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트레드밀 운동과 밝은 빛이 흰쥐 뇌의 세로토닌 발현에 미치는 영향

Effects of Treadmill Exercise and Bright Lighton Serotonin Expression in Rat Brain

송광섭|권선옥|권성진
국민체육진흥공단 한국스포츠과학원 (Korea Institute of Sport Science)|2014.06|체육과학연구 (Korean Journal of Sport Science)|제25권 제2호|pp. 193-201|DOI 10.24985/kjss.2014.25.2.193

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참고문헌

키워드

운동ExerciseBrain뇌Serotonin세로토닌밝은 빛Bright Light
주제분류체육

초록

이 연구는 트레드밀 운동과 밝은 빛이 흰쥐 뇌의 세로토닌 발현에 미치는 영향을 조사하는데 그 목적이 있다. 수컷 흰쥐는 임의로 아홉 마리씩 네 집단(통제집단, 운동집단, 빛집단, 운동+빛집단)으로 분류하였다. 운동집단은 일주일에 5일, 하루에 30분씩 운동하였고, 빛집단은 일주일에 5일 하루에 30분씩 10,000 lux의 빛을 쬐었으며 운동+빛집단은 운동과 빛을 위 두 방식대로 복합처치 되었다. 실험기간은 4주간이며 통제집단은 아무런 실험처치 없이 사육되었다. 우리는 통제집단에 비해 나머지 집단에서 회봉선핵과 중뇌에서 세로토닌의 발현이 유의미하게 증가된 것을 알았다. 흥미로운 것은 세로토닌의 발현이 면역조직화학법에서 운동집단과 빛집단에 비해 운동+빛집단에서 유의미하게 증가된 것이고, 면역얼룩법에서도 유의미한 증가는 아니지만 증가의 양상을 보였다는 것이다. 결과적으로 트레드밀 운동과 밝은 빛은 뇌의 세로토닌 발현에 긍정적인 효과가 있음을 알 수 있었다. 그러므로 우리는 성장기에 밝은 빛을 쬐면서 운동을 하는 것은 뇌 활동에 도움이 될 것이라 제안한다.

Abstract

The main purpose of this study was to investigate the effects of treadmill exercise and bright light exposure on serotonin expression in rat brain. Male Sprague-Dawley (SD) rats were randomly assigned into four groups (n=9 in each group), specifically, control group (CG), exercise group (EG), light group (LG), and exercise+light group (ELG). Rats in EG were subjected to treadmill exercise (5 days/week, 30 min/day), LG rats exposed (5 days/week, 30 min/day, 10,000 Lux), ELG rats subjected to treadmill exercise in combination with exposure, and CG rats remained sedentary over a four-week period. We observed a significant increase in serotonin expression in the raphe obscurus nucleu and the midbrain of rats in EG, LG, and ELG, compared to CG. Interestingly, serotonin expression was significantly increased in ELG, compared to EG and LG in the raphe obscurus nucleu via immunohistochemistry. In the western blot, it showed a increased pattern in ELG, compared to EG and LG. The overall results showed that treadmill exercise and/or bright light had positive effects on serotonin expression in the brain. Therefore, we suggest that moderate exercise or exposure to bright light during a growth child may be beneficial in brain action.

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