, pp.1~ (2022)
- A. Sofowora, "The role and place of medicinal plants in the strategies for disea se prevention", African Journal of Traditional, Compleme ntary and Alternative Medicines, 10(5), pp.210~ (2013)
- A. Thouri, "Effect of solvents extraction o n phytochemical components and biological activities of Tunisian date seeds (var. Korkobbi and Arechti)", BMC Complementary Medicine and Therapies, 17(248), pp.1~ (2017)
- B. Hwang, "Antifungal activity of lariciresinol derived fro m Sambucus williamsii and their membrane-active mech anisms in Candida albicans", Biochemical and Biophysical Research Communications, 410(3), pp.489~ (2011)
- B. M. Twaij, "Bioactive secondary meta bolites from plant sources: types, synthesis, and their th erapeutic uses", International Journal of Plant Biology, 13(1), pp.4~ (2022)
- C. A. Rice-Evans, "Structure -antioxidant activity relationships of flavonoids and phe nolic aids", Free Radical Biology and Medicine, 20(7), pp.933~ (1996)
- J. W. Chae, "Antioxidative activity of extra cts from Sambucus silliamsii var. coreana", Korean Journ al of Plant Research, 25(4), pp.363~ (2012)
- C. Novais, "Bioactive and Nutritional Potential of Medicinal and Aromatic Plant (MAP) Seaso ning Mixtures", Molecules, 26(6), pp.1587~ (2021)
- G. Zengin, "Phytochemical Profile and Biologic al Activities of Different Extracts of Three Parts of Paliu rus spina-christi: A Linkage between Structure and Abili ty", Antioxidants, 12(2), pp.255~ (2023)
- H. Choi, "Isolation of (-)-olivil-9'-O-β-D-glucopyranoside from Sam bucus williamsii and its antifungal effects with membra ne-disruptive action", Biochemica et Biophysica Acta, 1828(8), pp.2002~ (2013)
- H. Lv, "Isolation of linoleic acid from Sambucus williamsii seed oil extracted by high pressure fluid and its antioxd iant, antiglcemic, hypolipidemic activities", International Journal of Food Engineering, 11(3), pp.383~ (2015)
- H. H. Xiao, "The lignan-rich fraction f rom Sambucus Williamsii Hance ameliorates dyslipidemi a and insulin resistance and modulates gut microbiota c omposition in ovariectomized rats", Biomedicine & Phar macotherapy, 137, pp.111372~ (2021)
- H. H. Xiao, "Bone-protective effects of bioactive fractions and in gredients in Sambucus williamsii HANCE", British Journa l of Nutrition, 106, pp.1802~ (2011)
- H. H. Xiao, "Phytochemicals and potential health effects of Sa mbucus williamsii Hance (Jiegumu)", Chinese Medicine, 11(36), pp.1~ (2016)
- J. Y. Shin, "Anti-inflammatory effect of red ginseng marc, Arte misia scoparia, Paeonia japonica and Angelical gigas ext ract mixture in LPS-stimulated RAW 264.7 cells", Biomed ical Reports, 17(1), pp.63~ (2022)
- M. Averbeck, "Differential regulation of hyaluronan metabolism in t he epidermal and dermal compartments of human skin by UVB irradiation", Journal of Investigative Dermatolog y, 127(3), pp.687~ (2007)
- M. Hara-Chikuma, "Roles of aquapori n-3 in the epidermis", Journal of Investigative Dermatology, 128(9), pp.2145~ (2008)
- M. S. Blois, "Antioxidant determinations by the use of a stable free radical", Nature, 181, pp.1199~ (1985)
- M. Yu, "Phytochemical and antioxidant analysis of medicinal a nd food plants towards bioactive food and pharmaceutical resources", Scientific Reports, 11(1), pp.10041~ (2021)
- 곽남, "흰우단버섯 추출물의 항산화와 항염 및 주름 개선 효능 평가", 한국화장품미용학회지, 12(1), pp.113~120 (2022), 한국화장품미용학회
- N. Vaou, "Interactions between Medical Plant-Derived Bioactive Compounds: Focus on Antimicrobial Combination Effects", Antibiotics (Basel), 11(8), pp.1014~ (2022)
- O. Folin, "On tyrosine and tryptophane determinations in proteins", Journal of Biological Chemistry, 73, pp.627~ (1927)
- R. Re, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radical Biology and Medicine, 26(9-10), pp.1231~ (1999)
- S. H. Park, "Loliolide presents antiapoptosis and antiscratching effects in human keratin ocytes", International Journal of Molecular Sciences, 20(3), pp.651~ (2019)
- S. Khoudali, "Total phenolic contents and antioxidant acitivity of methnalo extract from leves, flowers and stems of Warionia saharae L. from Morrocco", Journal of Biologically Active Producst from Nature, 8(5), pp.335~ (2018)
- S. Zhang, "Fighting against skin aging", Cell Transplant, 27(5), pp.729~ (2018)
- V. L. Singleton, "Anaylysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu", Methods in Enzymology, 299(14), pp.152~ (1999)
- W. S. Suh, "Iridoid glycosides from the twigs of Sam bucus williamsii var. coreana and Their biological activit ies", Journal of Natural Products, 80(9), pp.2502~ (2017)
- X. J. Yang, "Effect of phenolic acids isolated from Sambuc us Williamsii on proliferation and differentiation of rat osteoblastic UMR106 cells", Chinese Traditional and Herb a. Drugs, 36, pp.1604~ (2005)
- X. J. Yang, "Lignans from the stems of Sambucus william sii and their effects on osteoblastic UMR106 cells", Journa l of Asian Natural Products Research, 9, pp.583~ (2007)
- X. J. Yang, "Studies of trirepenoids isolated from Sambucus williamsii hance and their effects on UMR106 cell prolif eration and alkaline phosphatase activity", Journal of She nyang Pharmaceutical University, 22, pp.449~ (2005)
- Y. M. Muflihah, "Correlation study of antioxidant activity with phenolic and flavonoi d compounds in 12 indonesian indigenous herbs", Antiox idants (Basel), 10(10), pp.1530~ (2021)
- 소양강, "레몬그라스와 자소엽 추출물의 피부보습 및 피부장벽에 관한 연구", 대한화장품학회지, 45(3), pp.225~235 (2019), 사단법인 대한화장품학회[DOI]