Optimization of Aqueous Methanol Extraction Condition of Total Polyphenol from Spent Lycium chinense Miller to Develop Feed Additives for Pig
0
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
This study was conducted to develop a functional feed additive for pig with spent Lycium chinense Mill fruit. We investigated the optimum conditions for the extraction of polyphenol from spent Lycium chinense Mill using methanol. Methanol concentration as a solvent for extraction, extraction time and the volume of solvent per a gram of solid (ground spent Lyceum chinense Mill) were selected as parameters. Three levels of parameters were configured according to Box Behnken experiment design, a fractional factorial design, and total 15 trials were employed. Total polyphenol concentration from each trial was used as response from experiment system and effects of parameters on total polyphenol extraction efficiency were determined using response surface model. As a result, all terms in analysis of variance, regression (p=0.001), linear (p=0.002), square (p=0.017) and interaction (p=0.047) was significant and adjusted determination coefficient (R2) was 94.7%. Total polyphenol extraction efficiency was elevated along increased methanol content and decreased solvent to solid ratio. However extraction time did not affect the efficiency. This study provides a primary information for the optimum extraction conditions to maximize total polyphenol recovery from spent Lycium chinens Mill fruit and this result could be applied to re-use of argo-industrial by-products and to develop of functional feed additives in organic farming.
Abstract
This study was conducted to develop a functional feed additive for pig with spent Lycium chinense Mill fruit. We investigated the optimum conditions for the extraction of polyphenol from spent Lycium chinense Mill using methanol. Methanol concentration as a solvent for extraction, extraction time and the volume of solvent per a gram of solid (ground spent Lyceum chinense Mill) were selected as parameters. Three levels of parameters were configured according to Box Behnken experiment design, a fractional factorial design, and total 15 trials were employed. Total polyphenol concentration from each trial was used as response from experiment system and effects of parameters on total polyphenol extraction efficiency were determined using response surface model. As a result, all terms in analysis of variance, regression (p=0.001), linear (p=0.002), square (p=0.017) and interaction (p=0.047) was significant and adjusted determination coefficient (R2) was 94.7%. Total polyphenol extraction efficiency was elevated along increased methanol content and decreased solvent to solid ratio. However extraction time did not affect the efficiency. This study provides a primary information for the optimum extraction conditions to maximize total polyphenol recovery from spent Lycium chinens Mill fruit and this result could be applied to re-use of argo-industrial by-products and to develop of functional feed additives in organic farming.
참고문헌 (15)
- Box, G. E. P., "Some new three level designs for the study of quantitative variables", Technometrics, 2, pp.455~475 (1960)
- 장우경, "가축용 생균제 Lactobacillus plantarum을 이용한 양파즙 발효의 균체성장과 항산화 활성", 생명과학회지, 20(11), pp.1729~1737 (2010), 한국생명과학회
- Chirinos, R., "Optimization of extraction conditions of antioxidant phenolic compounds from mashua (Tropaeolum tuberosum Ruíz & Pavón) tubers", Sep. Purif. Technol, 55, pp.217~225 (2007)
- 조상범, "Effects of Plant Oils and Minerals for the Inhibition of Lipase Activity of Staphylococcus aureus Isolated from Fermented Pork Meat", 한국축산식품학회지, 30(5), pp.764~772 (2010), 한국축산식품학회
- Cohran, W, "Experimental design" (2002), Wiley
- Juan, M.-Y., "Enhancement of antioxidant activity, total phenolic and flavonoid content of black soybeans by solid state fermentation with Bacillus subtilis BCRC 14715", Food Microbiol, 27, pp.586~591 (2009)
- Jung, S., "Effect of dietary mixture of gallic acid and linoleic acid on antioxidative potential and quality of breast meat from broilers", MEAT SCIENCE, 86, pp.520~526 (2010), ELSEVIER SCI LTD
- Kaushik, R., "Statistical optimization of medium components and growth conditions by response surface methodology to enhance lipase production by Aspergillus carneus", J. Mol. Catal. B: Enzym, 40, pp.121~126 (2006)
- Kim, M., "The antioxidative effects of the water-soluble extracts of plants used as tea materials", Korean J. Food. Sci. Technol, 33, pp.12~18 (2001)
- Lonni, A. A. S. G., "Statistical mixture design selective extraction of compounds with antioxidant activity and total polyphenol content from Trichilia catigua", Anal. Chim. Acta, 719, pp.57~60 (2012)
- Mussatto, S. I., "Extraction of antioxidant phenolic compounds from spent coffee grounds", Sep. Purif. Technol, 83, pp.173~179 (2011)
- Oliveira, R. A., "Effects of feeding polyphenols from pomegranate extract on health, growth, nutrient digestion, and immunocompetence of calves", J. Dairy Sci, 93, pp.4280~4291 (2010)
- Rice-Evans, C. A., "Antioxidant properties of phenolic compound", Trends in Plant Science, 2, pp.152~159 (1997)
- Soni, P., "Response surface optimization of the critical medium components for carbonyl reductase production by Candida viswanathii MTCC 5158", Bioresour. Technol, 98, pp.829~833 (2007)
- Zhang, R., "Cytoprotective effect of the fruits of Lycium chinense Miller against oxidative stress-induced hepatotoxicity", J. Ethnopharmacol, 130, pp.99~306 (2010)