녹나무 잎의 반응표면분석법을 이용한 환류냉각 추출조건 최적화 및 생리활성
Optimizing the Reflux Extraction Conditions using Response Surface Methodology and Physiological Activity of Cinnamomum camphora Leaves
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Experiments were conducted to increase the efficiency of extracting bioactive compoundsfrom Cinnamomum camphora leaves which are industrially used in various fields. Responsesurface methodology (RSM) maximized the total polyphenol content in extracting C. camphoraleaves. The experimental design was conducted for three factors: extraction temperature, ethanolconcentration, and solid-liquid ratio. The optimum conditions for the total polyphenol content ofC. camphora leave extract were found to be an extraction temperature of 80°C, an ethanolconcentration of 55%, and a solid ratio of 100; the predicted value was confirmed to be 57.67 ±0.66 mg GAE/g. As a result of the experiment, the total polyphenol content of the optimizedextract of C. camphora leaves was 57.29 ± 0.41 mg GAE/g, and the total flavonoid content was10.57 ± 0.20 mg QE/g. The antioxidant activity was also measured at 25.98 ± 0.67 μg/mL forDPPH RC50, higher than the control group BHT (122.69 ± 10.55 μg/mL). TEAC was 239.39 ±11.28 μM TE/g, and FRAP was 2,049.62 ± 32.45 mM FE/g. Moreover, the inhibitory effect oftyrosinase and Staphylococcus epidermidis was confirmed. The results of this study suggest theoptimal conditions for maximum extraction of total polyphenol content from C. camphora leaves,and it is expected to be essential data for industrial use through the physiological activity ofoptimized extracts.
Abstract
Experiments were conducted to increase the efficiency of extracting bioactive compoundsfrom Cinnamomum camphora leaves which are industrially used in various fields. Responsesurface methodology (RSM) maximized the total polyphenol content in extracting C. camphoraleaves. The experimental design was conducted for three factors: extraction temperature, ethanolconcentration, and solid-liquid ratio. The optimum conditions for the total polyphenol content ofC. camphora leave extract were found to be an extraction temperature of 80°C, an ethanolconcentration of 55%, and a solid ratio of 100; the predicted value was confirmed to be 57.67 ±0.66 mg GAE/g. As a result of the experiment, the total polyphenol content of the optimizedextract of C. camphora leaves was 57.29 ± 0.41 mg GAE/g, and the total flavonoid content was10.57 ± 0.20 mg QE/g. The antioxidant activity was also measured at 25.98 ± 0.67 μg/mL forDPPH RC50, higher than the control group BHT (122.69 ± 10.55 μg/mL). TEAC was 239.39 ±11.28 μM TE/g, and FRAP was 2,049.62 ± 32.45 mM FE/g. Moreover, the inhibitory effect oftyrosinase and Staphylococcus epidermidis was confirmed. The results of this study suggest theoptimal conditions for maximum extraction of total polyphenol content from C. camphora leaves,and it is expected to be essential data for industrial use through the physiological activity ofoptimized extracts.
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