파슬리 추출물의 기능성 화장품 소재로서의 생리활성
Physiological Activities of Parsley Extracts as an Ingredient of Functional Cosmetics
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초록
목적: 파슬리(Petroselinum crispum) 70% 에탄올 추출물의 활성 효능을 확인하여 기능성 화장품 소재로서의 응용 가능성을 검증하고자 하였다. 방법: 파슬리를 70% 에탄올로 추출하여, 항염증 및 항노화, 미백 효과를 확인하기 위하여 총 폴리페놀 함량, 총플라보노이드 함량, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical 소거능, nitric oxide (NO) 생성, cyclooxygenase 2 (COX2), microphthalmia-associated transcription factor (MITF), tyrosinase, phospho-extracellular signal–regulated kinase (p-ERK), phospho-c-Jun N-terminal kinase (p-JNK) 발현량 억제 효과를 측정하였다. 결과: 파슬리 추출물의 플라보노이드, 폴리페놀 함량과 DPPH radical 소거능을 확인하였으며, RAW 264.7, B16F10, human dermal fibroblast (HDF) 세포에서 파슬리 추출물에 대한 세포 독성 실험을 진행한 결과, 세포독성이 나타나지 않는 범위에서 실험을 진행하였다. NO 생성 억제 및 COX2 억제효과를 확인하였고, MITF 발현량이 억제된 반면 tyrosinase 억제 효과는 나타나지 않는 것으로 확인되었다. 또한 JNK의 인산화가 억제되는 것을 확인하였다. 결론: 파슬리 추출물은 항산화 효과가 우수하고, 항염증 효과와 미백 및 주름 개선 효과를 가지는 다양한 기능성 화장품 소재로서의 가치가 있는 것으로 사료된다.
Abstract
Purpose: This study attempted to examine the active effects of 70% ethanol extracts of parsley (Petroselinum crispum) and verify its usefulness as an ingredient of functional cosmetics. Methods: Parsley was extracted using 70% ethanol and, to verify its anti-inflammatory, anti-aging, and skin-brightening effects, its total polyphenol content, and total flavonoid content, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, as well as its ability to synthesize nitric oxide (NO), suppress cyclooxygenase 2 (COX2) and inhibit microphthalmia-associated transcription factor (MITF) and tyrosinase were assessed and phospho-extracellular signal–regulated kinase (p-ERK), phospho-c-Jun N-terminal kinase (p-JNK). Results: Total polyphenol and flavonoid contents and DPPH radical scavenging activity of parsley extracts were verified. From results of cytotoxicity test on parsley extracts in RAW 264.7, B16F10, and human dermal fibroblast cells, following experiments were conducted at concentrations which cytotoxicity was not observed. The result showed good MITF inhibition, but poor tyrosinase inhibition. In addition, NO and COX2 were inhibited and phosphorylation of JNK was suppressed. Conclusion: Parsley extracts have remarkable anti-oxidant effects and are valuable as a cosmetics ingredient with anti-inflammatory, skin-brightening, and anti-aging effects.
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