모링가:두충 2:1 (g:g) 복합 조성물의 in vitro 항산화 및 항염 효능 연구
In vitro Anti-oxidant and Anti-inflammatory Effects of Moringa Folium and Eucommiae Cortex 2:1 (g/g) Mixed Formula
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The purpose of study is to evaluate in vitro anti-oxidant and anti-inflammatory effects of Moringa Folium and Eucommiae Cortex 2:1 (g/g) mixtures (MEMix). HaCaT and human normal dermal fibroblast were treated with 0.01-1 mg/mL of MEMix to monitor cytotoxicity. Radical scavenging activities of MEMix were examined by DPPH assay. To explore anti-inflammatory effect, Raw 264.7 cells were pretreated with MEMix for 1h and subsequently exposed to LPS for 18h. NO release and cytotoxicity of Raw 264.7 cells were measured by adding Griess and MTT reagents, respectively. TNF-α, IL-1β, IL-6, and PGE2 productions were examined by ELISA. Immunoblot analysis was conducted to examine COX-2 expression in MEMix pretreated Raw 264.7 cells. Up to 1 mg/mL concentration, treatment of MEMix for 24 h did not affect normal dermal fibroblast viability and significantly reduced cell viability of HaCaT cells with no concentration dependency. MEMix increased DPPH radical scavenging activity with concentration dependency. Radical scavenging activities by 1 mg/mL of MEMix was comparable with 30 μM of trolox. Pretreatment of MEMix did not change the reduction of Raw 264.7 cell viability. Exposure of LPS in Raw 264.7 cells significantly increased NO, TNF-α, IL-1β, IL-6, and PGE2 productions, and MEMix pretreatment attenuated these productions by LPS concentration dependently. However, pretreatment with MEMix did not change COX-2 expression by LPS in Raw 264.7 cells. MEMix showed in vitro anti-oxidant and anti-inflammatory activities. MEMix would be useful candidate agent against inflammation.
Abstract
The purpose of study is to evaluate in vitro anti-oxidant and anti-inflammatory effects of Moringa Folium and Eucommiae Cortex 2:1 (g/g) mixtures (MEMix). HaCaT and human normal dermal fibroblast were treated with 0.01-1 mg/mL of MEMix to monitor cytotoxicity. Radical scavenging activities of MEMix were examined by DPPH assay. To explore anti-inflammatory effect, Raw 264.7 cells were pretreated with MEMix for 1h and subsequently exposed to LPS for 18h. NO release and cytotoxicity of Raw 264.7 cells were measured by adding Griess and MTT reagents, respectively. TNF-α, IL-1β, IL-6, and PGE2 productions were examined by ELISA. Immunoblot analysis was conducted to examine COX-2 expression in MEMix pretreated Raw 264.7 cells. Up to 1 mg/mL concentration, treatment of MEMix for 24 h did not affect normal dermal fibroblast viability and significantly reduced cell viability of HaCaT cells with no concentration dependency. MEMix increased DPPH radical scavenging activity with concentration dependency. Radical scavenging activities by 1 mg/mL of MEMix was comparable with 30 μM of trolox. Pretreatment of MEMix did not change the reduction of Raw 264.7 cell viability. Exposure of LPS in Raw 264.7 cells significantly increased NO, TNF-α, IL-1β, IL-6, and PGE2 productions, and MEMix pretreatment attenuated these productions by LPS concentration dependently. However, pretreatment with MEMix did not change COX-2 expression by LPS in Raw 264.7 cells. MEMix showed in vitro anti-oxidant and anti-inflammatory activities. MEMix would be useful candidate agent against inflammation.
참고문헌 (40)
- Habashy RR, "Anti-inflammatory effects of jojoba liquid wax in experimental models", Pharmacol Res, 51(2), pp.95~105 (2005)
- Williams RC, "Periodontal disease", N Engl J Med, 322(6), pp.373~382 (1990)
- Williams RC, "Understanding the pathogenesis of periodontitis : a century of discovery", J Int Acad Periodontol, 2(3), pp.59~63 (2000)
- Sallay K, "Alveolar bone destruction in the immunosuppressed rat", J Periodontal Res, 17(3), pp.263~274 (1982)
- Samejima Y, "Effect of infection with Eikenella corrodens on the progression of ligature-induced periodontitis in rats", J Periodontal Res, 25(5), pp.308~315 (1990)
- Botelho MA, "Lippia sidoides and Myracrodruon urundeuva gel prevents alveolar bone resorption in experimental periodontitis in rats", J Ethnopharmacol, 113(3), pp.471~478 (2007)
- Reddy MS, "Periodontal host modulation with antiproteinase, anti-inflammatory, and bone-sparing agents : a systematic review", Ann Periodontol, 8(1), pp.12~37 (2003)
- Bhatavadekar NB, "New directions in host modulation for the management of periodontal disease", J Clin Periodontol, 36(2), pp.124~126 (2009)
- Kim S, "The current state and effect of the drugs for the treatment of periodontal disease in market", pp.2016
- 민대진, "녹차추출물의 잇몸 질환 원인균에 대한 항염증 효능 연구", 대한화장품학회지, 37(1), pp.67~73 (2011), 사단법인 대한화장품학회
- 이동재, "치주 질환 예방 및 치료용 소재로서 수종의 생약성분 추출물에 관한 항균, 항염, 항산화 효능 연구", 치위생과학회지, 10(1), pp.25~29 (2010), 한국치위생과학회
- Park YH, "Ameliorating Effects of Curcumin on the Ligation-induced Experimental Periodontitis and Alveolar Bone Loss in Rats", pp.2019
- Park, J.H., "Anti-Periodontitis Effects of Magnolia obovata Thunberg. Cortex with Zea Mays L. Extract on the Ligature-Induced Periodontitis Rat", pp.2016
- Cheenpracha S, "Potential anti-inflammatory phenolic glycosides from the medicinal plant Moringa oleifera fruits", Bioorg Med Chem, 18(17), pp.6598~6602 (2010)
- Kim DS, "Phytochemical analysis, antioxidant and antiobesity effects of Moringa oleifera leaves extracts", pp.2019
- 최영주, "모링가 잎 추출물의 항당뇨, 알코올 대사 및 간 보호 활성", 한국식품영양과학회지, 45(6), pp.819~827 (2016), 한국식품영양과학회
- Ghasi S, "Hypocholesterolemic effects of crude extract of leaf of Moringa oleifera Lam in high-fat diet fed wistar rats", J Ethnopharmacol, 69(1), pp.21~25 (2000)
- Okuda T, "Coagulation mechanism of salt solution-extracted active component in Moringa oleifera seeds", Water Res, 35(3), pp.830~834 (2001)
- Zheng, J, "Protective effects of different extracts of Eucommia ulmoides Oliv. against thioacetamide-induced hepatotoxicity in mice", Indian J Exp Biol, 50(12), pp.875~882 (2012)
- Hsieh CL, "Antioxidant actions of Du-zhong (Eucommia ulmoides OLIV.) toward oxidative damage in biomolecules", Life Sci, 66(15), pp.1387~1400 (2000)
- Bong Hyun Kim, "Elucidation of Anti-inflammatory Potencies of Eucommia ulmoides Bark and Plantago asiatica Seeds", Journal of Medicinal Food, 12(4), pp.764~769 (2009), 한국식품영양과학회
- Kwon SH, "Neuroprotective effects of Eucommia ulmoides Oliv. bark on amyloid beta(25-35)-induced learning and memory impairments in mice", Neurosci Lett, 487(1), pp.123~127 (2011)
- Lin J, "Eucommia ulmoides Oliv. antagonizes H2O2-induced rat osteoblastic MC3T3-E1 apoptosis by inhibiting expressions of caspases 3, 6, 7, and 9", J Zhejiang Univ Sci B, 12(1), pp.47~54 (2011)
- HLSCIENCE Co., LTD, "Method for improving periodontitis and alveolar bone loss of complex extracts of Moringa leaf and Eucommia Bark by anti-bacterial, antioxidant, anti-inflammatory, antioxidant effects and inhibition of alveolar bone loss", 10-2380781-0000, ~ (2022)
- Kim DO, "Quantification of polyphenolics and their antioxidant capacity in fresh plums", J Agric Food Chem, 51(22), pp.6509~6515 (2003)
- Luis Anel-López, "Reduced glutathione and Trolox (vitamin E) as extender supplements in cryopreservation of red deer epididymal spermatozoa", Anim Reprod Sci, 135(1-4), pp.37~46 (2012)
- Barton GM, "Toll-like receptor signaling pathways", Science, 300(5625), pp.1524~1525 (2003)
- Aktan F, "iNOS-mediated nitric oxide production and its regulation", Life Sci, 75(6), pp.639~653 (2004)
- Guzik TJ, "Nitric oxide and superoxide in inflammation and immune regulation", J Physiol Pharmacol, 54(4), pp.469~487 (2003)
- Delgado AV, "Production of tumor necrosis factor-alpha, interleukin 1-beta, interleukin 2, and interleukin 6 by rat leukocyte subpopulations after exposure to substance P", Neuropeptides, 37(6), pp.355~361 (2003)
- Yoshimura A, "Signal transduction of inflammatory cytokines and tumor development", Cancer Sci, 97(6), pp.439~447 (2006)
- Beutler B, "The biology of cachectin", TNF-α primary mediator of the host response, 1989, pp.7, 625~655
- Botting RM, "Cyclooxygenase: Past, present and future. A tribute to John R. Vane (1927-2004)", J Therm Biol, 31(1-2), pp.208~219 (2006)
- Blobaum A. L., "Structural and functional basis of cyclooxygenase inhibition", J Med Chem, 50(7), pp.1425~1441 (2007)
- Takayanagi H, "Inflammatory bone destruction and osteoimmunology", J Periodontal Res, 40(4), pp.287~293 (2005)
- Takahashi S, "Follicular dendritic cell-secreted protein is decreased in experimental periodontitis concurrently with the increase of interleukin-17 expression and the Rankl", Opg mRNA ratio, 2014, pp.49(3), 390~397
- Tyagi AM, "Estrogen deficiency induces the differentiation of IL-17 secreting Th17 cells: a new candidate in the pathogenesis of osteoporosis", PLoS ONE, 7(9), pp.e44552~ (2012)
- Boyce BF, "Functions of RANKL", RANK, Arch Biochem Biophys, pp.2008, 473(2)[DOI]
- Kearns AE, "Receptor activator of nuclear factor kappaB ligand and osteoprotegerin regulation of bone remodeling in health and disease", Endocr Rev, 29(2), pp.155~192 (2008)
- Asagiri M, "The molecular understanding of osteoclast differentiation", Bone, 40(2), pp.251~264 (2007)