반응표면 분석법을 이용한 병아리콩 항산화활성 추출의 최적화 조건
Establishment of Optimum Extraction Conditions for Antioxidant Activity of Chickpea by Response Surface Methodology
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This study was done to determine the optimum conditions from various solvent type(ethanol, methanol), solvent concentration(60, 70, 80%, v/v), extraction time(30, 80, 130 min.) and extraction temperature(10, 15, 20℃) in order to increase the antioxidant activity of the chickpea. DPPH radical scavenging activity was measured by using response surface methodology(RSM) to establish the optimal conditions. In the treatment of ethanol extractions, 80% of concentration for 130 min. at 20℃ showed maximum level of DPPH radical scavenging activity of 76.13%(R2=0.9435). However DPPH radical scavenging activity of methanol extraction was 73.28%(R2=0.9427) from 70% concentration at 20℃ for 130 min. The results indicated that ethanol extracts showed a higher antioxidant activity than that from methanol extracts. The ethanol extraction conditions of response surface analysis(RSA) were affected more by extraction time or concentration than solvent temperature(p<0.05). The methanol extraction conditions of RSA showed same resulted with the condition of ethanol extracts(p<0.001). Based on the RSM, the predicted best conditions of ethanol extraction were 72.88% of solvent concentration at 15.72℃ for 124.72 min. And also predicted best condition of methanol extraction were 70.16% of solvent concentration at 18.03℃ for 117.12 min.
Abstract
This study was done to determine the optimum conditions from various solvent type(ethanol, methanol), solvent concentration(60, 70, 80%, v/v), extraction time(30, 80, 130 min.) and extraction temperature(10, 15, 20℃) in order to increase the antioxidant activity of the chickpea. DPPH radical scavenging activity was measured by using response surface methodology(RSM) to establish the optimal conditions. In the treatment of ethanol extractions, 80% of concentration for 130 min. at 20℃ showed maximum level of DPPH radical scavenging activity of 76.13%(R2=0.9435). However DPPH radical scavenging activity of methanol extraction was 73.28%(R2=0.9427) from 70% concentration at 20℃ for 130 min. The results indicated that ethanol extracts showed a higher antioxidant activity than that from methanol extracts. The ethanol extraction conditions of response surface analysis(RSA) were affected more by extraction time or concentration than solvent temperature(p<0.05). The methanol extraction conditions of RSA showed same resulted with the condition of ethanol extracts(p<0.001). Based on the RSM, the predicted best conditions of ethanol extraction were 72.88% of solvent concentration at 15.72℃ for 124.72 min. And also predicted best condition of methanol extraction were 70.16% of solvent concentration at 18.03℃ for 117.12 min.
참고문헌 (28)
- 권유리, "추출용매에 따른 모링가(Moringa oleifera Lam.) 잎의 항산화 및 생리활성 효과", 한국식품저장유통학회지, 21(6), pp.831~837 (2014), 한국식품저장유통학회
- 손종연, "석류씨 추출물의 항산화 및 항균활성", 한국식품조리과학회지, 21(2), pp.171~179 (2005), 한국식품조리과학회
- 남상명, "산사 추출물의 항산화 및 항당뇨 활성", 동아시아식생활학회지, 25(2), pp.270~277 (2015), 동아시아식생활학회[DOI]
- 김지영, "에탄올-물과 메탄올-물 혼합 용매를 이용한 대두 추출물의 항산화 효과", 한국조리과학회지, 12(4), pp.493~498 (1996)
- 김수정, "감초의 에탄올 추출물의 항산화 활성 및 안정성 조사", 한국식품과학회지, 38(4), pp.584~588 (2006), 한국식품과학회
- 김순임, "추출 조건에 따른 오미자 추출물의 항산화 및 혈당 강하에 관한 연구", 한국식품영양학회지, 22(1), pp.41~47 (2009), 한국식품영양학회
- 김선경, "추출 조건을 달리한 감잎과 녹차의 물 및 에탄올 추출물이 노령쥐의 지방대사와 항산화능에 미치는 영향", 한국영양학회지, 34(5), pp.499~512 (2001)
- 박건희, "유산균 발효 병아리콩 두유를 첨가한 떡의 항산화 항균활성에 대한 연구", International Symposium and Annual Meeting, pp.337~338 (2014), 한국식품영양과학회
- 박혜미, "추출용매에 따른 아로니아 추출물의 생리 활성", 한국식품저장유통학회지, 21(5), pp.718~726 (2014), 한국식품저장유통학회
- 손종연, "밀, 쌀, 현미 및 보리로 제조한 시판 국수류의 항산화 특성", 한국외식산업학회지, 10(2), pp.95~103 (2014), 한국외식산업학회
- 이미희, "추출 용매에 따른 산딸기 추출물의 항산화 및 항균 활성", 한국식품영양학회지, 28(5), pp.774~781 (2015), 한국식품영양학회
- 이성구, "추출용매에 따른 진피 추출물의 항산화 활성", 한국식품영양학회지, 28(3), pp.458~464 (2015), 한국식품영양학회
- 이승언, "부추의 ascorbic acid 함량 및 항산화 활성", 한국외식산업학회지, 7(2), pp.87~99 (2011), 한국외식산업학회
- 이영자, "녹차, 우롱차 및 홍차의 용매별 추출물의 카테킨류 함량 및 항산화효과에 관한 연구", 한국식품위생안전성학회, 13(4), pp.370~376 (1998)
- 이윤서, "자연을 닮은 밥상", pp.200~202 (2013), 위즈덤하우스
- 이진선, "병아리 콩 첨가한 양갱의 품질특성", 석사, pp.2015
- 이호선, "병아리콩(Cicer arietinum L. cv. Amethyst) 근류내의 플라스티드 포스포프룩토오스 키나아제의 분리 및 특성", 한국농화학회지, 41(5), pp.355~362 (1998)
- 정중근, "한국산 고구마의 기관별, 부위별, 품종별, 조리방법에 따른 총 폴리페놀 함량과 항산화 활성에 관한 연구", 한국외식산업학회지, 9(2), pp.7~19 (2013), 한국외식산업학회
- 한기영, "반응표면 분석법을 이용한 치아씨 항산화 활성 추출의 최적화 조건", 한국식품영양학회지, 29(2), pp.228~236 (2016), 한국식품영양학회
- Akhtar, M. S., "Effects of AM Fungi on the plant growth and root-rot disease of chickpea", American-Eurasian J. Agric. & Environ. Sci, 8(5), pp.544~549 (2010)
- Blois, M. S., "Antioxidant determinations by the use of a stable free radical", Nature, 181(4617), pp.1199~1200 (1958)
- 정현정, "Studies for Physicochemical and In Vitro Digestibility Characteristics of Flour and Starch from Chickpea (Cicer arietinum L.)", Preventive Nutrition and Food Science, 16(4), pp.339~347 (2011), 한국식품영양과학회
- Handa, C. L., "Multi-response optimisation of the extraction solvent system for phenolics and antioxidant activities from fermented soy flour using a simplex-centroid design", Food Chem, 197, pp.175~184 (2016)
- Mohd, S. A., "Effects of AM Fungi on the plant growth and root-rot disease of chickpea", American-Eurasian J. Agric. & Environ. Sci, 8(5), pp.544~549 (2010)
- Mokrani, A., "Effect of solvent, time and temperature on the extraction of phenolic compounds and antioxidant capacity of peach(Prunus persica L.) fruit", Separation Purification Technol, 162, pp.68~76 (2016)
- Nalaini, D. S., "Heat and mass transfer during cooking of chickpea-measurements and computational simulation", Master, pp.2005
- Pittaway, J. K., "Chickpeas and human health, the effect of chickpea consumption on some physiological and metabolic parameters", Master, pp.2006
- Liu, Y., "Acute effects of navy bean powder, lentil powder and chickpea powder on post prandial glycaemic response and subjective appetite in healthy young men", Master, pp.2012