상백피 추출물의 나노입자화와 에탄올 추출물의 항산화 효과 비교
Comparison of Antioxidant Effects of the Nano-encapsulated and Ethanol Extracts from Mulberry (M. alba L. ) Root Bark
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Nanoencapsulation is the coating of various substances within another material at sizes on the nanoscales and it is prospected in all kinds of industries especially cosmetics, pharmaceutics, and food manufacturing. This study was performed to investigate the antioxidant activities as comparing ethanol extracts (ME) and nano-encapsulation of Mulberry Root Bark Extracts by lecithin and cholesterol (NM). DPPH radical scavenging activity of both extracts are almost identical for the 250 ug/ml samples. Also, the effects on nano-encapsulated extracts (NM) and ethanol extracts (ME) on the generation (induction) of Reactive Oxygen Species (ROS)by adding LPS to induce oxygen stress into the cell was that the both extracts in adding sample (500 ㎍/㎖)or (1000 ㎍/㎖) showed similar to oxidative stresses inhibition compared to 50% of control. ln addition, cell viability of the nano-encapsulated extracts (NM) was measured on RAW 264.7 cells and showed higher than that of ethanol extract (ME) in each concentration. The size of the nano-encapsulated extracts (NM) was an average diameter of range of 200-400 nm so they can effectively penetrate cell more than ethanol extract (ME)as nano-encapsulated extracts (NM) have great mobility and affinity for cell because of their small size. it was observed by confocal laser scanning microscope.
Abstract
Nanoencapsulation is the coating of various substances within another material at sizes on the nanoscales and it is prospected in all kinds of industries especially cosmetics, pharmaceutics, and food manufacturing. This study was performed to investigate the antioxidant activities as comparing ethanol extracts (ME) and nano-encapsulation of Mulberry Root Bark Extracts by lecithin and cholesterol (NM). DPPH radical scavenging activity of both extracts are almost identical for the 250 ug/ml samples. Also, the effects on nano-encapsulated extracts (NM) and ethanol extracts (ME) on the generation (induction) of Reactive Oxygen Species (ROS)by adding LPS to induce oxygen stress into the cell was that the both extracts in adding sample (500 ㎍/㎖)or (1000 ㎍/㎖) showed similar to oxidative stresses inhibition compared to 50% of control. ln addition, cell viability of the nano-encapsulated extracts (NM) was measured on RAW 264.7 cells and showed higher than that of ethanol extract (ME) in each concentration. The size of the nano-encapsulated extracts (NM) was an average diameter of range of 200-400 nm so they can effectively penetrate cell more than ethanol extract (ME)as nano-encapsulated extracts (NM) have great mobility and affinity for cell because of their small size. it was observed by confocal laser scanning microscope.
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