Detection of Streptavidin-Biotin Complexes Using a Highly Sensitive AlGaN/GaN-Based Extended-Gate MISHEMT-Type Biosensor
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
In this paper, we propose an AlGaN/GaN-based extended-gate metal-insulator-semiconductor high electron mobility transistor (MISHEMT)-type biosensor for detecting streptavidin-biotin complexes. We measure the drain current of the fabricated sensor, which varies depending on the antibody-antigen reaction of streptavidin with biotin molecules. To confirm the immobilization of biotin polyethylene glycol (PEG) thiol, we analyze the Au surface of a GaN sample using X-ray photoelectron spectroscopy (XPS). The proposed biosensor shows higher sensitivity than Si-based extended-gate metal oxide semiconductor field effect transistor (MOSFET)-type biosensor. In addition, the proposed AlGaN/GaN-based extended-gate MISHEMT-type biosensor exhibits better long-term stability, compared to the conventional AlGaN/GaN-based MISHEMT-type biosensor.
Abstract
In this paper, we propose an AlGaN/GaN-based extended-gate metal-insulator-semiconductor high electron mobility transistor (MISHEMT)-type biosensor for detecting streptavidin-biotin complexes. We measure the drain current of the fabricated sensor, which varies depending on the antibody-antigen reaction of streptavidin with biotin molecules. To confirm the immobilization of biotin polyethylene glycol (PEG) thiol, we analyze the Au surface of a GaN sample using X-ray photoelectron spectroscopy (XPS). The proposed biosensor shows higher sensitivity than Si-based extended-gate metal oxide semiconductor field effect transistor (MOSFET)-type biosensor. In addition, the proposed AlGaN/GaN-based extended-gate MISHEMT-type biosensor exhibits better long-term stability, compared to the conventional AlGaN/GaN-based MISHEMT-type biosensor.
참고문헌 (15)
- K. E. Nelson, "Surface characterization of mixed self-assembled monolayers designed for streptavidin immobilization", Langmuir, 17, pp.2807~2816 (2001)
- B. K. Sohn, "A new pH-ISFET based dissolved oxygen sensor by employing electrolysis of oxygen", Sens. Actuator B-Chem, 34, pp.435~440 (1996)
- S. V. Dzyadevich, "Application of enzyme field-effect transistors for determination of glucose concentrations in blood serum", Biosens. Bioelectron, 14, pp.283~287 (1999)
- T. P. Chow, "Wide Bandgap Compound Semiconductors for Superior High-Voltage Unipolar Power Devices", IEEE Trans. Electron Devices, 41, pp.1481~1483 (1994)
- B. S. Kang, "Electrical detection of biomaterials using AlGaN", GaN high electron mobility transistors, 2008, pp.104, 031101~
- G. Steinhoff, "AlxGa1-xN-A New Material System for Biosensors", Adv. Funct. Mater, 13, pp.841~846 (2003)
- J. Choi, "Differential-Mode Biosensor Using Dual Extended-Gate Metal–Oxide–Semiconductor Field-Effect Transistors", Jpn. J. Appl. Phys., 51, pp.06FG05~ (2012)
- D. -S. Kim, "An extended gate FET-based biosensor integrated with a Si microfluidic channel for detection of protein complexes", Sens. Actuators B, 117, pp.488~494 (2006)
- J. -K. Shin, "Detection of DNA and Protein Molecules Using an FET-Type Biosensor with Gold as a Gate Metal", Electroanalysis, 16, pp.1912~1918 (2004)
- D. Y. Petrovykh, "Quantitative Analysis and Characterization of DNA Immobilized on Gold", J. Am. Chem. Soc, 125, pp.5219~5226 (2003)
- M. Mrksich, "Patterning self-assembled monolayers using microcontact printing : A new technology for biosensors?", Trends Biotechnol, 13, pp.228~235 (1995)
- Y. Wang, "AlGaN", GaN FET for DNA hybridization detection, 2011, pp.208, 1623~1625
- B. S. Kang, "Electrical detection of immobilized proteins with ungated AlGaN⁄ GaN high-electron-mobility Transistors", Appl. Phys. Lett, 87, pp.023508~ (2005)
- I. Kwon, "Extended-Gate Metal Oxide Semiconductor Field Effect Transistor-Based Biosensor for Detection of Deoxynivalenol", Jpn. J. Appl. Phys., 50, pp.06GL08~ (2011)
- B. S. Kang, "Prostate specific antigen detection using AlGaN", GaN high electron mobility transistors, 2006, pp.89, 122102~