최대하 운동의 강도에 따른 혈중 지질 과산화와 항산화 효소 활성의 변화
The effect of submaximal exercises with different intensities on the blood lipid peroxidation and antioxidant enzymes activities.
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최대하 운동의 강도에 따른 혈액 지질 과산화와 항산화 효소 활성의 변화. 운동과학, 제13권 제4호, 413-422, 2004. 본 연구의 목적은 일정 운동량을 소비하는 다른 강도의 최대하 운동에 따른 혈액의 지질 과산화과 항산화 효소 활성의 변화를 분석하는데 있다. 7명의 건강한 성인 남성들(연령: 25.6±1.5세, O2max: 56.0±2.7 ㎖/㎏/min)이 피험자로 선정되었고, 운동 강도를 4가지로 구분하여 이에 해당하는 운동 속도를 산출한 후 일정 운동량(400kcal)을 소비할 수 있도록 운동 시간을 개별적으로 정하여 트레드밀에서 달리기 운동을 실시하였다. 피험자별 운동 순서를 무선할당 및 배정하였고 1주일 간의 간격을 두어 실험하였다. 혈액은 안정 시, 운동 직후, 회복기 90분 째에 각각 10ml씩 전완주정맥에서 채혈하여 즉시 원심분리한 후 분석실로 운반하여 분석하였다. 지질 과산화 분석은 혈청 Malone dialdehyde(이하 MDA)를 분석하였고, 항산화 효소 활성은 적혈구에서의 glutathione peroxidase(GPx) and superoxide dismutase(SOD) 활성도를 분석하였다. 실험 결과, 혈청 MDA 농도는 운동 직후에 안정시보다 유의하게 증가하며(p<.05), 회복기 90분째 안정시 수준으로 감소하는 것으로 나타났다(p<.01). 적혈구 GPx 활성도는 운동에 따른 유의한 차이가 나타나지 않았으며, 적혈구 SOD 활성도는 전체적으로 운동 직후에 안정 시 보다 유의하게 감소하였고(p<.05), 회복기 90분 째에 약간 증가하지만, 안정 시 수준 보다 낮은 활성도를 보였다(p<.05). 따라서 400kcal 정도를 소비하는 최대하 운동은 운동 강도와 관계없이 일정 운동량에서 혈액내 산화 스트레스를 유발하지만 회복기 90분 이내에 안정 상태로 회복시키며, 적혈구 항산화 효소의 활성을 자극할 만큼 강하지는 않은 것으로 보인다.
Abstract
The effect of submaximal exercises with different intensities on the blood lipid peroxidation and antioxidant enzymes activities. Exercise Science, 13(4): 413-422, 2004. The purpose of this study is to examine the effects of various exercise intensities on the change of lipid peroxidation and antioxidant enzymes activities. 7 healthy young males(age : 25.6±1.5, O2max: 56.0±2.7 ml/kg/min) participated in four trials. At each trial., subjects ran on the treadmill at the respective intensities corresponding to 50%, 65%, 75% and 85% of O2max. They exercised for different duration so that energy expenditure(400kcal) was equal to all subjects. The order of the trials was randomized and separated by one week. Blood was obtained at rest, immediately after exercise, and at 90 min after exercise to measure the serum malondialdehyde(MDA) level, erythrocyte glutathione peroxidase(GPx) and superoxide dismutase(SOD) activities. serum MDA level was increased significantly immediately after exercise(p<.05) and recovered at 90 min after exercise(p<.01). Erythrocyte SOD activity decreased immediately after exercise in comparison to baseline(p<.05) and increased just a little at 90min after exercise but appeared lower activity than at rest(p<.05). Erythrocyte GPx activity did not significantly change throughout every time. Therefore, The results of this study suggest that submaximal exercise, which expend on 400kcal, induced the oxidative stress of the blood independent of exercise intensity but recovered into rest level within 90 minutes. And the exercise of this type does not stimulate activity of erythrocyte antioxidant enzymes
참고문헌 (28)
- "항산화제 복합제제 섭취기간에 따른 운동강도별 산화적 손상요소 MDA와 LDH의 차이", 41(2), pp.713~722 (2002)
- "ACSM' guidelines for exercise testing and prescription" (2000)
- "Elevated serum antioxidant capacity and plasma MDA concentration in response to a simulated half-marathon run", pp.1603~1607 (1998)
- "Effects of a training taper on tissue damage indices serum antioxidant capacity half-marathon running performance" (2000)
- "Blood glutathione status following distance running" (1997)
- "Exercise free radical generation and aging", 9, pp.1218~ (1997)
- "Effects of graded treadmill exercise on the activity of blood antioxidant enzymes lipid peroxides and nonenzymatic anti- oxidants in long-distance skiers", pp.217~226 (1994)
- "Exercise and oxidative stress role of the cellular antioxidant systems", pp.135~166 (1995)
- "Alteration of antioxidant enzymes with aging in rat skeletal muscle and liver" (1990)
- "Antioxidants and oxidative stress in exercise" (1999)
- "Free radicals and exercise Effects of nutritional antioxidant supplementation", pp.375~397 (1995)
- "Effects of an antioxidant vitamin mixture on lipid peroxidation at rest and postexercise", pp.965~969 (1993)
- "Ubiquinone supplementation and exercise capacity in trained young and older men", pp.95~100 (1995)
- "The effect of exercise intensity on lipid peroxidation", pp.1036~1038 (1997)
- "Glutathione depletion in rested and exercised mice biochemical consequence and adaptation", pp.941~949 (1995)
- "Alteration of glutathione and antioxidant status whith exercise in unfed and refed rats"
- "Oxidative stress and antioxidant in exercise", pp.829~838 (2001)
- "Blood free radical antioxidant enzymes and lipid peroxides following long-distance and lactacidemic performances in highly trained aerobic and sprint athletes" (1997)
- "Protective effect of vitamin E on exercise-induced oxidative damage in young and older adults", pp.~998 (1993), Fiatarone
- "Strenuous endurance training in humans reduces oxidative stress following exhausting exercise", pp.1 1~2 6 (2001)
- "DNA damage after exhaustive treadmill running in trained and untrained men" (1996)
- "Alteration of superoxide dismutase iso-enzyme activity and mRNA expression with aging in rat skeletal muscle", pp.65~76 (1996)
- "Plasma glutathione peroxidase in healthy young adults influence of gender and physical activity", pp.345~351 (2003)
- "Regulation of erythrocyte antioxidant enzyme activities in athletes during competition and short-term recovery", gime, pp.782~787 (1999)
- "Lack of antioxidant adaptation to short-term aerobic training in human muscle" (1996)
- "Oxidative stress , and antioxidant supplementation", pp.41~54 (2003)
- "Measurement of serum lipid peroxidation during exercise using three different methods:diene conjugation thiobarbituric acid reactive material and fluorescent chromolipids", pp.63~69 (1995)
- "Exercise-induced oxidative stress affects erythrocytes in sedentary rats but not exercise-trained rats"