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Stress-Induced Depression Is Alleviated by Aerobic Exercise Through Up-Regulation of 5-Hydroxytryptamine 1A Receptors in Rats

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Purpose: Stress is associated with depression, which induces many psychiatric disorders. Serotonin, also known as 5-hydroxytryptamine(5-HT), acts as a biochemical messenger and regulator in the brain. It also mediates several important physiologicalfunctions. Depression is closely associated with an overactive bladder. In the present study, we investigated the effect oftreadmill exercise on stress-induced depression while focusing on the expression of 5-HT 1A (5-HT1A) receptors in the dorsalraphe. Methods: Stress was induced by applying a 0.2-mA electric foot shock to rats. Each set of electric foot shocks comprised a6-second shock duration that was repeated 10 times with a 30-second interval. Three sets of electric foot shocks were appliedeach day for 7 days. For the confirmation of depressive state, a forced swimming test was performed. To visualize the expressionof 5-HT and tryptophan hydroxylase (TPH), immunohistochemistry for 5-HT and TPH in the dorsal raphe was performed. Expression of 5-HT1A receptors was determined by western blot analysis. Results: A depressive state was induced by stress, and treadmill exercise alleviated the depression symptoms in the stress-inducedrats. Expressions of 5-HT, TPH, and HT 1A in the dorsal raphe were reduced by the induction of stress. Treadmill exerciseincreased 5-HT, TPH, and HT 1A expressions in the stress-induced rats. Conclusions: Treadmill exercise enhanced 5-HT synthesis through the up-regulation of 5-HT1A receptors, and improved thestress-induced depression. In the present study, treadmill exercise improved depression symptoms by enhancing 5-HT1A receptorexpression. The present results suggest that treadmill exercise might be helpful for the alleviation of overactive bladderand improve sexual function.

Abstract

Purpose: Stress is associated with depression, which induces many psychiatric disorders. Serotonin, also known as 5-hydroxytryptamine(5-HT), acts as a biochemical messenger and regulator in the brain. It also mediates several important physiologicalfunctions. Depression is closely associated with an overactive bladder. In the present study, we investigated the effect oftreadmill exercise on stress-induced depression while focusing on the expression of 5-HT 1A (5-HT1A) receptors in the dorsalraphe. Methods: Stress was induced by applying a 0.2-mA electric foot shock to rats. Each set of electric foot shocks comprised a6-second shock duration that was repeated 10 times with a 30-second interval. Three sets of electric foot shocks were appliedeach day for 7 days. For the confirmation of depressive state, a forced swimming test was performed. To visualize the expressionof 5-HT and tryptophan hydroxylase (TPH), immunohistochemistry for 5-HT and TPH in the dorsal raphe was performed. Expression of 5-HT1A receptors was determined by western blot analysis. Results: A depressive state was induced by stress, and treadmill exercise alleviated the depression symptoms in the stress-inducedrats. Expressions of 5-HT, TPH, and HT 1A in the dorsal raphe were reduced by the induction of stress. Treadmill exerciseincreased 5-HT, TPH, and HT 1A expressions in the stress-induced rats. Conclusions: Treadmill exercise enhanced 5-HT synthesis through the up-regulation of 5-HT1A receptors, and improved thestress-induced depression. In the present study, treadmill exercise improved depression symptoms by enhancing 5-HT1A receptorexpression. The present results suggest that treadmill exercise might be helpful for the alleviation of overactive bladderand improve sexual function.

참고문헌 (31)

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