아티초크 추출물의 항산화, 항염증, 미백 효능 활성에 관한 연구
Studies of Antioxidant, Anti-inflammatory and Whitening Effects of Cynara scolymus L.
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The objective of this study was to investigate the biological activities of 70% ethanol extracts from Cynara scolymus L. (CSEE). The total polyphenol content of CSEE were found to be 158.5 mg tannic acid/100 g dry weight of CSEE. To confirm the antioxidant effect, electron donating ability and ABTS radical scavenging effect were measured. As a result, the extent of electron donating and ABTS radical scavenging effect on CSEE increased in a dose-dependent manner. The tyrosinase inhibitory effect of CSEE, which is related to skin-whitening, was observed to be 36.25% at a concentration of 1,000 µg/mL. Furthermore, MTT assay was performed to confirm the cell viability in RAW264.7 and B16F10 cell lines. In both RAW 264.7 cell and B16F10 cells, the cell viability was observed more than 95% at 100 µg/mL in all extracts. The anti-inflammatory effect of CSEE on lipopolysaccharide (LPS)-induced RAW264.7 cells was also investigated. The CSEE significantly suppressed NO production in a concentration-dependent manner. In order to measure the whitening effect on CSEE, the expressions of proteins related to whitening, such as microphthalmia-associated transcription factors, tyrosinase-related proteins-1 were observed using western blot. In the section treated with the extracts, it was confirmed that the protein expression decreased in a concentrationdependent manner. In conclusion, CSEE was verified to have antioxidant, anti-inflammatory and whitening effects, and it was confirmed that it is a potential natural cosmetic material.
Abstract
The objective of this study was to investigate the biological activities of 70% ethanol extracts from Cynara scolymus L. (CSEE). The total polyphenol content of CSEE were found to be 158.5 mg tannic acid/100 g dry weight of CSEE. To confirm the antioxidant effect, electron donating ability and ABTS radical scavenging effect were measured. As a result, the extent of electron donating and ABTS radical scavenging effect on CSEE increased in a dose-dependent manner. The tyrosinase inhibitory effect of CSEE, which is related to skin-whitening, was observed to be 36.25% at a concentration of 1,000 µg/mL. Furthermore, MTT assay was performed to confirm the cell viability in RAW264.7 and B16F10 cell lines. In both RAW 264.7 cell and B16F10 cells, the cell viability was observed more than 95% at 100 µg/mL in all extracts. The anti-inflammatory effect of CSEE on lipopolysaccharide (LPS)-induced RAW264.7 cells was also investigated. The CSEE significantly suppressed NO production in a concentration-dependent manner. In order to measure the whitening effect on CSEE, the expressions of proteins related to whitening, such as microphthalmia-associated transcription factors, tyrosinase-related proteins-1 were observed using western blot. In the section treated with the extracts, it was confirmed that the protein expression decreased in a concentrationdependent manner. In conclusion, CSEE was verified to have antioxidant, anti-inflammatory and whitening effects, and it was confirmed that it is a potential natural cosmetic material.
참고문헌 (36)
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