허혈-재관류 실시 후 등장성 최대 근수축 운동에 따른 혈중 항산화 효소 활성에 미치는 영향
The Effect of Isotonic Contraction Exercise and Ischemia-Reperfusion on the Antioxidative Enzymes Activities
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본 연구의 목적은 허혈 -재관류 실시 후 등장성 최대 근 수축 운동에 따른 혈중 항산화 효소 활성에 미치는 영향을 알아보기 위해 유도선수 집단(n=7)과 일반 집단(n=7)을 대상으로 등장성 최대 근 수축 운동(18-20RM-dumbbell arm-curl)과 허혈- 재관류 실시 후 등장성 최대 근 수축 운동에 따른 안정 시, 운동 직후, 회복기 15분에 따른 혈중 항산화 효소 활성에 미치는 영향에 대해 알아보았다. 항산화 효소 활성도 변화를 비교하기 위해 이원 변량 분석을 실시하여 다음과 같은 결과를 얻었다. SOD 활성도의 변화는 유도선수 집단에서 등장성 최대 근 수축에 운동 후(p<.001)와 허혈-재관류 실시 후 등장성 최대 근 수축 운동에 따른 운동 직후와 회복기 15분에 활성도가 유의하게 높게 나타났다(p<.001). CAT 활성도의 변화는 등장성 최대 근 수축 운동에서는 집단 간 차이가 없었지만, 허혈-재관류 실시 후 등장성 최대 근 수축 운동에 따른 유도 선수 집단에서 운동 직후 유의하게 높게 나타났으며(p<.001), 회복기 15분에서는 유의하게 높게 나타났다(p<.05). 또한, GPX 활성도의 변화는 등장성 최대 근 수축 운동 시 회복기 15분에 유도 선수집단이 유의하게 높게 나타났다(p<.001). 결과적으로 등장성 최대 근 수축 운동 시 혈중 항산화 효소 활성은 유도선수 집단과 일반 집단에서 차이가 없었지만 허혈-재관류 실시 후 등장성 최대 근수축 운동에 따른 유도선수 집단이 일반 집단 보다 더 높게 나타났다. 이는 허혈-재관류에 따른 산화 스트레스가 증가하는 만큼 방어기전인 항산화 효소 활성 또한 빠르게 증가 되는 것을 알 수 있었으며 유도선수 집단이 일반 집단 보다 더 높은 SOD와 CAT가 나타난 것은 운동에 의한 산화적 내성이 일반 집단 보다 높기 떄문인 것으로 판단된다.
Abstract
The study was to examine that effective isotonic contraction exercise and Ischemia－Reperfusion after isotonic contraction exercise on SOD, CAT, GPX activity between elite athletics and normal people. The subjects in this study were divided into 2 groups according to their age (7 Judo athletics, mean age 21.60±1.05yr, 7 normal university 21.86±0.85yr) without any metabolic disease. The experiments were performed at the isotonic contraction maximum exercise (dumbbell arm curl 18-20RM for biceps) and ischemia－reperfusion test (blood flow was occluded by inflating a cuff about the upper arm to 230mmHg in sphygmomanometer) at the gym in Hanyang univ. In the main test, the blood samples were taken 3 times by 2 groups (before, immediately, recovery 15min), 8cc blood were collected each with heparinized1－ml syringes. The blood antioxidative enzyme level of subjects were analysed after blood sampling. The effects of isotonic contraction maximum exercise and ischemia-reperfusion after on isotonic contraction exercise on blood activities antioxidative enzyme (SOD, CAT, GPX) change between Judo athletics and normal people were as follow: The SOD result were shown ischemia- reperfusion after on isotonic contraction maximum exercise between 2group were decreased Judo athletics than normal group (p<.001). However, the ischemia- reperfusion test between group within SOD were increased in Juod athletics than normal group (p<.05). The CAT result were shown a ischemia- reperfusion test within CAT were increase in Judo athletics than in normal group (p<.001) The GPX result were shown a ischemia-reperfusion test within GPX were activities decrease in elite than in normal group. It is conclusively suggested that ischemia-reperfusion induced increase of oxidative stress increases the activity of antioxidant enzymes by maximal isotonic contraction, and these increase is higher in Judo athletics than in normal group.
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