오레가노 잎 추출물에 대한 항염증 및 미백 활성
Anti-inflammatory and Whitening Activity of Origanum vulgare Leaves Extract
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
In this study, the anti-inflammatory and whitening effects of hot water and 70% ethanol extracts of Origanum vulgare leaf were examined. First, the results from measurement of the total polyphenol content confirmed that OLD was 649.16 ±0.41 mg/100 g and OLE was 646.62 ±0.85 mg/100 g. Analysis of electron donating activity and ABTS radical scavenging activity was performed for measurement of the antioxidant activity, which confirmed that both extracts exerted antioxidant activity in a concentration-dependent manner. The results of measurement of tyrosinase inhibition activity showed an inhibitory effect of 41.52% for OLE at a concentration of 1,000 μg/mL. Assessment of cytotoxicity in RAW 264.7 cells and B16-F10 cells was then performed for measurement of anti-inflammatory and whitening effects at the cellular level. RAW 264.7 cells showed cell viability of more than 95% at a concentration of 500 μg/mL, and B16-F10 cells showed cell viability of more than 95% at a concentration of 50 μg/mL. Measurement of the inhibitory activity on production of NO was performed in order to verify anti-inflammatory activity. The results confirmed that production of NO was decreased by both OLD and OLE in a concentration-dependent manner compared to the group treated with LPS alone. The inhibitory activity on melanin synthesis was determined for evaluation of the whitening effect. The results indicated that melanin synthesis was inhibited by both OLD and OLE in a concentration-dependent manner compared to the group treated with α-MSH alone. In conclusion, the results confirmed the antioxidant, anti-inflammatory, and whitening effects of OLD and OLE, indicating the potential for their use as new natural materials.
Abstract
In this study, the anti-inflammatory and whitening effects of hot water and 70% ethanol extracts of Origanum vulgare leaf were examined. First, the results from measurement of the total polyphenol content confirmed that OLD was 649.16 ±0.41 mg/100 g and OLE was 646.62 ±0.85 mg/100 g. Analysis of electron donating activity and ABTS radical scavenging activity was performed for measurement of the antioxidant activity, which confirmed that both extracts exerted antioxidant activity in a concentration-dependent manner. The results of measurement of tyrosinase inhibition activity showed an inhibitory effect of 41.52% for OLE at a concentration of 1,000 μg/mL. Assessment of cytotoxicity in RAW 264.7 cells and B16-F10 cells was then performed for measurement of anti-inflammatory and whitening effects at the cellular level. RAW 264.7 cells showed cell viability of more than 95% at a concentration of 500 μg/mL, and B16-F10 cells showed cell viability of more than 95% at a concentration of 50 μg/mL. Measurement of the inhibitory activity on production of NO was performed in order to verify anti-inflammatory activity. The results confirmed that production of NO was decreased by both OLD and OLE in a concentration-dependent manner compared to the group treated with LPS alone. The inhibitory activity on melanin synthesis was determined for evaluation of the whitening effect. The results indicated that melanin synthesis was inhibited by both OLD and OLE in a concentration-dependent manner compared to the group treated with α-MSH alone. In conclusion, the results confirmed the antioxidant, anti-inflammatory, and whitening effects of OLD and OLE, indicating the potential for their use as new natural materials.
참고문헌 (28)
- Aroca P, "Melanin biosynthesis patterns following hormonal stimulation", J Biol Chem, 268, pp.25650~25655 (1993)
- Baroni A, "Structure and function of the epidermis related to barrier properties", Clin Dermatol, 30, pp.257~262 (2012)
- Blois MS, "Antioxidant determinations by the use or a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Brenner M, "The protective role of melanin against UV damage in human skin", Photochem Photobiol, 84, pp.539~549 (2008)
- Carmichael J, "Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing", Cancer Res, 47, pp.936~ (1987)
- 최수임, "생약재 추출물의 hyaluronidase 저해 및 라디칼 소거 활성 검색", KSBB Journal, 18(4), pp.282~288 (2003), 한국생물공학회
- Chuang L, "Ethanolic extract of Origanum vulgare suppresses Propionibacterium acnes-induced inflammatory responses in human monocyte and mouse ear edema models", Molecules, 23, pp.1987~ (2018)
- nitrate in biological fluids, "Anal Biochem", 1982, 131~138
- 한창희, "오레가노 종자 에탄올 추출물의 항산화 특성 및 유지 산화안정성에 미치는 영향", Food Science and Preservation, 26(2), pp.165~173 (2019), 한국식품저장유통학회
- Hosoi J, "Regulation of melanin synthesis of B16 mouse melanoma cells by 1α, 25-dihydroxyvitamin D3 and retinoic acid", Cancer Res, 45, pp.1474~1478 (1985)
- 장영아, "블루베리, 상황, 율피, 승마 복합추출물의 화장품 소재로서의 효능", 아시안뷰티화장품학술지, 15(3), pp.281~290 (2017), 한국피부과학연구원[DOI]
- 진경숙, "산초 추출물 및 분획물의 항산화, 미백 및 항염증 활성", 한국미생물·생명공학회지, 40(4), pp.371~379 (2012), 한국미생물·생명공학회[DOI]
- 김은진, "자생식물과 생약자원 추출물의 폴리페놀, 플라보노이드 함량 및 항산화 활성 탐색", 한국식품과학회지, 44(3), pp.337~342 (2012), 한국식품과학회[DOI]
- 김향숙, "참까막살 에탄올 추출물이 B16F10 흑색종 세포에서의 멜라닌합성에 미치는 영향연구", 생명과학회지, 29(9), pp.972~976 (2019), 한국생명과학회[DOI]
- 김정은, "동백 꽃 아임계 수 추출물의 항염 및 항산화 활성", 대한화장품학회지, 48(2), pp.97~104 (2022), 사단법인 대한화장품학회
- 김민지, "무화과(Ficus carica L.) 열매 추출물의 tyrosinase 및 MITF 발현 억제를 통한 미백 활성", Journal of Applied Biological Chemistry, 66, pp.204~212 (2023), 한국응용생명화학회[DOI]
- 김태훈, "비파 씨로부터 Tyrosinase 저해 활성물질의 분리", Food Science and Preservation, 16(3), pp.435~441 (2009), 한국식품저장유통학회
- 이다솜, "딸기(Fragaria ananassa var. ‘Seolhyang’) 잎 분획별 추출물의 화장품 소재 특성", 한국식품영양과학회지, 47(3), pp.271~278 (2018), 한국식품영양과학회
- Lee HJ, "In vitro anti-inflammatory and anti-oxidative effects of Cinnamomum camphora extracts", J Ethnopharmacol, 103, pp.208~216 (2006)
- 이송주, "배나무 절지 추출물의 총 페놀함량, 전자공여능 및 Tyrosinase 활성 저해 효과", 원예과학기술지, 24(3), pp.338~341 (2006), 한국원예학회
- 이영민, "발아녹미와 노루궁뎅이 버섯 균사체를 이용한 생물전환 약용 신소재의 항산화 활성 및 NO 생성 억제 효능", 한국약용작물학회지, 25(5), pp.305~314 (2017), 한국약용작물학회[DOI]
- 박장순, "오레가노 추출물의 모발성장 촉진효과", 생약학회지, 44(3), pp.275~280 (2013), 한국생약학회
- Roberta R, "Anti-oxidant activity applying an improved ABTS radical cation decolorization assay", Free Rad Biol Med, 26, pp.1231~1237 (1999)
- 류진협, "LPS로 유도한 Raw 264.7 세포에서 A.C.C. 추출물의 항염증 효과", 한국산학기술학회논문지, 18(12), pp.503~511 (2017), 한국산학기술학회[DOI]
- 서은종, "더위지기 추출물의 항산화 및 미백 효과", 한국식품과학회지, 44(1), pp.89~93 (2012), 한국식품과학회
- Singleton VL, "Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent", Methods Enzymol, 299, pp.152~178 (1999)
- Vachtenheim J, ""transcription physiology" of pigment formation in melanocytes: Central role of MITF", Exp Dermatol, 19, pp.617~627 (2010)
- Yagi A, "Inhibition of mushroom-tyrosinase by aloe extract", Planta Med, 53, pp.515~517 (1987)